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Citations for New England Biolabs :
951 - 1000 of 6948 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq samples were prepared with single index primers (NEB ME6609S). RNA integrity and and concentration of amplified libraries was assessed with the Bioanalyzer and Qubit ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Genomics 2021Quote: ... Post-PCR each PCR aliquot was exonuclease I (NEB) treated ...
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bioRxiv - Biochemistry 2023Quote: ... and amplified by PCR using Pfusion PCR polymerase (NEB) with specific primers or random hexamer primers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... All qPCR reactions were set up with the Luna Universal qPCR master mix (New England BioLabs) and 150 nM of each primer in a total volume of 20 μl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we generated two sets of Bionano genome maps with the enzymes Nt.BspQI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we generated two sets of Bionano genome maps with the enzymes Nt.BspQI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Using the NEBNext DNA Library Prep Master Mix Set for 454 (New England Biolabs ref: E6070) 21.25μl of DNA extract was subjected to three consecutive steps ...
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bioRxiv - Neuroscience 2021Quote: ... NEBNext Small RNA Library Prep Set (E7330) for Illumina was used per the manufacturer’s instructions (NEB). Libraries were multiplexed and sequenced on HiSeq2500 ...
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bioRxiv - Bioengineering 2021Quote: 25 µl reactions were set up following the PURExpress protocol (In Vitro Protein Synthesis Kit, NEB) in black low-volume 384-well polystyrene plates (Corning) ...
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bioRxiv - Genetics 2019Quote: ... Libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s protocol ...
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Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: ... Small RNA libraries were constructed using the NEBNext Small RNA Library Prep Set for Illumina (NEB). The sncRNA libraries were sequenced on an Illumina HiSeq4000 sequencer (SE50).
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were prepared with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB). Libraries were size selected on 10% polyacrylamide gels and sequenced on an Illumina NextSeq 500 (High Output Kit ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Linker-ligated RPFs were reverse transcribed with ProtoScript II RT (NEB) and gel-purified from 15% TBE-Urea gels ...
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bioRxiv - Cell Biology 2020Quote: ... After ligation at RT for 4 h with T4 ligase (NEB), the nuclei were pelleted ...
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bioRxiv - Immunology 2022Quote: ... 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Molecular Biology 2019Quote: ... with Luna Universal One-Step RT-qPCR reagents (New England Biolabs). 50 ng total RNA and 250 nM primers were added in each reaction ...
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bioRxiv - Synthetic Biology 2021Quote: ... RT-qPCR (Luna Universal qPCR Master Mix; New England BioLabs; M3003L) was performed in 384 well plates (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used ...
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bioRxiv - Immunology 2022Quote: ... 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Genetics 2022Quote: ... we used the Luna One Step RT-qPCR kit from NEB according to the manufacturer’s instructions at 10μl total volume in 384 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl ProtoScript II RT (200 U/μl, New England Biolabs), and 2 μl 100μM template-switching oligonucleotide TSO (Supplementary Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... or the LunaScript RT Super Mix Kit (NEB # E3010(S/L), Ipswich ...
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bioRxiv - Systems Biology 2019Quote: ... and the Luna Universal One-Step RT-qPCR Kit (NEB, E3005E) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Primer sequences used are described in Table S2 ...
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bioRxiv - Pathology 2021Quote: ... RT-LAMP reactions were performed using WarmStart LAMP Kit (NEB, USA) at a final volume of 10 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription (RT) was performed with M-MuLV reverse transcriptase (NEB) according to manufacturer instructions with a short oligonucleotide to prevent annealing of the oligonucleotides on RNAs that lost a few nucleotides at the 3’ end during the migration (and would thus run faster in the reverse run ...
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bioRxiv - Biochemistry 2022Quote: ... by Luna Universal One-Step RT-qPCR Kit (New England BioLabs) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... cDNA synthesis was performed using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using the LunaScript RT SuperMix (NEB E3010) and 1μg DNase-treated total RNA with incubation at 25ºC for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse transcribed using LunaScript® RT SuperMix Kit (NEB), and cDNA was used for PCR with primers nCoV-96_L/nCoV-96_R (Table S4 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse-transcribed using LunaScript® RT SuperMix Kit (NEB). Diluted cDNA (100-fold ...
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bioRxiv - Genomics 2023Quote: ... Viral RNA was reverse transcribed using LunaScript RT SuperMix (NEB #E3010) to generate cDNA as described (Freed et al ...
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bioRxiv - Systems Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB, #E3005). No template and genomic DNA controls were included in all experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Luna® Universal One-Step RT-qPCR Kit (NEB, Ipswich, Massachusetts) was used to quantify the RNA samples per the manufacturer’s guidelines ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... cDNA was generated using the Induro RT reverse transcriptase system (NEB). PCRs were conducted using Q5 DNA polymerase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of viral genomes in the heat-inactivated samples performed through reverse transcription quantitative polymerase chain reaction (RT-qPCR) ...
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bioRxiv - Genomics 2023Quote: Each library was converted to cDNA with Induro RT (NEB M0681S) using a 57-mer ssDNA as a primer ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA synthesis was performed using LunaScript® RT SuperMix Kit (NEB) following provider recommendation ...
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bioRxiv - Microbiology 2024Quote: ... and reverse transcription was performed with ProtoScript II RT enzyme (NEB) using barcode primers (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA was then reverse-transcribed using LunaScript RT-supermix (NEB #E3010). Genotyping PCR was then conducted using Q5 polymerase (NEB ...
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bioRxiv - Genetics 2021Quote: ... The forward primer was phosphorylated with T4 polynucleotide kinase (New England Biolabs) and used in a high fidelity PCR reaction (see below ...
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bioRxiv - Developmental Biology 2020Quote: ... by using primers with BamHI-HF (New England Biolabs, 20U/ml, #R3136S) and XbaI (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... single primer site-directed mutagenesis was performed with Q5 DNA polymerase (NEB) and primer #8964 to change the Cys21 codon (TGT ...
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bioRxiv - Genomics 2019Quote: ... Universal and index primers from NEBNext Multiplex Oligos for Illumina (NEB #E7335S) were incorporated by a 12 cycle PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 μL of Index (X) Primer (25 mmol) (NEB, Ipswich, USA). The reaction conditions were as follows ...
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bioRxiv - Systems Biology 2019Quote: ... DNA samples were amplified using Illumina indexing primers (NEB Catalog no. 7600L). PCR reactions were performed in 16 separate 20 μl aliquots on beads involving 8 cycles of amplification (98°C for 90 seconds followed by 98°C for 15s ...