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Citations for New England Biolabs :
1501 - 1550 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Each PCR reaction was treated with 1 µl of Exonuclease I (M0293, NEB) at 37 °C for 15 min followed by 80 °C for 20 min to inactivate the Exonuclease I enzyme.
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bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
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bioRxiv - Genomics 2021Quote: ... 1 µL of 10 U/µL polynucleotide T4 kinase and PNK buffer (NEB) in 20 µL ...
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bioRxiv - Developmental Biology 2021Quote: Plasmid pJM1 (Pceh-23_L::mKate2::rgl-1) was generated using Gibson Assembly (NEB) of PCR products ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by the addition of 1 µl RNase H (5U final, NEB M0297L) and incubation for 20 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... in 1 x Cutsmart buffer (New England Biolabs GmbH, Frankfurt am Main, Germany) for 60 min and then the enzymes heat inactivated at 80°C for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ~1 μg of tsetse saliva was treated with PNGase F (New England Biolabs). Samples were run on a 12.5% polyacrylamide gel ...
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bioRxiv - Bioengineering 2020Quote: ... a 10x Cas12-gRNA complex was prepared using 1 μM of LbCas12a (NEB) and 0.5 μM of Cy5-labeled gRNA ...
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bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
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bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB), followed by ligation reaction at 16°C for 4.5 h and then at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
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bioRxiv - Systems Biology 2021Quote: ... 25 μL of amplicons were digested with 1 μL of DpnI (NEB R0176S). 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801) ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at 37°C in the presence of CutSmart buffer (NEB). To achieve combined DNA purification and selection of high molecular weight fragments ...
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bioRxiv - Genetics 2021Quote: ... Worms were grown on OP50-1 bacteria or dam-dcm- bacteria (NEB C2925) on standard nematode growth medium (NGM ...
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bioRxiv - Microbiology 2021Quote: ... the cDNA was treated with 1 ul of RNase H (New England Biolabs) and incubated at 37 C of 20 minutes.
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bioRxiv - Microbiology 2020Quote: ... but with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB). Barcoded amplicons were then purified with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl BSA (20 mg/ml) (New England Biolabs Inc., Ipswich, MA, USA), 10 μl Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL 400 U μl-1 T4 DNA Ligase (NEB, high concentration formula) and 664 μL H2O and incubated for 120 mins at 23°C with 300 rpm slow rotation ...
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bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
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bioRxiv - Immunology 2022Quote: ... Restriction digestion was quenched by adding 1 μl proteinase K (New England Biolabs) and incubation for 2 mins at 37°C ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA3.1.mNG2(1-10) was generated through gibson assembly (NEBuilder, New England Biolabs) of a pcDNA3.1 vector (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were stopped by the addition of 1 μL of Proteinase K (NEB) and incubated at room temperature for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB, E7600S), and an Illumina NextSeq 550 75 cycle high-output kit (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
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bioRxiv - Cell Biology 2022Quote: ... with 10 mM 10x Adenosine 5’-Triphosphate (1:10, #P0756S, New England Biolabs). The reaction was incubated in a thermocycler for 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB). Only embryos exhibiting low mosaic sfGFP expression were grown to adulthood ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... 1× Phusion HF buffer and 0.4 U Phusion high fidelity DNA polymerase (NEB). PCR was conducted in a DNA Engine Tetrad 2 thermal cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture further contained 1 mM of each ribonucleotide (rNTPs, New England Biolabs), 0.4 u/µl NxGen RNase inhibitor (Lucigen) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail) with appropriate amount of MNase (New England Biolabs) and incubated at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
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bioRxiv - Genetics 2022Quote: ... then digested with 1 μL of ApeKI (New England BioLabs, Ipswitch, MA, USA). This restriction enzyme recognizes a 5 bp degenerate sequence GCWGC ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 ul RNaseOUT and 1ul T4 RNA ligase 2 truncated K227Q (NEB M0351). Samples were then washed twice with PNK buffer ...
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bioRxiv - Biophysics 2019Quote: ... before staining in SNAP dye buffer (1 μM BG-AF647 [New England Biolabs; #S9136S] ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped by addition of 1 unit apyrase (New England Biolabs) per 100 μl reaction volume and incubation at 30°C for 10 min (sample t=0) ...
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bioRxiv - Immunology 2020Quote: ... 1 mM EDTA) and phosphorylated using T4 Polynucleotide Kinase (New England BioLabs M0201L) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids (1 ug) were linearized using BamHI restriction enzyme (NEB, Ipswich, MA, USA), column-purified using the DNA Clean & Concentrator −5 Kit (Zymo Research Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 mM SAM and 1 μl Vaccinia capping enzyme (10 units/μl, NEB). The reaction was incubated at 37 °C for 1 h and the capped RNA was purified by phenol chloroform extraction and ethanol precipitation at −20 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... 8 μL of 5 M NaCL and 1 μL of RNase A (NEB) were added to every 200 μL of eluate and to the WCE samples (diluted to 200 μL with Elution buffer) ...
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bioRxiv - Microbiology 2021Quote: ... The RT reaction was treated with 1 μl RNase H (New England Biolabs) and incubated at 37°C for 20 min to remove RNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... Quick-Load 1 kb Extend DNA ladder or Low Range PFG Marker (NEB).
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bioRxiv - Cell Biology 2019Quote: ... Chromatin was then solubilized by DNase I digestion (1 unit, New England Biolabs) in CSK buffer with Complete Protease Inhibitor for 15 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 µg of purified DNA was fragmented using dsDNA Fragmentase (New England Biolabs). Then ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2□ μl of 1× NEB Next Cell Lysis Buffer (New England Biolabs). FACS sorting was performed with a BD Influx sorter (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... 115 nL of DpnI mix (1× NEB CutSmart buffer, 0.1 U NEB DpnI) was added ...