Labshake search
Citations for New England Biolabs :
901 - 950 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... A different Unique Dual Index Primer Pair (NEB, E6440S) was added to each sample and 11 cycles were used for the PCR enrichment ...
-
bioRxiv - Neuroscience 2024Quote: ... with the primers constructed using the NEBuilder tool (NEB). Primers used for site-directed mutagenesis constructs are found in Table.1 ...
-
bioRxiv - Biochemistry 2023Quote: ... Primers were designed with NEBuilder (New England Biolabs, nebuilder.neb.com). Construct Sanger sequencing was performed by Eurofins (eurofinsgenomics.eu).
-
bioRxiv - Systems Biology 2023Quote: ... Excess primer is digested with Exonuclease I (NEB, #M0293L) at 37°C for 15 minutes.
-
bioRxiv - Microbiology 2023Quote: ... These primers were phosphorylated using T4 polynucleotide kinase (NEB) prior to PCR ...
-
bioRxiv - Systems Biology 2023Quote: ... using the NEBNext Universal i5 primer (New England Biolabs) and the N7 indices from the Nextera XT Index Kit v2 Set A (Illumina ...
-
bioRxiv - Cell Biology 2023Quote: ... using NEBNext Unique Dual Index Primers (New England Biolabs). Library preparation was conducted as per manufacturer protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Mutagenesis primers were generated using NEBaseChanger (New England Biolabs) and PCR conditions were determined using recommended annealing temperatures ...
-
VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... using the primers in (Table S1) by HiFi (NEB) cloning ...
-
bioRxiv - Microbiology 2024Quote: ... Unused primers were then degraded with Exonuclease I (NEB). The cDNA from different samples was then combined into pools ...
-
bioRxiv - Molecular Biology 2020Quote: ... from COLO205 cells was subjected to one-step RT-qPCR analysis using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...
-
bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
-
bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
-
bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... and the Luna One-Step RT-qPCR Kit (NEB) in accordance with the manufacturer’s protocol ...
-
A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... or Luna Universal One-Step RT-qPCR Kit (NEB) and 1.5 μl of reference primer/probe sets CDC-N1 (IDT 10006713 ...
-
Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila developmentbioRxiv - Molecular Biology 2019Quote: ... 3µg was reverse transcribed using Protoscript RT enzyme (NEB) as per manufacturer’s protocol and negative RT control was carried out similarly without RT enzyme ...
-
bioRxiv - Genetics 2024Quote: ... MultiV Reverse Transcriptase (RT) enzyme (New England BioLabs; NEB), 10x RT buffer (NEB ...
-
bioRxiv - Genetics 2024Quote: ... MultiV Reverse Transcriptase (RT) enzyme (New England BioLabs; NEB), 10x RT buffer (NEB ...
-
bioRxiv - Immunology 2022Quote: ... 1 μl 20X Luna WarmStart RT Enzyme Mix (NEB) and 10 μl 2X Luna Universal One-step Reaction mix (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... the LunaScript® RT Super Mix Kit from NEB was used according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RT reaction was performed using the Luna (NEB). The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and LunaScript® RT SuperMix (New England Biolabs, USA), referred to as Superscript™ and LunaScript® ...
-
bioRxiv - Cell Biology 2024Quote: ... or using NEB LunaScript RT SuperMix kit (NEB #E3010L). In both cases ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 2.5 U/µL M-MuLV RT (NEB #M0253L) unless indicated otherwise ...
-
bioRxiv - Genomics 2024Quote: ... 8 µL of 5X Induro RT Buffer (NEB M0681S), 12.6 µL nuclease free water ...
-
bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated using the NEBNext Small RNA Library Prep Sets (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the NEBNext small RNA Library Prep Set for Illumina (Multiplex Compatible) (New England Biolabs) was used to prepare samples according to recommended protocol ...
-
bioRxiv - Microbiology 2020Quote: ... libraries were prepared using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330), using manufacturer’s protocols ...
-
bioRxiv - Genomics 2022Quote: ... and IVT reactions were set up according to the HiScribe™ T7 (E2040S, NEB) or Sp6 (E2070S ...
-
bioRxiv - Genomics 2022Quote: ... and the NEBNext Small RNA Library Prep Set (New England Biolabs, Ipswich MA, USA), respectively ...
-
bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were set up using the Luna Universal qPCR Master Mix (NEB M3003S). Reactions included 6 µL of Luna Mastermix ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA fragmentation was performed using the Next Fast DNA Fragmentation & Library Prep Set (NEB). 100 ng purified DNA library was ligated with 2 μl barcode and 2 μl P1 (ThermoFisher) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Library indexing was done with NEBNex multiplex oligos for Illumina Set 1 (E7335S, NEB) and Set 2 (E7500S ...
-
bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
-
bioRxiv - Cell Biology 2024Quote: ... Gibson reactions were set up with NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), 50ng of linearized backbone ...
-
bioRxiv - Genomics 2024Quote: ... Small RNA libraries were made using NEBNext Small RNA Library Prep Set (NEB #E7330) with up to 5µg of RNA input ...
-
bioRxiv - Genetics 2019Quote: ... 80 pmol of oligo-dT18 primer (New England Biolabs, USA), 500μM of dNTP mix and 100U of M-MuLV reverse transcriptase (SuperScript III Reverse Transcriptase ...
-
bioRxiv - Bioengineering 2019Quote: ... unused primers are degraded with Exonuclease I (New England Biolabs), beads are washed and PCR amplified ...
-
bioRxiv - Biochemistry 2020Quote: ... The reformatting primers introduce XhoI and XbaI (New England Biolabs) restriction endonuclease sites which allow for in-frame cloning of the VHH sequence with the α-factor secretion signal and includes a vector independent 6xHis tag at the C-terminus ...
-
bioRxiv - Microbiology 2020Quote: ... Purified RNAs were random primed using Random Primer 9 (NEB) by incubation at 65°C for 5 minutes followed by rapid cooling on ice ...
-
bioRxiv - Microbiology 2022Quote: ... and 0.17 μL of random primers (3 mg/mL NEB) in a total volume of 5.25 μL ...
-
bioRxiv - Genomics 2022Quote: ... 900 nM primers and 250 unit/mL Eael (NEB, R0508S) in 1X ddPCR Supermix for Probes (no dUTP ...
-
bioRxiv - Microbiology 2020Quote: ... Primers (Integrated DNA Technologies) were designed with NEBuilder (www.nebuilder.com, NEB) to incorporate the appropriate 5’ ends necessary for Gibson Assembly and 3’ gene specific ends ...
-
bioRxiv - Molecular Biology 2020Quote: ... and reverse transcribed using random primer mix (New England Biolabs) and the Maxima Reverse Transcriptase kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... using Dual Index Primers (NEBNext Multiplex Oligos for Illumina, NEB). Libraries were sequenced on the NextSeq 500 platform (Illumina) ...
-
bioRxiv - Cell Biology 2022Quote: ... NEBNext Multiplex Oligos for Illumina Index Primers (New England Biolabs) were used for library preparation ...