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Citations for New England Biolabs :
651 - 700 of 888 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... We performed assays for the E and RNase P genes separately in 20 μL reaction volumes using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs). The final concentrations of primer and probe were 400 and 200 nM ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the heads of 20 male adults or 17 female adults using a Monarch Total RNA Miniprep Kit (NEB). 3 replicates for male and 3 replicates for female flies were done for each genotype ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 20 uL 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and using touchdown PCR cycling at 98°C for 30s ...
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bioRxiv - Neuroscience 2021Quote: ... using auto-induction protocols (Studier, 2005) at 16 °C for 20 h or in BL21 pLysY (New England Biolabs, C3010I) for isotopically labelled proteins with IPTG induction for 16 h at 20 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μL of the diluted PCR product was amplified in a 20 μL barcoding reaction that included 1X NEBNext Phusion Master (NEB) and 4 μL of the Fluidigm Access Array barcoding primers ...
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bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
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bioRxiv - Neuroscience 2020Quote: ... each round of evolution also included a library created by gene-shuffling the selected clones using the staggered extension PCR method [20] and Taq polymerase (New England Biolabs). The amplified DNA fragments were inserted into the constitutive bacterial expression vector pNCS (gift from Nathan Shaner ...
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bioRxiv - Plant Biology 2020Quote: First amplification was done in 20 μL with DegPstI (degenerated primers) and Rb1 (on the aphVIII cassette) primers with Taq polymerase (NEB) using 58°C annealing temperature for 5 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-protein complexes were radioactively labeled with 32P-γ-ATP (20 μCi) using 40U T4 Polynucleotide Kinase (New England Biolabs) in supplied PNK buffer for 30 min at 37ºC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40 μl of filtered double-distilled water and 20 or 30 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads or the inputs ...
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bioRxiv - Molecular Biology 2022Quote: ... and mixed with a master mix consisting of 20 μl of 2 x NEBNext Ultra II Q5 Master Mix (NEB), 1 μl of 40 μM P5* primer ...
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bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The L5 RNA linker at 1 μM concentration (Table S1) was ligated to RNA in a 20 μl reaction using by 1 U/ μl of RNA Ligase 1 (NEB) in the presence of 15% PEG8000 ...
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bioRxiv - Microbiology 2022Quote: ... Individual fragments were assembled by Gibson assembly at 50°C for 1 h in a total reaction volume of 20 µl (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs), thereby adding 6x His-tag coding sequences to the 3’ end of ply007 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then diluted to 20 ng/uL and 20-60 ng of RNA was used for qPCR using Luna universal one-step RT-qPCR kit (NEB) as per manufacture’s instructions ...
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bioRxiv - Biochemistry 2022Quote: SNAP–tagged kinesin-8B-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... 5μg genomic DNA of each tumor was used as template in a pre-amplification reaction using unique barcoded primer combination for each tumor with 20 cycles and Q5 High-Fidelity DNA Polymerase (NEB). The following primers were used:
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1 μg (20 ng/μl) of high-quality total RNA (extracted using Monarch Total RNA Miniprep Kit, NEB) was supplied for sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... captured messenger RNA was isolated from the lysate by adding 20 μl of LBB-washed streptavidin-coated magnetic beads (New England Biolabs) and incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and following the KLD enzyme step the product was heated to 65°C for 20 minutes and then double digested with PseI and FseI in CutSmart buffer (NEB) for 60 minutes at 37°C to remove any remaining plasmid that was not eliminated by the DpnI in the KLD step ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNAs were diluted 1:20 and then qPCR was carried out in triplicate using the Luna Universal qPCR Master Mix (New England Biolabs). 3 primer sets targeted different exon-exon junctions in the lmo7a coding region and primers targeting rps13a as the control housekeeping gene were used for normalization ...
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bioRxiv - Molecular Biology 2020Quote: ... End-repair reaction was cleaned using 2X Agencourt AMPure XP beads and eluted in 16.5 μl of EB that was used for A-tailing reaction in 20 μl of NEBNext dA-Tailing reaction buffer (NEB) with 7.5 U of Klenow fragment exo-(NEB ...
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bioRxiv - Microbiology 2021Quote: ... The 37-mer (50 μM) and the 14-mer (20 μM) RNAs were mixed with Vaccinia Capping Enzyme buffer (NEB), 2 mM SAM ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were ligated to barcode primers 1-20 of NEBNext Index Primers for Illumina sets 1 and 2 (New England Biolabs) and libraries analysed using DNA High Sensitivity chip on an Agilent 2100 Bioanalyzer before being pooled ...
