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Citations for New England Biolabs :
1401 - 1450 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimised to Drosophila codon suing Genewiz (USA ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Immunology 2021Quote: ... The fragments were assembled using Hi-Fi DNA Assembly Mix (NEB). For the DonorgRNA vector ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: His-MBP-Fbp17SH3 was precipitated by amylose beads (NEB; cat#E8021L). After purification ...
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
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bioRxiv - Microbiology 2020Quote: ... coli DNA Polymerase (Enzymatics) with dUTP incorporation (1x NEB buffer 2 ...
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bioRxiv - Molecular Biology 2021Quote: Experiments were performed in Escherichia coli DH5-α (NEB), grown in LB-Lennox (Oxoid ...
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bioRxiv - Genomics 2020Quote: ... coli DNAs were incubated in 1x CutSmart buffer (NEB) with 1 μl (20 U/μl ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21 DE3 cells (New England Biolabs, Ipswich, MA). Cells were grown in LB media (VWR ...
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bioRxiv - Genomics 2020Quote: ... coli poly(A) polymerase (New England Biolabs, Cat. # M0276S). The mixture was incubated at 37°C for 10 min ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 [DE3] or NEB Express (New England Biolabs) and lysed in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3) cells (New England Biolabs, Ipswich, MA) and expressed and purified as previously described for SP (REF) ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-Beta electrocompetent (New England Biolabs, MA, USA) or in-lab made equivalent for increased transformation efficiency ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3) chemically competent cells (NEB product # C2530H). The following day ...
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bioRxiv - Biophysics 2019Quote: ... the Escherichia coli BL21 (DE3) strain (NewEngland Biolabs, USA) was transformed with this vector ...
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bioRxiv - Biochemistry 2020Quote: ... coli NiCo21 (DE3) cells (New England Biolabs, Ipswich, MA) grown in TBmod plus MOPS medium supplemented with 25μg/ml kanamycin and no biotin were used ...
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bioRxiv - Bioengineering 2020Quote: ... coli (High Efficiency) (New England Biolabs, Inc., Ipswich, MA) and extracted using the EndoFree Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) using Q5 polymerase (New England Biolabs) and the following primers ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21 (DE3) (New England Biolabs, Ipswich,MA,USA) and purified using HisPur™ Ni-NTA Resin (Thermo-Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... coli using the NEB® PCR Cloning Kit (NEB).
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bioRxiv - Immunology 2021Quote: ... coli according to the manufacturer’s protocol (New England Biolabs). Vectors containing CD4 and heavy chain genes were then sequenced and compared with the original heavy chain sequences ...
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bioRxiv - Synthetic Biology 2021Quote: Escherichia coli DH10B (New England BioLabs, Ipswich, MA, USA) was used for all routine cloning and directed evolution ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21 DE3 (New England BioLabs, Ipswich, MA, USA) was used for protein expression ...
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bioRxiv - Microbiology 2020Quote: ... coli were digested with XhoI (New England Biolabs, US) and EcoRI for 1 hour at 37°C ...
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bioRxiv - Genomics 2022Quote: ... coli topoI were made with a commercial topoI (NEB).
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bioRxiv - Microbiology 2022Quote: ... coli strain DH5α (New England Biolabs, Ipswich, MA, USA) was used for sub-cloning procedures and plasmid purification ...
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bioRxiv - Microbiology 2022Quote: ... coli strain DH5α was obtained from NEB (cat. C2987I). E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli strains: NEB 5-alpha (NEB C2987, not authenticated), BL21-AI (ThermoFisher C607003 ...
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bioRxiv - Microbiology 2021Quote: ... coli C2987 chemically competent cells (New England Biolabs, Inc). pUC19::PBP7 was used as a template for Pfu-mediated deletion mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) cells (New England Biolabs, cat#C2527I) following manufacturer recommendations and selected on LB+CA100 before maintenance as a 15% glycerol stock at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... coli DH10B (New England Biolabs 10-beta, cat#C3020K), selected on LB supplemented with 50 μg/ml kanamycin (LB+KA50) ...
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bioRxiv - Immunology 2020Quote: ... coli for plasmid propagation were purchased from NEB (#C2987H).
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) at 37 °C in 5 mL LB medium containing 25 μg mL−1 kanamycin by induction with 1 mM IPTG for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) as described in section “Proteolytic inhibition assays” ...
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bioRxiv - Microbiology 2022Quote: ... coli holoenzyme RNA polymerase (New England Biolabs, Ipswich, MA). Where indicated ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB-5α (New England Biolabs, Beverley, MA) was grown in Luria broth (LB ...
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bioRxiv - Biochemistry 2019Quote: ... coli NEB5α and BL21 (DE3) were purchased from NEB. Antibiotics were used at the following final concentrations ...
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bioRxiv - Cancer Biology 2019Quote: ... coli XL1-blue supplemented with helper phage M13K07 (NEB) and incubated over night at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... coli RNA polymerase Core enzyme (2.5 µg) (BioLabs #M0550S) at 37 ºC for 2 h ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3) chemically competent cells (NEB product # C2530H) were transformed with 100 ng of pET24a His8–MBP plasmid (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... coli strain NEB5α (New England Biolabs, Ipswich, MA, USA) was grown in Luria−Bertani (LB ...
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bioRxiv - Plant Biology 2021Quote: ... coli cells (DH5) carrying pMAL-c5x (New England Biolabs) or pMAL-NbERF-IX-33a were cultured overnight at 37°C in rich medium (1% tryptone ...
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bioRxiv - Synthetic Biology 2020Quote: ... Escherichia coli BL21 (DE3) cells (New England Biolabs, USA) were used for recombinant protein expression ...