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Citations for New England Biolabs :
1151 - 1200 of 6948 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared using LunaScript™ RT SuperMix Kit (New England Biolabs, E3010S). Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was obtained using LunaScript™ RT SuperMix Kit (New England Biolabs, USA) and qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was synthesized immediately afterwards (Lunascript RT Super Mix; New England BioLabs; E3010L) with 800-1000ng total RNA as input (same amount for each condition within experimental repetitions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesised using the LunaScript RT SuperMix Kit (New England Biolabs E3010) and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... each RT reaction was treated with 5000units of RNAse H (New England Biolabs) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed using Luna Universal qPCR master mix (New England Biolabs) with primers specific to 18s rRNA (forward primer 5’-GGACCAGAGCGAAAGCATTTGCC-3’ and reverse primer 5’-
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bioRxiv - Microbiology 2021Quote: ... The RT reaction was treated with 1 μl RNase H (New England Biolabs) and incubated at 37°C for 20 min to remove RNA ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed using LunaScript™ RT SuperMix Kit (New England Biolabs) and in a standard thermocycler according to manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: ... Synthesis of cDNA was performed with the LunaScript RT SuperMix Kit (NEB, #E3010L). For quantitative real-time PCR (qPCR ...
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bioRxiv - Immunology 2020Quote: ... we used the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) with 1 ug of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The Luna Universal Probe One-Step RT-qPCR Kit (Cat. No. E3006S, NEB) was used as the relevant master mix ...
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bioRxiv - Microbiology 2021Quote: ... 5’UTR was obtained using the Template Switching RT Enzyme Mix (NEB #M0466) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... and reverse transcription was performed using LunaScript RT Supermix Kit (New England Biolabs). Oligonucleotides used for qRT-PCR are listed in Supplementary Information Table S3 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs). mcr-1 and trfA (reference ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs). Amplification was performed using StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: cDNA was prepared using LunaScript® RT SuperMix Kit (New England Biolabs, #E3010L), together with no-RT control reaction and no template controls ...
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bioRxiv - Microbiology 2022Quote: ... the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) was used and the qPCR was conducted on the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)44 ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) was used ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthsised using using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... This mix includes 2.5 µL of Template Switching RT Buffer (NEB Catalog# B0466S), 0.5 µL of 25 µM Template Switching Oligo (5 µM final concentration in RT mix ...
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bioRxiv - Molecular Biology 2023Quote: ... For these analyses Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX opus real-time 384 system (BioRad ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Genomics 2023Quote: ... RNA was converted to cDNA using LunaScript RT SuperMix (New England Biolabs, 3010) in a final 10 µL reaction ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was first synthesised using the Lunascript RT Supermix (New England Biolabs, USA). qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis performed using a LunaScript RT SuperMix kit (New England Biolabs). RT-qPCR was performed on the resulting cDNA using a LightCycler 96 Real-Time PCR system (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... and PCR (NEB #M0491S) at the region of indel (forward primer ...
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bioRxiv - Immunology 2019Quote: ... PCR nucleotides (NEB, MA) and Gag-specific reverse primer (Table S3) ...
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bioRxiv - Microbiology 2021Quote: ... using Phusion PCR (NEB) and primers BAC16_downstream_of_GFP_STOP_overhang_plus_Zeo_3ʹ and BAC16_upstream_of_GFP_ATG_antisense_strand_overhang_plus_EM7_P_start and inserting it into BAC16 via recombination ...
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bioRxiv - Cell Biology 2022Quote: ... PCR cleanup (NEB #T1030S), NEBuilder HiFi assembly (NEB #E2621S) ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... PCR cleanup (NEB #T1030S), assembly with the NEBuilder HiFi kit (NEB #E2621S) ...
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bioRxiv - Microbiology 2024Quote: ... colony PCRs (GOTAQ, NEB) were performed on positive E ...
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bioRxiv - Genetics 2022Quote: ... Two independent lines were successfully established and PCR validated through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). Primers for PCR were designed to flank the integration site (primer sequences ...
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bioRxiv - Microbiology 2020Quote: ... we designed primers for cloning into the pLEX-MCS vector using Gibson Assembly (NEB). To amplify ETV7 ...
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bioRxiv - Molecular Biology 2021Quote: ... indexed using NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S) and sequenced simultaneously using a Illumina HiSeq4000 System.
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bioRxiv - Biophysics 2021Quote: ... Primer extension was initiated by the addition of AMV reverse transcriptase (New England Biolabs) and allowed to proceed for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... the free primer was removed by Exonuclease I (80 U; M0293S, NEB, Ipswich, MA), and a 3’ DNA adaptor was ligated onto the cDNA product with T4 RNA Ligase 1 ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Neuroscience 2020Quote: ... generated template DNA by primer annealing with the NEBNext High-Fidelity polymerase (NEB, M0541S), and carried out in vitro transcription using the HiScribe T7 Kit (NEB ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: The primers were annealed and ligated with psiCHECK2 digested with XhoI and NotI (NEB). The pCDH vectors harboring single EBV miRNAs were used as described (Tagawa et al. ...
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bioRxiv - Genomics 2022Quote: ... NEBNext Multiplex Oligos for Illumina® Dual Index Primers (cat. #E7600, New England Biolabs), and AMPure XP Beads (cat ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was generated with oligo dT18 primer using the Protoscript II reverse transcriptase (NEB) in the presence of Murine RNase inhibitor (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis primers were designed using the “substitution” feature in NEBaseChanger v1.2.9 (New England Biolabs). Mutagenesis occurs at nucleotide 805-807 (GGA→ GAA ...
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bioRxiv - Immunology 2022Quote: ... The oligonucleotide was used with primer R1(RB202)* in a NEBuilder assembly reaction (NEB). The resulting plasmid was transformed in electro-competent E ...
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bioRxiv - Plant Biology 2020Quote: ... 1μL of each primer and 2U Phusion® High-Fidelity DNA Polymerase (NEB, USA) for 35 cycles (98 °C for 30s ...
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bioRxiv - Systems Biology 2019Quote: ... The RNA and free primers were degraded using 20U of Exonuclease I (NEB, M0293L) and 50U RNaseIf (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primers were ordered from IDT with Q5 Tms (calculated via the NEB Tm calculator) of 70-72 degrees ...
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bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...