Labshake search
Citations for New England Biolabs :
8351 - 8400 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 25ng of DNA was used to prepare sequencing libraries using NEBNext® Ultra™ DNA Library Prep Kit (NEB, E7370L) according to manufactures manual ...
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bioRxiv - Genomics 2021Quote: All samples that met the previous criteria were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The poly(A) RNA library was obtained using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) including the NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: The pCeTyX Ty1hygro-AI carrying plasmid was constructed by assembling four overlapping DNA fragments thanks to the Gibson Assembly Cloning Kit (NEB). The 5,472 bp plasmid backbone was obtained after restriction of p41Neo 1-F GW (https://www.addgene.org/58545/ ...
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bioRxiv - Genomics 2020Quote: ... and 150ng of each sample were fed to library preparation with NEBNext Ultra II DNA Library Preparation kit for Illumina (New England BioLabs) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) and then sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 1 kb of upstream and downstream DNA were cloned into the suicide vector pK18mobsacB by Gibson assembly45 using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The vector was introduced into B ...
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bioRxiv - Cell Biology 2021Quote: ... using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers) and cloned by Gibson Assembly Cloning Kit (EE5510S, NEB). All primers were designed using SnapGene (GSL Biotech LLC ...
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bioRxiv - Biochemistry 2020Quote: ... Mature tRNA sequences were obtained by deleting the intron sequence using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). All constructs in this study were verified by Sanger sequencing.
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bioRxiv - Genomics 2020Quote: ... we prepared libraries of circle-enriched and whole genomic control samples using NEBNext FS DNA Ultra II Library Prep Kit (New England Biolabs) using protocol modifications outlined in (Sproul and Maddison 2017) ...
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bioRxiv - Molecular Biology 2021Quote: ... The corresponding non-binding mutant YESubB (S31A; Y100F) was engineered from the wt template above using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) and sequence verified.
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bioRxiv - Molecular Biology 2019Quote: ... This was assembled with PfoI-linearized pRS426 using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S).
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bioRxiv - Molecular Biology 2022Quote: The pre-crRNA was transcribed in vitro using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEW ENGLAND BioLabs). Transcription template (dsDNA ...
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bioRxiv - Microbiology 2022Quote: ... PCRs purified using the Nucleospin gel and PCR clean-up kit were cloned into plasmid backbones digested with restriction enzymes (NEB) using the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: Libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina® (NEB #7760L) with 2.5 ng RNA input (1/300 adaptor dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... HDR plasmid with mutant me31B genes was generated by mutating the me31B wild type gene in the wildtype HDR donor plasmid by using the Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s recommended protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... 5ng of DNA was used for library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S), SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal RNA was depleted from 300 ng of the total RNA samples using the NEBNext rRNA Depletion Kit (NEB, E6310L), then processed for RNA-seq library prep with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... enzymatic shearing was performed on 500ng of genomic bacterial DNA using the NEBNext Fast DNA Library Prep kit (New Englands Biolabs) and following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAseq libraries were created from 250ng of total RNA using the NEBNext UltraII Directional RNA Library Prep kit (New England Biolabs) using half volume reagents and 14 cycles of PCR ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were multiplexed using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was purified using standard Phenol:chloroform:isoamyl alcohol extraction and ethanol precipitation and used for library preparation with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 9 (H3K4me1 ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... The Hi-C library for Illumina sequencing was prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... mRNA sequencing libraries were prepared with 500 ng of total RNA of each sample using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were verified by agarose gel electrophoresis and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... and BRD4L-MU34 PAS reporters were generated by site-directed mutagenesis of TRIM9S-WT or BRD4L-WT PAS reporters using Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Bioengineering 2022Quote: ... HiScribe T7 RNA polymerase kits and Q5 2x HiFidelity PCR mastermixes were purchased from New England Biolabs (NEB, Ipswitch, MA). Sodium cacodylate solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library preparation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L) and eluted with 20 μl of ddH2O ...
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bioRxiv - Biochemistry 2022Quote: MBOAT7 mutants were generated by site-directed mutagenesis on the pFBM construct expressing MBOAT7 with a C-terminal GFP and strep tag using the Q5 Mutagenesis Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra RNA Library preparation kit (New England Biolabs). First ...
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bioRxiv - Biochemistry 2022Quote: ... DED1 Trp-to-Ala point mutations were introduced using the Q5 site-directed mutagenesis kit (NEB, Frankfurt am Main, Germany) with primers carrying the sequence variation and verified by sequencing ...
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bioRxiv - Biophysics 2022Quote: ... vulnificus adenine riboswitch aptamer domain by in vitro transcription of the corresponding DNA oligonucleotides (HiScribe T7 High Yield RNA Synthesis Kit, New England Biolabs; oligonucleotides ordered from Eurofins) ...
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bioRxiv - Biophysics 2022Quote: ... a site-specific insertion of cysteine at position 287 was achieved in the long L4 loop of Mb4-tFhuA by site-directed mutagenesis (Q5 mutagenesis kit, New England Biolabs). This cysteine-containing Mb4-tFhuA was expressed and purified as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... NGS libraries were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). Ten cycles of PCR amplification were applied to all libraries ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions between 100–400 nt were cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). Multiplexed samples were submitted to the IMG Genomics and Bioinformatics facility for library synthesis using the NextSeq® 500/550 High Output Kit v2 ...
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bioRxiv - Biochemistry 2022Quote: Site-directed mutagenesis was performed in order to generate all the point mutants and variants described in this study using the Q5-site directed mutagenesis kit (New England Biolabs) following manufacturer instructions and the indicated primers (see extended material and methods).
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bioRxiv - Biochemistry 2022Quote: ... a tolerant position was mutated to facilitate making a labeled LexA variant.56 The His-LexA W201C mutant was generated using a Q5 Site-Directed Mutagenesis Kit (NEB) and the mutation was confirmed via sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... Synthetic ORF1a target RNA fragment was produced from the corresponding cDNA fragment by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified and cleaned using a Monarch® PCR & DNA cleanup kit (New England BioLabs, Ipswich, MA). RNAs for the in vitro test were transcribed using a HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs ...
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bioRxiv - Biochemistry 2022Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and adenylated using 5′ DNA adenylation kit at the 5′ end (NEB). To reduce a ligation bias ...
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bioRxiv - Biochemistry 2022Quote: ... and luciferase DNA fragments were cloned into pUC18 at the same time by using NEBuilder HiFi DNA Assembly Cloning Kit (M5520, NEB) to form the substrate plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... and C-terminal truncations (-57Q, -56IQ, -55DIQ and -54DDIQ) were performed on phyg-C-CSNAP-cerulean using the Q5 mutagenesis kit (NEB). HAP1 WT cells were transfected with 5 μg plasmid DNA using JetPRIME (Polyplus) ...
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bioRxiv - Cancer Biology 2022Quote: ... WES and RNA sequencing from the PDX samples was performed using NEBNext Ultra II FS DNA library Kit for Illumina (New England Biolabs), SureSelectXT HS Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library For Illumina Multiplexed Sequencing Platforms (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: A range of 5 to 30 ng of cfDNA was used to generate WMS libraries with NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) per manufacturer instructions.