Labshake search
Citations for New England Biolabs :
9201 - 9250 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... was used as per manufacturer protocol to isolate mRNA for input into the NEBNext® Ultra™ II RNA Library Prep Kit (New England Biolabs) using NEBNext Unique Dual Index Primers (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... The R119G and T82I point mutations were added to the parent AID* construct using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat # E0554S).
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Enlgand Biolabs, #E7760S/L).
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bioRxiv - Microbiology 2023Quote: ... cRNA synthesis was accomplished through in vitro transcription using the HiScribe T7 ARCA mRNA Kit with tailing (New England BioLabs, Cat. # E2060S).
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bioRxiv - Microbiology 2023Quote: ... we generated cDNA libraries from 100 ng of enriched mRNA using the NEBNext Ultra directional RNA library preparation kit and NEBNext dual index oligonucleotides for Illumina (New England BioLabs, Ipswich, MA). To ensure proper enrichment of bacterial transcripts we first sequenced the pooled library using a MiSeq Reagent Nano Kit v2 (300-cycles ...
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bioRxiv - Microbiology 2023Quote: ... The U6 promotor and the invariant scaffold are separated by a unique BsmBI restriction site using Q5® Site-Directed Mutagenesis Kit (NEB #E0554). Additional BsmBI restriction sites in the HIV-1 sequence were removed using Splicing by overhang extension (SOE ...
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bioRxiv - Immunology 2023Quote: ... The other pCMVtag2B vectors containing different truncated versions of rat ITCH (Fig 2F) were generated via Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Cat#E0554S) by circularizing the PCR products amplified with the primers listed in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The optimized for expression in PAO1 cell gene gp180 was synthesized by IDT and inserted into pHERDmCh using NEBuilder® HiFi DNA Assembly kit (New England Biolabs, UK).
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bioRxiv - Cancer Biology 2023Quote: ... DNAse-treated RNA in 5uL water was used as input to the NEB Ultra II directional library-preparation kit (New England BioLabs, MA, USA). If the concentration was less than this ...
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bioRxiv - Cancer Biology 2023Quote: 1 µg total RNA was used for NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Poly-A selection and cDNA synthesis were performed according to NEB protocol ...
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bioRxiv - Biophysics 2023Quote: ... was replaced with an HRV3C protease recognition site (LEVLFQGP) by site directed mutagenesis (Q5 site directed mutagenesis kit, New England Biolabs, Ipswich, MA). All amino acid substitutions were also introduced in GPΔTM and GPΔmuc via site- directed mutagenesis ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturer’s protocols and genomic libraries were prepared for Illumina sequencing using the NEBNext Ultra II DNA Library preparation kit (NEB #E7645, E7103) as described in the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... that was used to prepare RNA sequencing libraries with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NE Biolabs). Actinomycin D was used for first strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: Purified DNA was used to generate libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs cat# 7370) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA library preparation kit for Illumina (New England Biolabs GmbH, Frankfurt am Main, Germany). Libraries were sequenced to the depth of at least 60 million reads ...
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bioRxiv - Cancer Biology 2023Quote: ... 800 ng of RIN ≥7 of total RNA was used for library construction using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sheared to fragments of ∼300 bp and libraries were created using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA). An Illumina NextSeq 500 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... SCAR-seq libraries were prepared following the previous published protocol (11) without any modification except using NEBNext® Ultra™ II DNA library prep kit (NEB) for the end repair ...
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bioRxiv - Biochemistry 2023Quote: ... respective FoxP3 CDS with overhangs of pcDNA vector was synthesized by IDTDNA and then assembled with NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, #5520G). FoxP3 paralogs (FoxP1 ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-Seq libraries are prepared by Integragen SA (Evry, France) with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to supplier recommendations (NEB). The capture is then performed on cDNA libraries with the Twist Human Core Exome +Custom IntegraGen Enrichment System according to supplier recommendations (Twist Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... 36 sequencing libraries were obtained using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA), followed by PE 150 sequencing on the Illumina Novaseq 6000 ...
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bioRxiv - Microbiology 2024Quote: ... in vitro translation of the short-model mRNA was performed using a PURExpress® In VitroProtein Synthesis Kit (New England Biolabs, US). The reaction mixture (volume ...
