Labshake search
Citations for New England Biolabs :
8451 - 8500 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: A plasmid encoding an eGFP-containing MARV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs). The vector backbone ...
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bioRxiv - Systems Biology 2022Quote: Library preparation was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries of ovule samples were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) using NEBNext® Multiplex Oligos for Illumina (Index Primers Set 1 and Index Primers Set 2 ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from total RNA and used for library construction with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: RNA libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... these fragments were then cloned into pEarleyGate 301[49] (predigested by XbaI and NcoI) using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). All clones were validated by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed using the NEBNext Ultra II DNA library preparation kit for Illumina according to the manufacturer’s instructions (NEB E7645S). To determine the number of PCR cycles required for amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomal-depleted RNA was then used for library preparation using NEBNext Ultra II Directional RNA Library Prep kit (NEW ENGLAND BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... is inserted in n-terminal of E2 protein of CHIKV 181/25 structural genes (C-E3-E2-6K-E1) and cloned into pcDNA3.1(+) expression vector using gibson assembly cloning kit (NEB, USA). The resulting plasmid is designated as ChAC-VLP ...
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bioRxiv - Microbiology 2022Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9 were performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the ELO genes were inserted using the primers described in Table 1 ...
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bioRxiv - Microbiology 2023Quote: RNA samples were depleted for ribosomal RNA using the NEBNext Bacteria rRNA Depletion Kit (New England Biolabs, Ipswich, Massachusetts, USA). Samples were pooled into a library using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and libraries for deep sequencing were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Quality of DNA libraries was validated using a 2100 Bioanalyzer Instrument (Agilent) ...
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bioRxiv - Plant Biology 2023Quote: ... and the purified mRNA was used to construct libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... A 5 µL aliquot was taken in which primers and dNTPs were then inactivated using Exo-CIP Rapid PCR Cleanup Kit (NEB). From the resulting mixture ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA of each template including the T7 promoter at the 5’ end was used following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis kit, NEB). Primers used are listed in Supplementary Table 1 ...
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bioRxiv - Zoology 2023Quote: ... Library concentration and quality were assessed with the Qubit Fluorometer and Bioanalyzer and molarity was estimated via qPCR with the NEBNext Library Quant Kit (New England Biolabs). Libraries were single indexed with NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... We converted the DNA into an Illumina sequencing library using the NEB Ultra II library preparation kit (NEB, Ipswich, MA) with a Y-adaptor ...
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bioRxiv - Genomics 2022Quote: ... The ploidy of the GM12878 and Detroit 532 were confirmed via NGS as follows: sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), and libraries were sequenced on a MiSeq (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina 96 reactions (New England Biolabs). Libraries were quantified using the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Microbiology 2022Quote: ... The LepB-CutFC-A and LepB-CutFΔC-ter versions were constructed by using the Q5 mutagenesis Kit (NEB Lab, MA) with cutF(CtoA)-F/ cutF(CtoA)-R and lepB-cutF(dC-ter)-F/ lepB-cutF(dC-ter)-R mutagenic primer pairs carrying the CxxxC to AxxxA and Δ10 amino acids from C-terminal proline-rich segment respectively (Table S2) ...
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bioRxiv - Genetics 2022Quote: Libraries for RNA-seq analysis were prepared using 500 ng of total RNA from three independent replicates using NEBNext Ultra II Directional RNA library prep kit for Illumina (NEB) and PolyA mRNA magnetic isolation module (NEB) ...
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bioRxiv - Genomics 2022Quote: ... and taken forward for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). A total of 4 libraries were generated ...
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bioRxiv - Genomics 2022Quote: ... adapters and the Sau3AI overhang adapters (375 pM) in a 40 μL solution using 50 units of Quick Ligase (Quick Ligation Kit; NEB) that was 1X reaction buffer ...
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bioRxiv - Genomics 2022Quote: ... and 100 ng of each PCR product was used to generate the final libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: Detection of viral genomes from heat-inactivated samples was performed by RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 specific primers targeting the N gene region (5′-TAATCAGACAAGGAACTGATTA-3′ and 5′-CGAAGGTGTGACTTCCATG-3′ ...
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bioRxiv - Microbiology 2022Quote: ... FastDigest restriction enzymes (Thermo-Fisher) were used for plasmid digestion and Gibson assembly was performed using the NEBuilder HiFi DNA assembly kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... On-bead PCR indexing-amplification was performed using custom-ordered indexing primers (IDT) matching the Illumina Nextera Index Kit sequences and 2x Phusion Master Mix (NEB). PCR reactions were amplified for 9-11 cycles ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Microbiology 2024Quote: ... Genes of interest were PCR amplified to include either their native or heterologous promoter and cloned using Gibson Assembly kit (NEB) into integration vectors pDG1662 (for insertion into the amyE locus) ...
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bioRxiv - Neuroscience 2022Quote: ... 250 ng of the purified PCR product was processed with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2022Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). DNA segments for Fc regions ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested RNA was prepared for sequencing with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB). Two biological replicates were performed for each condition ...
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bioRxiv - Neuroscience 2022Quote: ... Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNEXT poly(A) mRNA Isolation Module (New England Biolabs) according to manufacturer recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... Library preparation was performed using NEBNext® Ultra II Directional RNA Library Prep Kits for Illumina (New England Biolabs, USA), according to the manufacturer’s protocol with 8 PCR cycles ...
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bioRxiv - Molecular Biology 2022Quote: The DNase-treated RNA was used for cDNA synthesis utilizing the ProtoScript First Strand cDNA Synthesis Kit (NEB, Ipswich, MA) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription reactions were performed according to standard protocols provided with the high-yield in vitro transcription kits (E2040 and E2070, New England Biolabs), consisting of 40 mM rNTP (pH buffered with sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... the corresponding promoter sequences were inserted in the DNA templates using Q5 Site-Directed Mutagenesis Kit (E0554, New England Biolabs). DNA templates encoding for functional mRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Preparation of the sequencing libraries was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). 150-bp paired-end sequencing was carried out using Illumina Novaseq 6000 ...
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bioRxiv - Molecular Biology 2022Quote: The cDNA synthesis was performed as described before with a modified cleanup step using Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs) [26] ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Neuroscience 2022Quote: ... The N533H and L534I mutations in the pEGFP-SFPQ and pGEX6P1-SFPQ-276–598 constructs were generated with the Q5 site-directed mutagenesis kit (New England Biolabs). All constructs were verified by DNA sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 ng of bending site RNA samples from five-day-old Col-0 and myb93 plants using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs) following manufacturer protocols ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... Plasmids were then digested with BamHI and NotI and the deletion fragment was gel extracted (Monarch DNA gel extraction kit, NEB). 100ng of purified deletion fragment was then added to competent S ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared from sheared gDNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the concentration was measured by qPCR using a NEBNext Library Quant Kit for Illumina (New England BioLabs GmbH, Hessen, Germany), and the size was determined by Bioanalyzer 2100 using Agilent High Sensitivity DNA Kit (Agilent Technologies GmbH ...
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bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...