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Citations for New England Biolabs :
951 - 1000 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Primer sets were used with Fusion high fidelity DNA polymerase (NEB M0530L) with the manufacturer-recommended protocol and standard molecular cloning techniques (Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... The NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, USA) was used for multiplexing ...
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bioRxiv - Molecular Biology 2023Quote: ... dinucleotide primers were 5’-labeled using T4 polynucleotide kinase (New England Biolabs) and added at 20 µM without pre-annealing to the template ...
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bioRxiv - Bioengineering 2024Quote: ... Primers for SDM (see Supplementary Table S3) were designed using NEBaseChanger (NEB) and synthesized as standard DNA oligos (IDT) ...
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bioRxiv - Biochemistry 2024Quote: ... which was performed with the NEBNext Universal PCR Primer for Illumina (NEB) and indexed primers (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Neuroscience 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB). All libraries were sequenced 150bp paired end using an Illumina Novaseq 6000 with 15 million reads on average per sample.
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bioRxiv - Cell Biology 2024Quote: ... using primers flanked by ClaI and XbaI restriction enzymes (New England Biolabs), digested and inserted into pcDNA3-TTP-mycHis vector backbone digested with the same restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL 200 ng/µL random nonamer (Random Primer 9, NEB). This mixture was heat denatured at 98° C for 1 minute ...
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bioRxiv - Microbiology 2024Quote: ... Colonies were screened using pET21_F and pET21_R primers and Q5 Polymerase (NEB) (See Table S4 for primer details and PCR conditions) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µM i7 index primer and nuclease-free water (New England Biolabs) up to a total volume of 50 µL ...
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bioRxiv - Systems Biology 2024Quote: ... 0.2 uM of reverse primers (L401) and OneTaq® DNA polymerase (NEB). DNA bands were visualized on 2% agarose gel containing ethidium bromide ...
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bioRxiv - Developmental Biology 2024Quote: ... Primers were used in RT-PCR reactions using OneTaq master mix (NEB) and PCR products were electrophoresed on a 1% agarose gel ...
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bioRxiv - Biochemistry 2024Quote: ... followed by cDNA synthesis with oligoT primers (ProtoScript II, New England Biolabs). The gene fragments lacking the part coding a transit peptide and pET15b plasmid (Novagen ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Microbiology 2021Quote: ... coli NEB 10-beta via the heat shock method of transformation according to the manufacturer’s instructions (New England Biolabs, Tokyo, Japan). All plasmids were extracted using the QIAprep Spin miniprep kit (QIAGEN ...
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bioRxiv - Molecular Biology 2023Quote: ... The tube containing the plugs was then cooled to 42°C before adding 5 µl of Beta-Agarase I (New England Biolabs, M0392) and incubating at 42°C for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR amplified products and cloning vector (pcDNA3.1) were digested to create compatible sites for ligation and transformed into NEB10 beta competent bacteria (NEB, Cat# C3019H). To create plasmids for chick electroporation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was then returned to ice for 5 minutes before 180 μL of NEB® 10-beta/Stable Outgrowth Medium (B9035, NEB) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR experiments were performed using SsoAdvanced Universal SYBR Green Supermix (NEB) on the Quantstudio5 (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used ...
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bioRxiv - Genomics 2022Quote: ... and Luna Universal qPCR Master Mix at 1X concentration (NEB, M3003L) in total reaction volume of 20 µL ...
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bioRxiv - Genetics 2022Quote: ... we used the Luna One Step RT-qPCR kit from NEB according to the manufacturer’s instructions at 10μl total volume in 384 well plates ...
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bioRxiv - Microbiology 2022Quote: ... Luna® Universal qPCR Master mix (New England Biolabs, cat# M3003L) was employed for RT-qPCR according to manufacturer’s recommendations (Initial denaturation 95°C ...
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bioRxiv - Microbiology 2021Quote: ... using Luna® Universal qPCR Master Mix (New England Biolabs, USA) and gene-specific primers (Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... qRT PCR was performed using Luna Universal qPCR master mix (NEB) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 5 µl of Luna® universal qPCR master mix (#M3003L, NEB), and 3 µl of nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... or Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Primer sequences used are described in Table S2 ...
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bioRxiv - Microbiology 2022Quote: qPCR was performed on cDNA using Luna (New England BioLabs #M3003) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... by Luna Universal One-Step RT-qPCR Kit (New England BioLabs) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were amplified with Luna Universal qPCR Master Mix (NEB, USA) according to manufacturer’s instructions in a Mx3005 qPCR system (Agilent ...
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bioRxiv - Physiology 2024Quote: ... and Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA). Targeted genes related to gluconeogenesis and glucose transporter were ...
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bioRxiv - Microbiology 2024Quote: ... using the Luna universal qPCR Master Mix (New England Biolabs, M3003) for cellular genes or the Luna universal probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... run and qPCR with NEBNext Library Quant kit (New England Biolabs) respectively ...
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bioRxiv - Microbiology 2023Quote: ... which contained 10 μL Luna Universal qPCR mastermix (New England Biolabs Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression was determined using Luna Universal qPCR Master Mix (BioLabs) using Roche LightCycler96 system ...
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bioRxiv - Genetics 2023Quote: ... 5μL of 2× Luna Universal qPCR Master Mix (NEB, MA, USA). The amplification program was set as follows ...
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bioRxiv - Systems Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB, #E3005). No template and genomic DNA controls were included in all experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Luna® Universal One-Step RT-qPCR Kit (NEB, Ipswich, Massachusetts) was used to quantify the RNA samples per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of viral genomes in the heat-inactivated samples performed through reverse transcription quantitative polymerase chain reaction (RT-qPCR) ...
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bioRxiv - Bioengineering 2023Quote: ... and according to the Luna Universal qPCR Master Mix protocol (NEB). Reaction volumes of 10 μL ...
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bioRxiv - Cell Biology 2023Quote: ... using a universal qPCR master mix (New England Biolabs, Ipswich, MA). All qPCR primer sequences are in Table S2 ...
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bioRxiv - Neuroscience 2024Quote: ... and Luna® Universal qPCR Master Mix (New England Biolabs M3003), according to the manufacturer’s Protocol for Two-step RT-qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Microbiology 2024Quote: ... the Luna Universal qPCR Master Mix Protocol (M3003 New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR was performed using LUNA SYBR green reagent (New England Biolabs) on a Qiagen Rotorgene instrument ...
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bioRxiv - Genetics 2024Quote: ... using Luna® Universal One-Step RT-qPCR Kit (NEB, Cat.M3005) and a Bio-Rad 96-well qPCR machine (CFX96 Touch Real-Time PCR Detection System) ...
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bioRxiv - Microbiology 2024Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of genomic SARS-CoV-2 RNA through RT-qPCR using a thermocycler (QuantStudio 6 thermocycler ...
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bioRxiv - Molecular Biology 2024Quote: ... with a Luna Universal qPCR Master Mix Kit (New England Biolabs). The target mRNA expression levels were normalized to those of the actin gene ...