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Citations for New England Biolabs :
701 - 750 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 500ng RNA using a Multiscribe High Capacity cDNA Synthesis kit (Thermo) and qPCR was performed using Luna qPCR Master Mix (New England Biolabs) against primers listed in table 2.
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bioRxiv - Physiology 2023Quote: ... Afterwards the mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCR Master Mix, M3003E, New England Biolabs) on a VIA7 Real Time PCR System (4453534 ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR was performed on 10 ng total RNA using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L). bin3 ...
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bioRxiv - Bioengineering 2023Quote: RT-qPCR was carried out from the isolated RNA using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The Ct values of samples were determined by quantitative PCR (qPCR) using a Luna Universal Probe One-Step RT-qPCR Kit (cat. #E3006, New England Biolabs) with LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:10 and used for qPCR reactions employing Luna® Universal qPCR Master Mix (New England Biolabs). Ct values were computed by the instrument and used for calculation of ΔΔCt using ACTB (Beta-actin ...
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bioRxiv - Microbiology 2024Quote: ... Diluted supernatants were used as the template DNA (5 μL) for qPCR using a 2×Luna universal qPCR master mix (New England BioLabs), with 0.5 μM of primers ...
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bioRxiv - Immunology 2024Quote: ... Gene expression levels were quantified by reverse transcription quantitative PCR (RT-qPCR) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and being conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... equal volumes of cDNA were used for qPCR using the Luna® Universal qPCR Master Mix kit (New England Biolabs). For qPCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... and used as the template for the SYBR Green-based qPCR reaction using the Luna Universal qPCR Master Mix (NEB). The reaction and analysis were conducted using an ABI Step One real-time thermocycler (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Both first-strand complementary DNA (cDNA) synthesis and RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in 384-well plates using a QuantStudio 7 Flex (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA was then diluted 1:4 to a total volume of 20 μL and 1 μL was used as template for qPCR with the Luna qPCR Master Mix (New England Biolabs) in a total reaction volume of 10 μL ...
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bioRxiv - Biochemistry 2024Quote: ... DNA pellet was resuspended in 50 μL of TE buffer and 1 μL was used in qPCR reaction with Luna qPCR Master Mix (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... Luna Universal qPCR Master Mix (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using a Luna Universal qPCR Master Mix (NEB) at the fast setting following the manufacturer’s instructions to determine dCT levels in triplicate ...
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bioRxiv - Immunology 2022Quote: ... Luna Universal qPCR Master Mix (New England Biolabs) was used for quantification of fungal ITS2 sequences and TaqMan Gene Expression Assays (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: The Luna Universal qPCR Master Mix (NEB, M3003) was used for qPCR employing 2 μL of cDNA sample and 18 μL mix of master mix (water ...
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bioRxiv - Molecular Biology 2022Quote: ... Luna Universal qPCR master mix (New England Biolabs) was used with 600 pM primer mix and 5 ng DNA per reaction ...
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bioRxiv - Cell Biology 2022Quote: ... the Luna Universal qPCR Master Mix protocol (NEB) was followed.
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bioRxiv - Cell Biology 2021Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used are listed in Suppl ...
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bioRxiv - Developmental Biology 2021Quote: ... and quantified using qPCR (Kapa Biosystems or NEB). Libraries were pooled for multiplex sequencing ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed using Luna Universal Mastermix (NEB), denaturation/annealing/elongation was performed for 50 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). For splicing verification ...
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bioRxiv - Cell Biology 2024Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used can be found in Suppl ...
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bioRxiv - Cancer Biology 2022Quote: ... Luna Universal qPCR MasterMix (NEB, cat. no. M3003) was used according to manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... using the Luna qPCR Mastermix (New England Biolabs) and normalized to Gapdh housekeeping gene ...
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bioRxiv - Cell Biology 2024Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used can be found in Suppl ...
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bioRxiv - Genomics 2024Quote: ... with Luna Universal qPCR Master Mix (NEB M3003E). Relative transcript abundance was normalized to GAPDH and non-targeting control gRNAs (ΔΔCt method) ...
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bioRxiv - Cell Biology 2024Quote: ... using the Luna Universal qPCR Master Mix (NEB). Primers for qPCR are listed in the Key resource table.
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bioRxiv - Microbiology 2024Quote: ... Luna Universal One-Step RT-qPCR Kit (NEB) was used to prepare cDNA and as reagent for RT-qPCR according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Luna® Universal Probe qPCR Master Mix (NEB) with 250nM of primers and probes was used in all reactions ...
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bioRxiv - Immunology 2024Quote: ... The 16S rRNA gene spanning variable regions V3+V4 was amplified using the broad-range forward primer 341F: CCTAYGGGRBGCASCAG and the reverse primer 806R: GGACTACNNGGGTATCTAAT using the Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program consisted of (1 ...
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bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Microbiology 2021Quote: ... A K13 propeller domain-specific guide gRNA was introduced into this vector at the BbsI restriction sites using the oligo pair p1+p2 (Table S9) using T4 DNA ligase (New England BioLabs). Oligos were phosphorylated and annealed prior to cloning ...
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bioRxiv - Synthetic Biology 2020Quote: ... inverted PgolB promoter with Bxb1 recognition sites and reporter-terminator (GFP-rrnBT1) pair were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (M0491, NEB) in thermal cycler (C1000 Touch ...
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bioRxiv - Physiology 2021Quote: ... A K13 propeller domain-specific guide gRNA was introduced into this vector at the BbsI restriction sites using the oligo pair p1+p2 (Supplementary file 5) using T4 DNA ligase (New England BioLabs). Oligos were phosphorylated and annealed prior to cloning ...
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bioRxiv - Microbiology 2022Quote: ... The first primer pair includes a multiple cloning site and both fragments were assembled using NEBuilder Hifi Assembly Cloning kit (New England Biolabs) together with the promoter and 5’ UTR of the constitutive expressed flavodoxin C4N14_09865 amplified from genomic DNA of F ...
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bioRxiv - Bioengineering 2023Quote: ... The 2 pairs of ends were then ligated simultaneously to the linearized plasmid using T4 DNA Ligase (New England Biolabs:M0202) at 2 U/pmol DNA ends in 1x T4 ligase buffer (provided with enzyme) ...
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bioRxiv - Cell Biology 2023Quote: ... and 21-base pair (bp) barcodes (post-culture) were amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Primers used are listed in Supplemental Table 11 ...
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bioRxiv - Microbiology 2023Quote: ... 600 - 700 base pair flanking regions of the coding region were amplified using Q5 high fidelity polymerase (New England Biolabs). Recombinant DNA used in this study is listed in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding oligo pairs were hybridized and inserted into the pLentiGuide vectors by GoldenGate cloning using BsmBI-V2 and T4 Ligase (NEB) then transformed in NEB stable cells ...
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bioRxiv - Genomics 2024Quote: ... 40 fmol of plasmid backbone and 2 μL of each annealed oligo pair were added to a one-pot golden gate reaction with Esp3I and T4 DNA Ligase (NEB) as has been done in previous studies73,75 ...
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bioRxiv - Developmental Biology 2024Quote: ... a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S. pyogenes Cas9 NLS from NEB, M0646T) were injected into the C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 2nd generation (eggs) was determined by qPCR following the Luna® Universal qPCR Master Mix Protocol (M3003, New England Biolabs) using 2 μl of DNA template ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA preparations were then used directly for qPCR (200 ng total RNA per reaction) using the Luna Universal One-Step RT-qPCR Kit (NEB, E3006) and target-specific primer/probe sets (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... from COLO205 cells was subjected to one-step RT-qPCR analysis using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...