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Citations for New England Biolabs :
1351 - 1400 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
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bioRxiv - Microbiology 2021Quote: ... T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units/μl) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs.) This target mix was then loaded on to the chip by placing 10 μL of the mix on the sticky side of the PCR film (MicroAmp ...
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bioRxiv - Cell Biology 2020Quote: ... as well as the 1 kb extended markers (New England BioLabs, #N3239) were used ...
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bioRxiv - Microbiology 2022Quote: ... and 1 U of OneTaq polymerase (New England BioLabs Inc., Ipswich, MA) in a final volume of 25 μl ...
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bioRxiv - Systems Biology 2022Quote: ... or Quick-Load® 1 kb Extend DNA Ladder (New England Biolabs) was used for the reference of DNA length ...
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bioRxiv - Biochemistry 2022Quote: ... The hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
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bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
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bioRxiv - Neuroscience 2022Quote: ... augments Cap-specific 5’ adapter ligation by T4 RNA ligase 1 (NEB)(Hetzel et al. ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL T4 RNA Ligase Buffer (New England BioLabs; Ipswitch, MA, USA), and 40 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... 10 units T4 RNA Ligase 1 (New England BioLabs; Ipswitch, MA, USA), 1 μL T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... 1×T4 DNA Ligase Buffer and 800 U T4 DNA ligase (NEB). Then heat inactivation was performed for BcgI at 65 °C for 20 min.
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 46.5 μl of 1× Quick ligation buffer (NEB, M2200). 2.5 μl of DNA Quick ligase (NEB ...
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bioRxiv - Microbiology 2021Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/μL) ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with 1% Bovine Serum Albumin (BSA, New England Biolabs, B9000S) in PBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 7 μl PEG8000 and 1 μl T4 RNA ligase 2 K227Q (NEB) at 25 °C for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.5 μl of 800 U.ml−1 proteinase K (New England Biolabs P8107S) was then added to each sample ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-Cell® 647-SiR (SiR-SNAP 1:2000) (New England Biolabs) was used to detect SNAP-tagged alleles ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U of Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and nuclease-free H2O (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U of Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and nuclease-free H2O (Ambion ...
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bioRxiv - Developmental Biology 2020Quote: ... and amplification was performed in 1 × next PCR master mix (NEB, M0541S) and 1.25 μM of custom Nextera PCR primers 1 and 2 with the following PCR conditions ...
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bioRxiv - Synthetic Biology 2020Quote: ... 20 U µL−1 of DpnI restriction enzyme (New England Biolabs, USA) were added to digest possible methylated DNA left and the mixture was incubated for an additional 15 min at 37 °C.
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bioRxiv - Microbiology 2019Quote: ... 1 µg of RNA digested with DNaseI (NEB, Frankfurt a. M., Germany) was used for reverse transcription of mRNA with the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM dNTPs and 200 U M-MuLV (New England BioLabs, USA) which were treated following manufacturer’s instructions with some modifications ...
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bioRxiv - Genetics 2020Quote: ... Only 1 U/μl I-SceI enzyme 5 X/μl buffer (NEB) were mixed when co-injecting with the HDR donor ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was loaded on four 1-ml chitin columns (NEB S6651S). Columns were washed with 10 ml HEGX ...
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bioRxiv - Physiology 2021Quote: ... sfRNA fragments were circularized using T4 RNA ligase 1 (New England Biolabs) at 4 °C overnight (Filomatori et al. ...
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bioRxiv - Microbiology 2021Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/µL) ...
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bioRxiv - Microbiology 2021Quote: ... Immediately followed by enzymatic treatment of 1 mg/mL proteinase K (NEB) at 60°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... with NEBnext Multiplex Oligos for Illumina (Primer Set 1) (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2020Quote: ... Separately seeded cells were transfected with pCMV-GLuc2 plasmids (1 μg, NEB) and GLuc activity was measured using the same kit ...
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bioRxiv - Biochemistry 2019Quote: ... were ligated to the acceptor RNA using T4 RNA ligase 1 (NEB) (Fig ...
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bioRxiv - Zoology 2019Quote: ... 1 µL Q5® High-Fidelity DNA Polymerase (New England BioLabs, USA), 2.5 µL forward primer (SSP_COX1_F) ...
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bioRxiv - Genomics 2021Quote: ... in combination with NEBNext Multiplex Oligos for Illumina Set 1 (NEB#E7335), ribosomal RNA depletion was performed using 1 μg RNA using NEBNext rRNA Depletion kit (NEB#6310) ...
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bioRxiv - Genomics 2021Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204). The 5’ adaptor contained a 6-nucleotide unique molecular identifier (UMI ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hour followed by the addition of 1µl of CIP (NEB) and incubated at 37°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... NEBNext Multiplex Oligos for Illumina Dual Index Primer Set 1 (NEB, E7600S) was used for library construction ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs)
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bioRxiv - Genetics 2021Quote: ... 1 µl restriction enzyme (BbsI_HF or BsaI_HFv2 NEB R3559L and R3733L respectively), 1 µl of SIC (pM37_T2AGAL4 or pM37_KozakGAL4 at 290 ng/ µl or 265 ng/ µl respectively) ...
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bioRxiv - Immunology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB, E7335S) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... This was then treated with 1 U Endo Hf (New England Biolabs) per 1 μg of NF155 ...
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bioRxiv - Immunology 2022Quote: ... a 1 in 200 dilution of SNAP-Oregon cell permeable ligand (NEB) was added.
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 1 mM vanadate (New England BioLabs, Ipswich, MA.).
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bioRxiv - Genomics 2022Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204). The 5’ adaptor contained a 6-nucleotide unique molecular identifier (UMI ...
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bioRxiv - Genomics 2022Quote: ... 3′ Adapter ligation was done using T4 RNA Ligase 1 (NEB, M0204L). A first binding to streptavidin beads (NEB ...
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bioRxiv - Genomics 2022Quote: ... Libraries were dual-indexed (NEBNext Dual Index Primers Set 1, NEB E7600S), and sequenced on an Illumina NextSeq 2000 instrument with P3 300 cycle reagents ...
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bioRxiv - Genomics 2022Quote: ... two adaptor ligations using T4 RNA Ligase 1 (catalog no. M0204; NEB) and reverse transcription using SuperScript III Reverse Transcriptase (catalog no ...