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Citations for New England Biolabs :
801 - 850 of 888 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: 8.3 μM bdSUMO-HSPB611–20 fusion protein containing pSer or nhpSer at site S16 of HSPB6 were reacted with 3 units of λ phosphatase (NEB) according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... The PCR was carried out with 1 μl of Lambda DNA (20 ng/μl; Catalog number: N3011S. Vendor: New England Biolabs Inc), 12.5 μl of KAPA 2X Master Mix ...
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bioRxiv - Microbiology 2021Quote: SYBR-based qPCR reactions were set up in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1X HF Buffer ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL of the IEC fraction was concentrated for 4 h in vacuum to which 20 μL of the dried sample and 2 μL of 10xGlyco Buffer (New England Biolabs™) were added into 2 mL Eppendorf tube and incubated in the thermomixer at 99 °C for 10 min ...
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A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastbioRxiv - Genomics 2020Quote: Half of one plasmid extraction was used to prepare a 25 μl digestion in 1x final concentration CutSmart buffer with 20 U XhoI (NEB R0146L) and incubated 1 hour at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... First-round Ig-encoding sequence amplification (20 cycles) was performed using Q5 High-Fidelity Master Mix (New England BioLabs, Ipswich, MA) and nested gene-specific primers ...
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bioRxiv - Immunology 2020Quote: ... For genotyping hair samples were incubated in 250 μL of Direct PCR reagent and 20 mg/mL Proteinase K (New England Biolabs, P8102) for 1 h at 55°C ...
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bioRxiv - Genomics 2021Quote: ... in a 20 μl reaction mixture consisting of Luna® Universal qPCR Master Mix (New England Biolabs, Inc., Cat. No.: NC1276266), forward and reverse primer pairs (F515-GTGCCAGCMGCCGCGGTAA and R806-GGACTACHVGGGTWTCTAAT ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... and incubated for 20 min at 37°C in the presence of 1,000 gel units of micrococcal nuclease (catalog number. M0247S; NEB, Ipswich, MA) in a 500 ul volume of buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... RNase-free DNase Buffer was added to samples until 1× final concentration together with 20 units of DNase I (NEB, M0303L) and incubated at 37°C for 20 minutes ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 20 µl of distilled water and mixed with 18 µl gel loading dye (New England Biolabs, Inc.). Samples were resolved in a 10% SDS-polyacrylamide gel ...
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bioRxiv - Genomics 2023Quote: ... then the linearized backbone and the annealed duplex were ligated at room temperature for 20 minutes using the Blunt/TA Ligase Master Mix (NEB M0367S). Transformation ...
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bioRxiv - Molecular Biology 2023Quote: ... The plugs were washed in 20 ml of Milli-Q water for 30 minutes and then 10 ml of 1x rCutSmart buffer (New England Biolabs, B6004S) for 1 hour followed by a further hour in 5 ml of 1x rCutSmart buffer ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was amplified with a first set of oligos in a PCR reaction on a qPCR machine to detect amplification in real time to capture linear amplification (0.75 μl of 20 mM P3tall and P6tall oligo mix, 15 μl of 2x NEB Next (M0541L), 14 μl cDNA ...
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bioRxiv - Genomics 2024Quote: ... Samples were then washed twice with PBS-T and incubated for 15 minutes with 20 µg/mL Streptavidin (New England Biolabs N7021S) and 100 µg/mL rAlbumin (New England Biolabs B9200S ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... pET30a-SEP vector was stored at -20°C prior to transform BL21 (DE3) competent bacteria (New England Biolabs, Évry-Courcouronnes, France).
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bioRxiv - Systems Biology 2023Quote: ... five reactions each with 250 fmol of the construct carrying the mutant fragment or the original wild-type pNES-EGFP-C1-PH-ARNO(I303E)x2 construct were digested with 20 U each of BamHI-HF (NEB #R3136S) and HindIII-HF (NEB #R3104S) ...
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bioRxiv - Molecular Biology 2023Quote: ... The specimens were then incubated with a 20-fold diluted Quick Ligase in 1x Quick Ligase Reaction Buffer from Quick Ligation Kit (New England Biolabs, M2200), supplemented with an additional 1 mM ATP (TAKARA Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue samples were stained using Vizgen’s Cell Boundary Kit (Vizgen, 10400009) and blocked in blocking solution (Vizgen, 20300012) supplemented with a 1:20 dilution of Rnase inhibitor (NEB, M0314L) for one hour ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... DNA fragments were amplified with 15-20 bp of homology regions in primers using Phusion R High Fidelity DNA polymerase (New England Biolabs; NEB) and homology based assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Every marker was amplified in 20 μl of a reaction mix containing a 1X Standart Taq Reaction Buffer (M0273E, New England BioLabs Inc.), 0.4 μl of 10 mM dNTP ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of a 1:250 dilution of the annealed oligos were ligated to 50 ng of dephosphorylated BsaI-digested vector at 16°C overnight in 20 μl final volume containing 1x T4 DNA ligation buffer (NEB, B0202S), and 400U T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated at 37°C for 20 min followed by dephosphorylation with 1 U Shrimp Alkaline Phosphatase (rSAP, New England Biolabs, #M0371S) and incubation for another 20 min ...
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bioRxiv - Genomics 2024Quote: ... 480 ng of the sgRNA-barcode library and 230 ng of the reporter construct were mixed in separate 20 µL NEBuilder HiFi assembly reactions (NEB, E2621S) for 1 h at 50 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Neuroscience 2024Quote: ... 12 20-μL reactions of gibson assembly were performed for 1 hour at 50C using the HiFi DNA Assembly MasterMix (NEB, E2621), with each reaction containing 184.9 ng of SacII-digested plasmid and a 10-fold molar excess of PCR-amplified insert ...
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bioRxiv - Molecular Biology 2024Quote: ... A modified p5 adapter was ligated onto A-tailed break-ends in-situ using T4 DNA ligase (16-20 hr 16°C; NEB, M0202M). Excess adapter was removed with 10 washes with wash buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... and pSwi6S18/24A were incubated for 20 minutes at 37°C with 50 U of Calf Intestinal Phosphatase (QuickCIP, NEB, M0525S) in 100 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared from 20 uL of ChIP DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Library molarity and quality were assessed by Qubit and Tapestation (DNA High sensitivity chip) ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Microbiology 2024Quote: ... N-4005, and N-4004) were used to cap available 3’-terminal ends using terminal transferase (1 µl, 20 units) (NEB #M0315S), TdT reaction buffer (1.5 µl) ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... 200 ng of purified product served as template in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040), fully substituting UTP with N1-methylpseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed in a 20 μL total volume reaction using the Q5 high fidelity DNA polymerase (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were then ligated to pre-annealed Nano-tRNAseq 5’ and 3’ RNA adapters at room temperature for 2 hours with 20% PEG PEG 8000 (NEB, B10048) using recombinant E ...
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bioRxiv - Microbiology 2020Quote: ... all three fragments had 20 base pair overlap sequences and were assembled by NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). All constructs were verified by Sanger sequencing prior to transformation into the B ...
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bioRxiv - Developmental Biology 2020Quote: ... eluted in 20 μl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of P5_BRB primer (5 μM ...
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bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
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bioRxiv - Systems Biology 2021Quote: ... and then cloned into the EagI and EcoRI sites of the plasmid pJK03 with multiple 20 microliter ligation reactions (NEB T4 ligase). The libraries were transformed into 5-alpha electrocompetent cells (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The 1970 bp DNA was then gel purified and 500 ng of DNA template was loaded into a 20-μL in vitro transcription reaction with 5,000 units of T7 RNA polymerase (New England BioLabs, product number M0251LVIAL), 1X RNAPol Reaction Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed in a final volume of 20 μl including NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (both New England Biolabs, Herts, UK) and 5 μl of RNA sample ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Microbiology 2023Quote: ... Radiolabeling of 50 pmol of RNA substrate was performed in 20 μl of reaction mix using 10 U of T4 PNK (New England Biolabs, cat. M0201) and 3μL of ?32P-ATP (Perkin Elmer ...
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bioRxiv - Systems Biology 2023Quote: ... The Gibson assembly was performed using 34 fmol of backbone and 68 fmol of gene fragment in 20 μl reactions (New England Biolabs, Ipswitch, MA). The reactions were incubated for 15 min at 50 °C ...
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bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna® Universal qPCR Master Mix (New England Biolabs, Frankfurt, Germany). Each primer was added at a final concentration of 300 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were digested in a 20 μl reaction with NEB Cutsmart buffer for 30 minutes - 1 hour with SmaI (NEB, Cat# R0141) to assess rDNA promoter methylation or with HpaII (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... Up to 10 ng of gDNA was amplified in a 20 μl volume using NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) supplemented with 5% dimethyl sulfoxide and barcoded primers specific to HTT Exon 1 (0.5 μM each) ...