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Citations for New England Biolabs :
1301 - 1350 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and 200 µg/ml RNAse A (New England Biolabs #T3018-1)) for four hours on ice ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...
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bioRxiv - Genomics 2023Quote: ... 0.64 μL 10 U μL-1 T5 exonuclease (New England Biolabs), 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 1 μL rCutSmart™ Buffer (New England BioLabs, MA, USA) and incubated for 2-4 hours at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... 16ul nuclease-free water and 1 μl T4 DNA ligase (NEB). The entire volume of the reaction was pipetted on a parafilm sheet placed on a glass plate and the coverslips were inverted over it ...
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bioRxiv - Genomics 2024Quote: ... Unmethylated λ-bacteriophage DNA (1:200 mass ratio; New England Biolabs) was included in all samples to calculate unmethylated cytosine conversion efficiency ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
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bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
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bioRxiv - Cancer Biology 2024Quote: ... made up of 1 uL DNA Ladder (New England Biolabs, N3231L), 1 µL Purple Dye (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog #M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Molecular Biology 2024Quote: ... steps 1–3: GELase was replaced by β-agarase I (NEB). The reaction (1 U per plug ...
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bioRxiv - Bioengineering 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, 7335S) were used for the final PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... we supplemented the reaction with 1 U micrococcal nuclease (New England Biolabs) and allowed it to incubate for 10 min ...
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bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
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bioRxiv - Genetics 2021Quote: ... and the sample was digested with DNase I (NEB [1:20 dilution]) for 30 seconds at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... and 30 μg was diluted 1:3 in gel loading buffer (NEB), sonicated ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
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bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl ProtoScript II RT (200 U/μl, New England Biolabs) to a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U Q5 hot start high fidelity DNA polymerase (New England Biolabs), 500 nM each primer (Table 2 primers #1-4 for 1116NS19.9 or primers #5-8 for 5b1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was digested in situ using restriction enzyme CviKI-1 (NEB, R0710L) and barcoded using RELACS custom barcodes (4bp UMI + 8bp RELACS barcode ...
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bioRxiv - Pathology 2019Quote: ... 1 µl of either SP6 or T7 polymerase (New England Biolabs, USA). SP6 reactions were incubated at 40°C for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 μL of Index (X) Primer (25 mmol) (NEB, Ipswich, USA). The reaction conditions were as follows ...
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bioRxiv - Pathology 2019Quote: ... 1 μL (2 units) DNAse I (M0303S, New England BioLabs, Ipswich, MA), and 89 μL nuclease-free H2O ...
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bioRxiv - Cancer Biology 2019Quote: ... and the cell pellets were resuspended in 345μl of 1× NEBuffer2 (NEB) per 5 million cell aliquot ...
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bioRxiv - Microbiology 2020Quote: ... 0.8 mg ml-1 bovine serum albumin (BSA, New England Biolabs GmbH), 0.2 mM of each dNTP (Bioline ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... digested vectors by a 1:2 mixture of T4 ligase (NEB, M0202L) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Microbiology 2019Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/µL) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μL DNA Pol I and 20 U DNase I (NEB, U.K.), 1 mM each of dATP ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides (1) and (4) were phosphorylated with T4 PNK (New England Biolabs) to allow ligation to the backbone fragment ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-Phospho-histone H3 1:100 (New England Biolabs, Ipswich, USA) and secondary antibodies AlexaFluorTM 488 goat anti-chicken and AlexaFluorTM 594 goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... augments Cap-specific 5’ adapter ligation by T4 RNA ligase 1(NEB). The 3’ adapter was ligated using truncated T4 RNA ligase 2 (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... The membrane was incubated with HRP-conjugated secondary antibodies (1:5000, NEB) for 45 mins at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of T4 RNA ligase I (10U final, NEB M0437M) were combined in 20 μl total volume and incubated for 16 h at 16 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µL of 10mM dNTPmix and 2 µL of Phusion polymerase (NEB). PCR products were purified using the QIAquick PCR purification column and eluted with 30 µL Qiagen Elution Buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of 10-mM deoxynucleoside triphosphate mix (NEB, Ipswich, Massachusetts, USA), 1 μl of each 25-μM primer ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SNAP (1:1000, polyclonal, New England Biolabs, Ipswich, MA, P9310S), or mouse anti-tubulin (1:40,000 ...
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bioRxiv - Biochemistry 2021Quote: MtDNA was treated with 1 μl uracil-DNA glycosylase (UDG) (NEB, M0280), 1.5 μl Endonuclease IV (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... overnight using 40 units of T4 RNA ligase 1 (New England Biolabs) in PNK buffer with RNase inhibitors at 16°C ...
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bioRxiv - Systems Biology 2021Quote: ... Beads were resuspended at 5 µg µl-1 followed by MseI (NEB) digestion according to the manufacturer’s instructions ...