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Citations for New England Biolabs :
751 - 800 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... primers AngiCOI_forward (S0963) and AC1R (S0966) were used in a qPCR reaction with Luna® Universal qPCR Mastermix (New England Biolabs, Australia). The qPCR reactions were run on the CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5ul of the resulting cDNA was used as template in 20ul scale qPCR reactions using Luna Universal qPCR master mix (New England Biolabs M3003) with forward primers specific to the microRNA of interest and a universal reverse primer ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the Luna universal qPCR master mix (NEB, M3003X). Gene expression levels were normalized to housekeeping genes EF1 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Genomics 2022Quote: ... mScarlet and HPRT (housekeeping gene for normalization) levels were then measured by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005) with 100ng total RNA per reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Titration of viral particles was performed by quantitative PCR (qPCR) using serial dilutions of purified AAV concentrates and Luna Universal qPCR mix (NEB#M3003). Oligonucleotide primers for AAV titration purpose were forward woodchuck hepatitis virus post-transcriptional regulatory element (WPRE ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Systems Biology 2024Quote: ... 10 ng nuclear RNA and 100 ng whole-cell RNA was used as template for RT-qPCR using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005). PCR was performed per manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... and RPL5 mRNA knockdown efficiency were assayed by real-time quantitative reverse-transcription PCR (RT-qPCR) using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were diluted at a 1:5 ratio with H2O prior to qPCR using Luna Universal qPCR Master Mix (New England Biolabs, #M3003) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... a custom 3’ adapter (5’-rAppCTGTAGGCACCATCAAT–NH2-3’, NEB, S1315S) was ligated to all RNAs ...
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bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta, New England BioLabs, cat. No. C3020K) and plated onto Carbenicillin plates ...
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bioRxiv - Systems Biology 2023Quote: ... The concentrated ligated products were then transformed into NEB 10-beta Electrocompetent E.coli (New England Biolabs, Ipswitch, MA). Three transformations were performed to increase the yield ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess RT primers were removed with exonuclease I (M0293S, NEB), and the cDNAs were size-selected (120-220 nt ...
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bioRxiv - Biochemistry 2020Quote: ... The reformatting primers introduce XhoI and XbaI (New England Biolabs) restriction endonuclease sites which allow for in-frame cloning of the VHH sequence with the α-factor secretion signal and includes a vector independent 6xHis tag at the C-terminus ...
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bioRxiv - Microbiology 2020Quote: ... Purified RNAs were random primed using Random Primer 9 (NEB) by incubation at 65°C for 5 minutes followed by rapid cooling on ice ...
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bioRxiv - Genomics 2022Quote: ... 900 nM primers and 250 unit/mL Eael (NEB, R0508S) in 1X ddPCR Supermix for Probes (no dUTP ...
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bioRxiv - Microbiology 2020Quote: ... Primers (Integrated DNA Technologies) were designed with NEBuilder (www.nebuilder.com, NEB) to incorporate the appropriate 5’ ends necessary for Gibson Assembly and 3’ gene specific ends ...
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bioRxiv - Molecular Biology 2020Quote: ... and reverse transcribed using random primer mix (New England Biolabs) and the Maxima Reverse Transcriptase kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... using Dual Index Primers (NEBNext Multiplex Oligos for Illumina, NEB). Libraries were sequenced on the NextSeq 500 platform (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... NEBNext Multiplex Oligos for Illumina Index Primers (New England Biolabs) were used for library preparation ...
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bioRxiv - Synthetic Biology 2022Quote: ... oligos were amplified with primers via Q5 DNA polymerase (NEB) using 0.2 picomoles of DNA as a template for 7 cycles ...
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bioRxiv - Microbiology 2022Quote: ... The unused primers were then degraded with Exonuclease I (NEB). cDNA from each set of 384 wells (one pool per plate ...
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bioRxiv - Immunology 2022Quote: ... Excess primers were digested by exonuclease ExoI (New England Biolabs). Beads were counted and the reverse transcribed cDNA libraries were amplified in a PCR reaction ...
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bioRxiv - Plant Biology 2023Quote: ... and 2μl random primer mix (New England Biolabs, cat#S1330S) were mixed and incubated for 10 minutes at 23°C then put on ice for 1 minute ...
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bioRxiv - Bioengineering 2024Quote: ... The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... and primers generated by NEBaseChanger (New England Biolabs, Ipswich, MA). The entire plasmid was sequenced (Plasmidsaurus ...
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bioRxiv - Synthetic Biology 2023Quote: Primers were generated using the NEBuilder software (New England Biolabs) using the following parameters ...
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bioRxiv - Cell Biology 2023Quote: ... and the primers are phosphorylated using polynucleotide kinase prior (NEB) to PCR such that each PCR product strand can be immediately ligated to itself ...
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bioRxiv - Genomics 2024Quote: ... Excessive primers were digested by Thermolabile Exonuclease I (NEB, M0568) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Index primers (NEBNext Multiplex Oligos for Illumina, New England Biolabs) were incorporated by PCR and indexed fragments of interest were adsorbed using 1.2 volumes of AMPure XP beads ...
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bioRxiv - Developmental Biology 2024Quote: ... primers were removed with an exonuclease (ExoI NEB, Cat#: M0293L), and the barcoded cDNAs for each single cell in a half-plate were pooled together for the next steps ...
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bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003, New England Biolabs, MA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... Transcript knockdown was confirmed in T0 and T1 plants using qPCR (qScript cDNA synthesis kit, QuantaBio®, and Luna qPCR mix; NEB®) according to manufactuers’ protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Four µl cDNA was used as input for 10 µl qPCR reactions with 2x Luna Universal qPCR Master Mix (NEB cat. #M3003X) and 0.25 µM forward and reverse primers (Extended Data Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... immitis and Wolbachia qPCR assays were carried out in 10 μL final volumes of Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia) containing 1 μL of template DNA isolated from whole dog blood and primers and probe at a final concentration of 400 nM and 100 nM ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA samples were quantified and used as templates for one-step RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB, MA, USA). Real time PCR amplification was performed using CFX96 touch real-time PCR detection system (Biorad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using Luna Universal qPCR master mix (NEB, Ipswich, MA). Primer sequences can be found in Table S17 ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... or Luna Universal One-Step RT-qPCR Kit (NEB) and 1.5 μl of reference primer/probe sets CDC-N1 (IDT 10006713 ...
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bioRxiv - Immunology 2022Quote: ... and the Luna Universal qPCR Master Mix (NEB, M3003). Final quantification was performed using the ΔΔCT method.
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using Luna 2x Master Mix (NEB) on an iQ5 thermocycler (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR reactions were assembled with Luna Master Mix (NEB) and qPCR was performed using a HT7900 (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... including Luna® Universal qPCR Master Mix (NEB, UK), one primer pair (cDNA template ...
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bioRxiv - Microbiology 2022Quote: The resultant libraries were quantified using qPCR (NEB E7630S) and sequenced on a Miseq (Illumina ...