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Citations for New England Biolabs :
1001 - 1050 of 6948 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... 72°C for 30s) using Illumina indexing primers (NEB Catalog No. 7500L). DNA was purified using Ampure XP beads and sequenced on Illumina HiSeq 2500 to generate 75 bp paired-end reads ...
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bioRxiv - Biophysics 2021Quote: ... Primers were designed using the NEBuilder tool (New England Biolabs, Ipswitch, MA), inserts were synthesized as gBlocks and purchased from Integrated DNA Technologies (Integrated DNA Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... with either single index or dual index primers also obtained from NEB. We followed the manufacturer’s instructions except for the following variations:
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bioRxiv - Microbiology 2021Quote: ... The primers were obtained using the NEBuilder Assembly Tool (New England Biolabs). After digestion by DpnI (New England Biolabs ...
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bioRxiv - Synthetic Biology 2021Quote: ... the forward primers were phosphorylated using T4 polynucleotide kinase (New England Biolabs) to facilitate T4 ligation later ...
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bioRxiv - Cell Biology 2021Quote: ... from pSJ1256 using primers containing the PacI and AscI (New England BioLabs) restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... excessive primers were removed with an enzymatic protocol using exonuclease I (NEB) and quick CIP (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... we reverse-transcribed cDNA with a random primer mix (NEB, MA, USA) and GoScript™ Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... Ligated fragments were amplified using dual-indexed primers for Illumina (NEB #E7600S) for 8-10 PCR cycles ...
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bioRxiv - Genetics 2020Quote: ... used dual index primers (from NEBNext Multiplex Oligos for Illumina, NEB E7600S) for multiplexing ...
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bioRxiv - Pathology 2021Quote: ... using custom synthesized complementary primers (IDT) and the Gibson assembly kit (NEB). After verifying the DNA sequence of the pcDNA3-4 fused with the cDNA for ace2 1-618-ddc-abd ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was generated using oligo (dT) 18 primer (New England Biolabs, 513165) and SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: The NNS primers were phosphorylated with T4 polynucleotide kinase (NEB, cat#M0201S). 20 μL phosphorylations was prepared according to the following recipe ...
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bioRxiv - Genomics 2021Quote: ... excess primers were removed using an Exonuclease I digestion (New England Biolabs), second strand synthesis was performed ...
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bioRxiv - Genomics 2021Quote: Purified RNA (5 ug) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described Smola et al. ...
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bioRxiv - Genomics 2021Quote: Purified RNA (9 μg) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described in Smola et al. ...
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bioRxiv - Immunology 2020Quote: ... RNA and primer dimers were digested using RNAseA and Lambda Exonuclease (NEB) after which cDNA was amplified using the Kapa Biosystems HiFi HotStart ReadyMix (2X ...
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bioRxiv - Immunology 2020Quote: ... Beads were then treated with ExoI to digest unoccupied primer sites (NEB).
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bioRxiv - Genomics 2022Quote: ... specific primers (Supplementary Table S1) were radiolabelled with T4 Polynucleotide kinase (NEB) and [?-32P]ATP (6,000 Ci/mmol) ...
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bioRxiv - Plant Biology 2022Quote: ... and ZmCYP71Z16 using gene-specific primers and Phusion polymerase (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... 20μM random primers (Thermo, SO181) and 1mM dNTP (NEB, N0446S and N0459S), then they were incubated at 98°C for 3min and immediately cooled down at 4°C for 20min ...
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bioRxiv - Molecular Biology 2023Quote: ... dinucleotide primers were 5’-labeled using T4 polynucleotide kinase (New England Biolabs) and added at 20 µM without pre-annealing to the template ...
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bioRxiv - Biochemistry 2024Quote: ... followed by cDNA synthesis with oligoT primers (ProtoScript II, New England Biolabs). The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Developmental Biology 2024Quote: ... A primer extension reaction was performed using Phusion DNA polymerase (M0530L, NEB) followed by QIAquick purification (28106 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using a random primer mix (New England Biolabs, S1330S) and ProtoScript® II reverse transcriptase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Molecular Biology 2021Quote: ... followed by a library preparation with the NEBNext Fast DNA Library Prep Set for Ion Torrent (NEB). cDNA libraries were sequenced on an Ion Torrent Proton.
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bioRxiv - Developmental Biology 2021Quote: ... Library preparation was based on the NEBNext Multiplex sRNA Library Prep Set for Illumina (New England BioLabs) with slight modifications ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs) with IonXpress Barcode Adapters (Ion Torrent ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracted algal gDNA was fragmented using three different sets of restriction enzymes (SRE) all obtained from NEB: SREI (HaeIII ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs, E7300S) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... ‘RPFs RNA’ libraries were prepared using a NEBNEXT Small RNA Library Prep Set for Illumina (E7330, NEB), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Library was prepared using NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S) according to manufacture instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP-seq library construction was performed using the NEBNext ChIP-Seq Library Prep Master Mix Set (NEB). Libraries were sequenced on the Illumina HiSeq 2000/2500 platform ...
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bioRxiv - Molecular Biology 2021Quote: ... miRNA libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragments were end-modified with adaptors using NEBNext Multiplex Oligos for Illumina Set 2 (New England BioLabs), following library amplification (PCR ...
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bioRxiv - Neuroscience 2023Quote: ... sRNA libraries were generated using the NEBNext Small RNA Library Preparation Set for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB #E7330S) following the manufacturer’s protocols as described previously36 ...
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bioRxiv - Developmental Biology 2024Quote: ... The ribosome profiling library was prepared using NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation utilized the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB Cat# E7300/7580) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England BioLabs) and primer sets validated in our lab (Supplemental Table 5) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl ProtoScript II RT (200 U/μl, New England Biolabs) to a total volume of 20 μl ...