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Citations for New England Biolabs :
1801 - 1850 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 1 μL of 200 U M-MuLV reverse transcriptase enzyme (B0253S, New England Biolabs). Negative controls were reactions without the RT enzyme and without sample ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and treated amplicons were visualized on ~1% agarose gels with 100 bp DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Microbiology 2021Quote: ... 0.7 μL of 20 mg mL-1 bovine serum albumin (New England Biolabs, MA, USA), 0.75 μL of each primer (10 μM) ...
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bioRxiv - Immunology 2020Quote: DNA encoding SARS-CoV-1 VH regions was amplified by PCR (using Q5 polymerase NEB). J segments were modified as required to match the following protein sequence (GTLVTVSS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were undertaken in 25 ul consisting of 1 x Long amp Taq buffer (NEB), 300 μM dNTP mix ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were mixed with 1× SDS–PAGE sample loading buffer (New England Biolabs # B7703S) and incubated at 98°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: We next linearized the vector pscAAV-Hsp68-EGFP (Supplemental Figure 1) using PacI (NEB #R0547L) and AscI (NEB #R0558L) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs, USA) at 65 °C ...
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bioRxiv - Developmental Biology 2021Quote: A working lysis buffer solution was prepared by adding 1 µL of the MNase (NEB) [1:50 dilution] per 5 µL lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (NEB #E7600) (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of DNase treated RNA was reverse transcribed with Protoscript II (New England Biolabs) and a no reverse transcriptase (no RT ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μL 10× Poly(A) Polymerase Reaction Buffer and 1 μL Poly(A) Polymerase (NEB) for poly(A ...
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bioRxiv - Genomics 2021Quote: ... at specific sequence motifs (GCTCTTCN^) in the presence of 1 μl 10X buffer 3.1 (NEB) for 2 h at 50 °C and ultra-pure water to a volume of 10 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Cell Biology 2022Quote: ... and the amplicons were extracted from a 1% agarose gel (Monarch Gel Extraction Kit, NEB). Inserts were then cloned into a pCDH1 lentiviral vector ...
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bioRxiv - Biochemistry 2022Quote: ... The solution was diluted to 200 μL to yield 1X NEBuffer 1 (NEB, Ipswich, MA) at pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... Nucleotide sequence corresponding to residues 1-332 of Rv3881c was amplified using Phusion polymerase (NEB) and cloned into pET28a between NdeI and HindIII restriction sites ...
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bioRxiv - Bioengineering 2022Quote: ... 200 Ω) and cells were recovered in 1 mL SOC medium (New England Biolabs, #B9020) at 30 °C and for 90 min ...
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bioRxiv - Bioengineering 2022Quote: ... 400 U of T4 DNA ligase (1 µL of 400 U/µL) (NEB; catalog # M0202L), and 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Genomics 2022Quote: ... The genomic DNA was subsequently fragmented with 200 nl 1 U NLAIII (New England Biolabs) in 1x Cutsmart (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... The genomic DNA was subsequently fragmented with 200 nl 1 U NLAIII (New England Biolabs) in 1x Cutsmart (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 μM DNA annealed oligos and 1 unit/μL T7 RNA polymerase (New England Biolabs). Reactions were incubated at 37°C for 5 hours ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S). In order to increase input DNA above single library limits (1 μg ...
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bioRxiv - Genomics 2022Quote: The cDNA reaction products were treated with 20 units of Exonuclease 1 (New England Biolabs) and subsequently purified and concentrated using AMPure XP beads ...
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bioRxiv - Molecular Biology 2023Quote: ... chilled on ice and then combined with 30 U T4 RNA Ligase 1 (NEB M0437M), 40 U Murine RNase Inhibitor (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.125 μl of Shrimp Alkaline Phosphatase (1 U/ml; New England Biolabs, cat. no. M0371S), 0.0125 μl Exonuclease I (20 U/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Successful reactions were directly incubated at 37 °C with 1 µL of DpnI (NEB, #R0176L) for 30 min to 3 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we added 1 uL of T4 DNA ligase buffer (New England Biolabs, USA, product #B0202S). Using a thermal cycler (C1000 Touch Thermal Cycler ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to fill in the barcode oligo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step two plasmid library was linearized with SpeI-HF (NEB #R3133) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of one plasmid was digested overnight with 10 units PacI (New England Biolabs) in 20 μL at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was synthesized from 1 μg of RNA using M-MuLV Reverse Transcriptase (NEB) and random hexamer primer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% Igepal CA-630 and 5% glycerol) with 1 U/µL murine RNase inhibitor (NEB), 0.1 µg/µL T7 RNA polymerase (purified in-house ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA was treated with 10 units Exonuclease V (New England Biolabs) in a 50 μl reaction at 37°C for 30 min to remove ssDNA ...