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bioRxiv - Microbiology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The T7 PCR template was used to prepare dsRNA with the HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer protocols (New England BioLabs, MA, USA). For the injection experiments ...
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bioRxiv - Neuroscience 2022Quote: ... DNAse I treated RNAs were used to synthesize cDNAs using the ProtoScript cDNA First Strand cDNA Synthesis Kit (New England Biolabs Inc., USA). Primers for RT-PCR were designed to prevent amplification from genomic DNA (annealing sites in different exons or across intron-exon boundaries) ...
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bioRxiv - Neuroscience 2022Quote: ... Supplementary Table 4) from each sample was further processed with the Illumina NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S/L) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly(A)-enriched libraries for sequencing were then generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA). Paired-end sequencing was performed on the Illumina NextSeq instrument at the Harvard University Bauer Core ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of TET21-N cells (Control and 24 h doxycycline) was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK), from which complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... strand-specific RNA-seq libraries were constructed by using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ENO1-3’UTR and G6PD-CDS IDT PAGE purified oligos (Supplementary Table S6) containing T7 promoter were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040). RNA was purified using TRIzol (ThermoFisher,15596026 ...
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bioRxiv - Biochemistry 2022Quote: ... The resultant multiple overlapping DNA fragments were joined in a single isothermal reaction using a Gibson assembly cloning kit (New England Biolabs cat # E2611S). We designed our Gibson assembly primers to assemble the DNA fragments in the following order ...
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bioRxiv - Cancer Biology 2022Quote: ... Full length TEAD1 and TEAD2 coding sequences were amplified from pCMX-GAL4-TEAD1 and pCMX-GAL4-TEAD2 and cloned into the EcoRI site of pCDNA3.1 by Gibson assembly using the NEBuilder® HiFi DNA Assembly Kit (New England Biolabs Cat. #E2621). The TEAD3 gBlock fragment was similarly cloned into the EcoRI site of pCDNA3.1 by Gibson assembly ...
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bioRxiv - Cancer Biology 2022Quote: ... The CUT&RUN library was prepared using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645, E7710) as previously reported (Zhu et al ...
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bioRxiv - Immunology 2022Quote: ... and unc-54 3’utr pieces were Gibson assembled using the NEBuilder assembly kit and the assembled product was then PCR-amplified with Phusion DNA polymerase (New England Biolabs, Ipswich, MA). The PCR-amplified assembly was A-tailed for 30 min at 70 °C ...
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bioRxiv - Immunology 2022Quote: Plasmids containing a cDNA encoding the full-length M segment from SNV (pWRG/SN-M(opt) 67 and pWRG/AND-M(opt2) 68 were used mutagenized using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat# E0554S). Primers were designed using the NEBaseChanger tool (https://nebasechanger.neb.com/) ...
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bioRxiv - Genetics 2022Quote: ... the homology 5’arm and 3’arm was amplified and linked to the pBS backbone with Gibson Assembly Kit (NEB, cat. no.E2611L) as ‘pBS-CG32814-arm’ ...
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bioRxiv - Genetics 2022Quote: ... cDNA was produced from 100 ng of total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) with the d(T)23 VN primer ...
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bioRxiv - Genetics 2022Quote: ... approximately 50 ng of gDNA was used as input to the NEB Ultra II FS Library Prep Kit for Illumina (NEB cat. E7805L). Libraries were then sequenced using either paired end 2×36 bp or 2×72 bp read chemistry ...
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bioRxiv - Immunology 2022Quote: RNA from sorted Kmt2d-knockout and littermate control thymic cells was isolated by centrifugation to pellet and resuspended in 300 μL of Protection Buffer (Monarch RNA Isolation Kit, #T2010S; New England Biolabs Inc. (NEB), Ipswich ...
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bioRxiv - Immunology 2022Quote: ... as described by Corman et al84 were used alongside the Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswitch, MA) and CFX384 Touch Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Library preparation was performed using the NEB ultra DNA library preparation kit for Illmina (E7370) and Indexing primers (E7335S and E7500S) (New England Biolabs Inc, UK), following protocols described previously (Colles ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Immunology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies ...