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Citations for New England Biolabs :
501 - 550 of 888 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The ligation mix (20 μl) was added to a 50 μl reaction containing 1X thermopol buffer (NEB), 200 μM each of dATP ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed in 20 µL reactions using Luna qPCR Universal qPCR Master Mix (New England Biolabs), 5 ng of cDNA as template ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then amplified by performing 20 cycles of PCR using Q5® High-Fidelity 2X Master Mix (NEB) (final concentration between 30-100 ng.µl-1) ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 20 µL H2O.Preparation of sequencing libraries was performed using NEBNext Ultra II kit (NEB) with 7 PCR cycles for library amplification ...
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bioRxiv - Bioengineering 2023Quote: ... or with 20 U each of KpnI-HF and SpeI-HF (New England Biolabs, R3142 and R3133) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 20 uL of sheared DNA was then digested with 1 U of DpnI in cutsmart buffer (NEB) for 3 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... 0.5 % Tween-20 and the selected genes amplified by PCR using Q5® Hot Start polymerase (NEB) using forward primer ...
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bioRxiv - Physiology 2022Quote: ... a fresh aliquot of sgRNA and Cas9 enzyme (Cas9 Nuclease, S. pyogenes, 20 μM; NEB, Ipswich, USA) was used for each day and stored on ice (find respective concentrations in suppl ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Each reaction was initiated by the addition of 20 μM nt ΦX174 RF1 DNA (New England Biolabs) previously digested by PstI ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of purified PCR product was used in a 20 µl HiFi assembly reaction (NEB, E2621S). HiFi assembly was performed at 50°C for 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... then combined and treated with 1% SDS and 20 µg of proteinase K (New England Biolabs, #P8107S). The reaction was incubated for 1 h at 55°C ...
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bioRxiv - Immunology 2023Quote: Cleared cell lysates were incubated with PNGase F (500–1,000 U per 20 μl of lysats, NEB) for 30–45 min at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 20 pmoles of dephosphorylated RNA was combined with 2 μl 10 x T4 Polynucleotide Kinase buffer (NEB), 3 μl ATP(y-32P ...
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bioRxiv - Neuroscience 2024Quote: ... tube 2: 20 µL VLP suspension + 6 µL water + 1 µL Proteinase K (200 µg/mL, NEB), and tube 3 ...
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bioRxiv - Biochemistry 2024Quote: ... TdT (20 U) and α-32P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... MNase (non-specific DNA digestion) used MNase buffer and 2 μL of enzyme (20 units/μL) PvuII (NEB), AluI (NEB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 40 µl of filtered double-distilled water and 20 µl of blue SDS sample buffer (New England Biolabs) were added to the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μM of linear dsDNA PhiX174 replicative form I (Phix174 RFI homologous plasmid purchased from New England Biolabs and linearized by digestion with the restriction enzyme XhoI or PstI followed by phenol-chloroform extraction ...
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bioRxiv - Systems Biology 2020Quote: ... DNA assembly reactions using 0.4 pmol insert and 0.1 pmol vector per 20 μL HiFi Master Mix (New England Biolabs) were incubated at 50°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs, Germany) and the luciferase activity was determined in a ClarioStar Plus plate reader (BMG Labtech ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA templates were amplified over 20 cycles using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Inc.). The final concentration of components in a 100 µl PCR reaction was 1ng/µl of DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl tagmentation product was mixed with 20.4 μl Q5 High-Fidelity 2X Master Mix (NEB, Cat.No. M0492L), 0.4 μl SuperScript II reverse transcriptase ...
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bioRxiv - Evolutionary Biology 2022Quote: ... End repair was performed by adding 20 μl of the End Repair Mix (7 μl of 10x NEB ligation buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 40 μl of filtered double-distilled water and 20 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5μL of 20% Triton X-100, 1μL BSA (100x, 10mg/mL) and 10μL HaeIII (10U/μL, NEB, R0108S) with shaking at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... DNase I de-staining buffer (PBS containing 1X DNase buffer and 20 units of DNase I (NEB, #M0303) in 500ul) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was transferred to a new tube and incubated with DNA Digestion Buffer (20 μl DNaseI (NEB M0303), 0.5 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... pWPI Puro was digested by PmeI and dephosphorylated using 20 U of calf alkaline phosphatase (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... at final volume 20 μL with 10 μL of Luna Universal qPCR Master Mix (New England Biolabs, Germany). The concentration of the primers varied from 0.2-0.5 μM (for further details regarding genes ...
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bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2% Tween-20) and RNA 5’ ends where labelled with [γ-32P]-ATP and T4 PNK (NEB, M0201L) at 37°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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bioRxiv - Genomics 2023Quote: ... and were ligated at room temperature for 20 minutes using the Blunt/TA Ligase Master Mix (NEB M0367S). Transformation ...
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bioRxiv - Neuroscience 2022Quote: ... 4 and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302). Digestion products were resolved on 8% acrylamide/bis TBE gel and visualized with EtBr.
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of total DNA was first digested overnight with 20 U of SmaI (New England Biolabs, R0141) at 25 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted RNA was resuspended in 20 µL nuclease-free water and treated with DNase I (New England BioLabs) for 10 minutes at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... first with 20 pmol membrane impermeable SNAP dye (10 μM SNAP-Surface® 488, New England BioLabs Inc.) followed by 20 pmol membrane permeable SNAP dye (10 μM SNAP-Cell® 647-SiR ...
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bioRxiv - Genetics 2024Quote: ... Cell pellets were resuspended in 1.2 mL lysis buffer and incubated with 20 μg/mL of lysozyme (NEB) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were carried out in 20 μl reaction solutions containing 1X Luna® Universal qPCR Master Mix (NEB), 20 ng cDNA and 0.25 μM each gene-specific primer ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in NEB buffer prior to centrifugation at 11,500 rpm for 20 minutes at 4°C on a sucrose gradient (1.6M sucrose in NEB and 0.8M sucrose in NEB). Nuclei-containing pellets were fixed with 0.3% formaldehyde in NEB and rotated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... dried K-ε-GG peptides were resuspended in 20 µl of 1x CutSmart buffer (New England Biolabs, B7204S) and incubated with 2.5 u of QuickCIP phosphatase (New England Biolabs ...