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Citations for New England Biolabs :
1301 - 1350 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Biochemistry 2021Quote: ChIP-seq library construction was performed using NEBNext ChIP-Seq Library Prep Master Mix Set (E6240, NEB, Ipswich, MA) following the manufacturer’s instruction with modifications ...
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bioRxiv - Plant Biology 2020Quote: Flax small RNA libraries were constructed using the NEBNext Small RNA Library Preparation set for Illumina (NEB, cat # E7300); for columbine and rose ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were constructed using the NEBNext mRNA Library PrepReagent Set for Illumina (New England Biolabs, Ipswich, MA USA) following the manufacturer’s protocol and were subjected to Illumina sequencing ...
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bioRxiv - Neuroscience 2021Quote: Twelve small RNA libraries representing 2 biological and 3 technical replicates from control- or BDNF-treated cortical neurons and corresponding EVs were prepared using NEBNext_Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) as per manufacturer’s instructions and sequenced in Illumina HiSeq2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs) and sequenced on an Illumina Hiseq4000 ...
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bioRxiv - Genomics 2022Quote: ... ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs). Rabbit polyclonal antibody for Rad21 has been described in39 ...
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bioRxiv - Cancer Biology 2022Quote: The same sets of RNA samples were reverse transcribed using Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). cDNA was measured using a QuantStudio 7 Flex Real-time PCR machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The sequencing library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA), with added index codes to attribute sequences of each sample as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Illumina libraries were then prepared using the NEBNext DNA Sample Prep Master Mix Set (New England Biolabs, MA, USA) and DNA fragments were PCR-amplified using Platinum Pfx DNA polymerase (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... PCR analysis was carried out with different primer sets (Supplementary Table 1, Fig. 2B) using Phusion High-Fidelity DNA Polymerase following the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Genomics 2021Quote: ... DNA libraries were constructed using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S). To elute DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). The required number of PCR cycles to amplify library is determined by running PCR for 10-15 cycles and analyzing PCR products on a 6% PAGE gel ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent™ (New England BioLabs), diluted to the same concentration and pooled ...
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bioRxiv - Neuroscience 2022Quote: ... and libraries were prepared using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England BioLabs, UK) and the Ion Xpress Barcode Adapters 1-96 Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... We pooled the second set of eBlocks at equal molar ratio and used Esp3I Type IIs restriction enzyme (NEB), NEBridge Ligase Master Mix ...
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bioRxiv - Genetics 2023Quote: ... Small RNA libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: Libraries were prepared according to the manufacturer’s protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reaction was set up with 1 µL of the supernatant in a 12.5 µL reaction with Taq polymerase (NEB), according to manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... sequencing libraries were generated using a NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB) and 5% DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA libraries were then prepared using a range of mitochondrial RNA inputs (final input range 1.8-120 ng) using the NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs). To investigate the efficiency of small RNA library preparation across a range of mitochondrial RNA inputs ...
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bioRxiv - Cancer Biology 2024Quote: ... and sequencing libraries were generated using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB E7330). Libraries were pooled and sequenced using NextSeq 500 High Output ...
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). A double digest using PacI and AscI was done on the PCR products and the pcs2+MCS-P2A-sfGFP plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was PCR amplified with Phusion High-fidelity PCR mastermix (NEB M0530) and Truseq PCR primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Q5 PCR Mix (NEB), 5 ng template and appropriate primers in 20 μl reactions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB) and sequenced using the Sanger method with M13F or M13R primers.
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bioRxiv - Microbiology 2020Quote: PCR amplification was performed using high-fidelity Q5 Polymerase PCR (New England Biolabs) with the following primers sets ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR product was purified using Monarch® PCR & DNA Cleanup kit (NEB) and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR amplifications were column-purified using the Monarch PCR & DNA Cleanup Kit (NEB), and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB) and sent for Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned using the Monarch PCR & DNA cleanup kit (NEB, T1030L). VSG sequences were determined by amplicon nanopore sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation or Sanger sequencing with the sequencing primer (5’-AGAGAATACTAAGCTAGTTGGC-3’ ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030). Plasmids were transformed into electrocompetent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR-amplified or digested products were purified (Monarch PCR & DNA Cleanup Kit, NEB). Then ...
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Genomics 2024Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (NEB, T1030S) following the Manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB) and checked on 1% agarose gel and approximately 600 ng of each PCR product was used as template for in vitro dsRNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL RT Adapter (RTA)(SQK-RNA002) and 2 μL T4 DNA Ligase (NEB) were mixed together and incubated under 25 °C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... the RT reaction mixture was diluted 40x into a HotStart Taq (New England Biolabs) PCR reaction (1 mM MgCl2 ...