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bioRxiv - Microbiology 2020Quote: ... 20 μg of recombinant PbChiB2 and PbChiB4 resuspended in 20 mM Tris (pH 8.0) were independently mixed with 2 mg of chitin magnetic beads (NEB, CAD), chitin from shrimp shell (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Designed fragments were PCR-amplified from purified phage A006 or synthetic DNA to yield a total of six DNA fragments per phage genome followed by Gibson assembly at 50°C for 1 h in a total reaction volume of 20 µl (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs). Assembly reactions were carried out with purified DNA fragments to yield synthetic genomes ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of MBP-SYCP1N at 2 mg/ml was incubated for 20 minutes on ice with 100 ul amylose resin slurry (NEB) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Genetics 2020Quote: ... A 1000 pmol sample of PAGE-purified sgRNA was treated with 20 U of calf intestine phosphatase (M0525L; New England BioLabs) at 37°C for 3 h to remove the 5’ phosphate group to prevent triggering innate immune responses(35) ...
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bioRxiv - Cell Biology 2020Quote: ... The gene fusion was then amplified using primers 20 and 21 and cloned into pClimDC linearized at the SalI restriction site using Gibson Assembly (New England Biolabs). To build pClimDC-Lifeact:mCh ...
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bioRxiv - Microbiology 2020Quote: ... The PCR was performed in a final volume of 20 μL: 10 μL of Q5 polymerase master mix (New England Biolabs), 0.5 μL of forward primer 10 uM ...
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bioRxiv - Biochemistry 2020Quote: ... Hartmann Analytic) for 30 min at 37°C in 50 µl volumes with 20 units of T4 PNK (New England Biolabs) in the provided reaction buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... One μl of the total cDNA was used for the PCR (20 μl volume) performed with Taq DNA polymerase (New England BioLabs) and primers (Table 2) ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl cDNA reaction mix was amplified by adding 25 μl of Long Amp Taq 2x Master Mix (NEB-kit), 1.25 μl SR primer for Illumina (NEB-kit) ...
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bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 μl contained 10 μl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
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bioRxiv - Cancer Biology 2020Quote: ... was stored at 20° C or amplified immediately in 50 μl reactions with high-fidelity 2X PCR Master Mix (New England Biolabs) using a common forward primer and different reverse primers with unique barcodes for each sample ...
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bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
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bioRxiv - Genomics 2021Quote: ... “Fuorf_RNA_adapter” was ligated to the 3’ end of 10 μl of cDNA in a 20 μl reaction at 65 °C for 1 hour using Thermostable 5’ App DNA/RNA ligase (New England Biolabs). The reaction was inactivated at 95 °C for 3 minutes and 2 μl of the reaction was used as a template for PCR for sequencing library generation as described below.
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bioRxiv - Evolutionary Biology 2021Quote: ... Flanking primers contain 20 bp of 5’ and 3’ overlap with the pcDNA3.1 vector for Gibson Assembly (New England Biolabs, #E5510). The resulting library had an estimated complexity of 322 = 1024 codon variants and 202 = 400 amino acid variants ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
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bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 µl contained 10 µl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... An appropriate volume of reaction mastermix was prepared with the following composition: 20% v/v BsaI (New England BioLabs R3733S) or BbsI (Thermo Scientific ER1012) ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 50 unit of T4 DNA ligase along with 7 μl of 20 ng/μl of BSA (Biolabs) and 7 μl of 100 mM ATP were added to reach a final volume of 700μl ...
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bioRxiv - Biochemistry 2022Quote: ... The positive clones were identified by colony PCR in a 20 μL reaction using Taq DNA polymerase with ThermoPol buffer (New England Biolabs) with PCR parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteinase K was inactivated (20 min 82 °C) and the crRNA target site was amplified with One Taq Hot Start DNA polymerase kit (NEB), followed by Sanger sequencing (primers in Table S3) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 10 min and 2 μL of 20 mg/ml proteinase K (New England Biolabs, Cat. #P8107S) were added ...
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bioRxiv - Genetics 2023Quote: ... 2 μL of genomic DNA prep was fragmented by restriction digest in 20 μL reactions with EcoRV-HF and HinDIII-HF (New England Biolabs) for 2h ...
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bioRxiv - Molecular Biology 2023Quote: ... were manipulated in a final volume of 20 μL labeling mixture containing 1X T4 polynucleotide kinase (PNK) enzyme buffer (NEB), milli-Q water ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1% NP-40 buffer as above and 20 µg of lysate or 50 µL of supernatants were subjected to Endo H (P0702S NEB) or PNGase F (P0709S NEB ...