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Citations for New England Biolabs :
1551 - 1600 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 115 nL of DpnI mix (1× NEB CutSmart buffer, 0.1 U NEB DpnI) was added ...
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bioRxiv - Genomics 2021Quote: ssDNA-specific Digestion: 20µL cfDNA was combined with 3µL Exonuclease 1 (NEB, M0293S), 3µL 10x Exo 1 Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% SDS and 1.6 U/µl of proteinase K (New England Biolabs # P8107S) to reverse the crosslinks ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by addition of 1 μL proteinase K (NEB, P8107S), followed by 15 min incubation at room temperature and addition of 20% formaldehyde) ...
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bioRxiv - Systems Biology 2021Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S), and an Illumina NextSeq 550 75 cycle high-output kit (Illumina 20024906) ...
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bioRxiv - Developmental Biology 2021Quote: ... The purified RNA was dephosphorylated with 1 µL of T4 polynucleotide kinase (NEB) in 1x T4 PNK buffer for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 87 ng template DNA and 1-units Phusion DNA Polymerase (NEB, Evry, France). The PCR product was cloned using the hot fusion method (Fu et al. ...
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bioRxiv - Biophysics 2021Quote: ... by adding 1 µl (20 units) of BamHI restriction enzyme (New England Biolabs) to 1 µg DNA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 1 μM SNAP-cell 647-SiR (New England Biolabs) for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... 20% PEG8000 and 20 U T4 RNA Ligase 1 (New England Biolabs, M0204) at 16°C for 12 hours.
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bioRxiv - Genomics 2020Quote: ... and 1 μL of Phi29 (Catalog number: M0269L Vendor: New England Biolabs Inc). The combined products are cleaned at a 0.5x bead ratio and eluted in 10 μl of nuclease-free water.
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bioRxiv - Biophysics 2020Quote: ... 1 µl of Universal nuclease (Pierce; 125U) and 10 of DNaseI (NEB; 20U) were added to the sample and incubate for 10 min at RT ...
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bioRxiv - Genomics 2020Quote: ... The reaction mix included 1 μl of 10x poly(A) polymerase buffer (NEB), 0.1 mM of ATP ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested nuclei were ligated with 1 μl of T4 DNA ligase (NEB) in 100 μl of 1 × T4 ligation buffer for 24 h at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA 5’ adaptor was ligated using T4 RNA Ligase 1 - high concentration (NEB) and 10 mM ATP ...
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bioRxiv - Immunology 2020Quote: ... 5µl Quick-Load Purple 1 kb DNA ladder (New England Biolabs, Ipswich, Massachusetts) was used as reference ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... 1.35 μL MNase (New England Biolabs, diluted 1:10 in MNase reaction buffer) was added to the resuspended chromatin and incubated at 37°C ...
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bioRxiv - Genetics 2020Quote: ... we prepared PCRs in final 25 μl volumes consisting of 1× buffer (NEB Hot Start Taq® 2X Master Mix ...
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bioRxiv - Microbiology 2022Quote: ... save for a 1 hour incubation with 0.3μL (3U) of DpnI (NEB R0176) at 37°C instead of using PspGI ...
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bioRxiv - Biochemistry 2022Quote: ... 1 uM (in ntds; ~100 pM molecules) bacteriophage lambda DNA (New England Biolabs), 25 nM fSSB (tetramer ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 mM ATP with 60U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB, Set 1 #E7335S, Set 2 #E7500S). ChIP libraries were done following NEB’s guidelines (NEB ...
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bioRxiv - Genomics 2022Quote: ... Samples were treated with 1 µl of Exonuclease I (New England Biolabs, M0293S) and subsequently pooled for SPRI bead cleanup ...
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bioRxiv - Microbiology 2022Quote: ... After the ligation of adaptors using T4 RNA ligase 1 (New England Biolabs), reverse transcription was executed ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR-1 was carried out using 2X Taq polymerase mastermix (New England BioLabs) while PCR-2 and PCR-3 were carried out using dATP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... was digested for 1 h with 50 units of DpnI (New England Biolabs) to eliminate unreplicated plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 unit of Phusion Polymerase were mixed with 1x HF Phusion buffer (NEB) and made up to 50 µl total volume with H20 ...
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bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
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bioRxiv - Systems Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB EE7600S), and an Illumina NextSeq 550 75-cycle high-output kit (Illumina #20024906) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
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bioRxiv - Synthetic Biology 2022Quote: ... into backbones previously digested with corresponding restriction enzymes (NEB, 1 h 37° C) to yield intermediate plasmids containing individual transcriptional units ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL was used in a 10 µL Kinase/Ligase/DpnI (KLD, NEB) reaction and incubated at room temperature for 10-20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μg BSA per well dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 μl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ul of 10x Template Switching Reverse Transcriptase (New England Biolabs, MA, USA) for the synthesis of the 1st strand ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were taken up in 520 µl of 1× DpnII buffer (NEB, R0543M) and incubated with 7.5 µl of 20% SDS for 1 hour shaking at 600 r.p.m ...
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bioRxiv - Genetics 2024Quote: ... for 1 hour and then treated with 1.6 units of Proteinase K (NEB) for a further hour before cleanup of DNA with AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Adaptor ligation was performed with a 1:10 dilution of adaptor (NEB, E6440S). For DNA purification ...
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bioRxiv - Neuroscience 2024Quote: ... the construct was diluted 1:5 with empty pUC19 vector (New England Biolabs) and then transfected with 7.5 µL of TransIT-293 and 2.5 µg of DNA ...
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bioRxiv - Cell Biology 2024Quote: ... and/or 1 µM SiR-647-SNAP-dye (New England Biolabs, Waltham, MA) or 100 nM DAPI (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Genomics 2023Quote: ... were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... Beads were re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S) was used for assigning indices to all samples prior to pooling ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, 200,000 units/ml) and incubated at 16°C for 14 h ...
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bioRxiv - Molecular Biology 2023Quote: ... the extracted cDNA was then added to 1 x Terminal Transferase buffer (NEB), 250 µM CoCl2 (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... Oligo 1 and oligo 2 were annealed by T4 polynucleotide kinase (NEB, #M0201S) at 37 ℃ for 30 min and 95 ℃ for 5 min ...