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Citations for New England Biolabs :
1051 - 1100 of 6948 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: RT-LAMP reactions were carried out with the WarmStart LAMP Kit (NEB) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
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bioRxiv - Microbiology 2021Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with an in-house developed protocol ...
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bioRxiv - Microbiology 2020Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using LunaScript® RT SuperMix Kit (New England Biolabs). Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... and reverse transcription performed using LunaScript RT SuperMix (E3010, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Luna Universal One-Step RT qPCR Master Mix kit (NEB E3005L) and Tween-20 (Sigma-Aldrich P1379) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg RNA was converted into cDNA using ProtoScript II RT (NEB), oligo(dT)s and Murine RNAse inhibitor (NEB ...
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bioRxiv - Microbiology 2022Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesised using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR (Luna® Universal qPCR Master Mix; New England BioLabs; M3003L) was performed in 384-well plates (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 µL of Template Switching RT Enzyme Mix (NEB Catalog# M0466L). After mixing ...
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bioRxiv - Synthetic Biology 2023Quote: ... which was carried out using the LunaScript RT SuperMix Kit (NEB, USA). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCRs were performed using Luna® Universal qPCR Master Mix (NEB) and acquired on a QuantStudio 3 system (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019) or the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006 ...
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bioRxiv - Biochemistry 2023Quote: ... and the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006) were used to detect the N1 (FAM ...
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bioRxiv - Biochemistry 2023Quote: ... or the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006) following the standard NEB protocol or SalivaDirect RT-qPCR amplification protocol as indicated ...
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bioRxiv - Biochemistry 2023Quote: ... RT-qPCR was performed using the Luna Universal qPCR Master Mix (NEB) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: cDNA conversion was performed using ProtoScript II RT standard protocol (NEB, #M0368) and Random Primer Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using Luna Universal qPCR Master mix (NEB #M3003) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Reverse transcription was done using DNase-treated RNA using MMLV RT (NEB) according to the manufacture protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was carried out using LunaScript RT (New England Biolabs E3010) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Molecular Biology 2019Quote: PCR products were amplified by PCR using the Q5 (NEB®) high-fidelity polymerase following standard conditions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Molecular Biology 2023Quote: All restriction enzymes were purchased from NEB and PCR amplification was performed with Q5 High-Fidelity PCR (NEB) following the manufacturer’s recommendations ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... were PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB) and cloned into pCFD4 (Addgene 49411 ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared by ligation of NEBNext adapters and indexed i7 primers (NEB). Libraries were amplified for 12-14 cycles and cleaned with AMPureXP beads prior to sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Unused primers were removed by the addition of exonuclease I (New England Biolabs), following which beads were washed ...
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bioRxiv - Molecular Biology 2021Quote: ... with a 1:1 combination of oligodT 18 primers and random hexamers (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Unused primers were removed by the addition of exonuclease I (New England Biolabs), following which ...
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bioRxiv - Immunology 2019Quote: ... Unused primers were removed by the addition of exonuclease I (New England Biolabs), following which beads were washed ...
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bioRxiv - Biophysics 2021Quote: ... Cloning and mutagenesis enzymes and primers were purchased from New England BioLabs (NEB) and Integrated DNA Technologies (IDT) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was quantified by qPCR using NEBNext universal and index primers (NEB E7335S). Libraries were indexed using NEBNext High-Fidelity PCR Master Mix (NEB M0541S ...
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bioRxiv - Molecular Biology 2020Quote: ... Primers used to generate mutations were designed using the NEBaseChanger tool (NEB, USA), listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... for 16 cycles using Universal primer and unique indices (NEB Catalog# E7335L, E7500L) in a total of in a 50 μl reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... with primers detailed in Table S1 followed by DpnI (New England Biolabs, R0176) digestion before bacterial transformation and colony selection.
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bioRxiv - Developmental Biology 2022Quote: ... and then phosphorylated with PNK (New England BioLabs; see Table S5 for primers). The phosphorylated fragments were then cloned into the StuI site of a pCS2+ vector treated with Antarctic Phosphatase (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... RNA primer end-labeling was accomplished using T4 polynucleotide kinase (T4 PNK, NEB) and 25 μCi of ATP ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... Primers were annealed and ligated with the digested promoter with T4 ligase (Biolabs). Cells were seeded on CELLSTAR® 96-well white plate (Greiner Bio-one ...
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bioRxiv - Genetics 2020Quote: ... and a degenerate 20-mer (primer jklab0172) was inserted by HiFi Assembly (NEB). The resulting barcoded clone pools were transformed ...
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bioRxiv - Physiology 2020Quote: ... Excess bead primers were removed by the treatment of Exonuclease I (NEBM0293S, NEB), cDNA synthesis was performed using Template Switch Oligo (TSO) ...
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bioRxiv - Biochemistry 2021Quote: ... Both forward and reverse mutagenic primers were mixed with 10x PfuUltra buffer (NEB), DNA template (40 ng) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µl Reverse External Primer (10 µM) and Nuclease-free water (NEB,USA) up to 100 µl were mixed on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The overlapping oligonucleotide primers were designed by NEBuilder Assembly Tool (New England Biolabs) and were synthesized commercially and purified on a reversed-phase column by FASMAC (Japan) ...
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bioRxiv - Bioengineering 2022Quote: ... the reverse primer adapter sequence was removed using EcoRI (New England Biolabs, #R0101L) and strand complementary to EcoRI and part of the adapter sequence (Supplementary Table 1) ...
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bioRxiv - Plant Biology 2022Quote: ... along with oligo-dT 18 mer primer (New England Biolabs, Ipswich, MA; www.neb.com) was used to synthesize cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...