Labshake search
Citations for New England Biolabs :
7651 - 7700 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The RNF114 C8A mutant was generated with Q5 site-directed mutagenesis kit according to manufacturer’s instructions (New England Biolabs, E0554S). Expression and purification conditions were optimized as reported previously79 ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into paired-end libraries using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Approximately 60 libraries were pooled per lane prior to sequencing on a HiSeq 4000 machine to a target depth of 0.5×.
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bioRxiv - Systems Biology 2019Quote: ... RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). Sequencing was performed on an Illumina NextSeq 500 (75 cycles) ...
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bioRxiv - Molecular Biology 2019Quote: ... and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit, New England BioLabs). The resulting RNA was purified using Silane beads (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Insert and dual indexed libraries were prepared using a modified NEBNext Ultra DNA II dual indexing kit (New England Biolabs) without size selection ...
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bioRxiv - Microbiology 2019Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis of low copy number vector p3187 harbouring siiA::HA under control of PsiiA was performed by using the Q5 SDM kit according to the manufacturer instructions (NEB) (Table S9) ...
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bioRxiv - Cell Biology 2019Quote: ... The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitated and input material was phenol-chloroform purified and ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library kit (NEB) according to the manufacturer’s instructions with 35ng of DNA of each sample ...
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bioRxiv - Bioengineering 2019Quote: ... The pgRNAMPN142-400 plasmid is constructed by removing the gRNAMPN372 from the pgRNA-triple-target plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Bioengineering 2019Quote: ... and 21 µg of gRNA transcripts produced by in vitro transcription of oligonucleotides (HiScribeTM T7 High Yield RNA Synthesis kit from NEB). Plugs are then treated overnight at 50°C with 10 µL of proteinase K ...
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bioRxiv - Microbiology 2019Quote: ... DNA-depleted RNA was processed for Illumina sequencing using the NEB Next Ultra Directional RNA Library Prep kit for Illumina as described by the manufacturer (NEB). Sequencing was performed using Illumina HiSeq ...
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bioRxiv - Molecular Biology 2019Quote: ... Then these two fragments were assembled into the modified pIRES vector at AsisI and PmeI sites using Gibson Assembly kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries were prepared using NEB’s NEBNext Ultra II Directional RNA Library Prep Kit for Illumina Sequencing (NEB EE7760S) and following the instructions for the poly(A ...
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bioRxiv - Biochemistry 2019Quote: ... were generated as described previously.23 In vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications ...
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bioRxiv - Microbiology 2019Quote: ... Library quality was assessed using an Agilent Tapestation 4200 instrument and quantity determined by qPCR using an NEBnext library quantification kit (NEB).
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bioRxiv - Biochemistry 2021Quote: cRNA were synthesized in vitro from linearized DNA of EGFP-fused mTTYH constructs using HiScribe T7 ARCA mRNA Kit (New England Biolabs). 1–2 ng of cRNA were injected in Xenopus laevis oocytes and imaged after 18 hours of incubation at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... primers oJV5-6 were used to remove ackA from pJV204 using the Q5 site-directed mutagenesis kit (NEB CN# E0554S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting cell pellets were washed in 1x PBS and resuspended in 1x DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Subsequently ...
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bioRxiv - Genetics 2020Quote: ... CUT&RUN dsDNA samples were quantified with a Qubit III and 5-10ng used as input for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645S, NEB). Libraries were prepared using the following PCR program ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
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bioRxiv - Genomics 2021Quote: ... Shotgun sequencing was performed using a PCR-free DNA library preparation (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs). Libraries were paired end 150 bp sequenced with Illumina HiSeqX ...
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bioRxiv - Genetics 2019Quote: ... and non-directional high-throughput sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). Libraries were processed on the Illumina HiSeq platform for single-end 50-nt sequencing ...
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bioRxiv - Genetics 2019Quote: ... and used to construct DNA sequencing libraries using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs E7760). DNA libraries were processed on a Illumina NextSeq machine for paired-end 42-nt sequencing ...
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with SPRI beads (0.9X) and quantified on a bioanalyzer and with NEB Illumina quant kit (NEB#E7630). HiC libraries were sequenced on a NextSeq500 either 150bp or 75bp paired-end reads.
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
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bioRxiv - Genomics 2021Quote: The RNA-seq libraries were prepared as follows: the rRNA was depleted from 0.5 µg of A549 total RNA using the NEBNext rRNA Depletion Kit (NEB E6310). The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and prepared DNA-Seq libraries from 100 ng DNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs; 12 minutes enzymatic shearing ...
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bioRxiv - Genomics 2021Quote: ... or the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (both New England Biolabs).
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized using 200 ng total RNA with the ProtoScript® First Strand cDNA Synthesis kit (New England Biolabs) utilizing random hexamers ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted from adult prothoracic leg tissue tissue using Monarch Total RNA Mini Kit (New England Biolabs, Ipswich, MA). Paired-end RNA-Seq libraries were constructed with NEBNext Ultra II Directional RNA prep (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated using the SuperScript IV reverse transcriptase kit (TFS) and PCR carried out using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... β-ENaC/SCNN1B (Hs00165722_m1) and γ-ENaC/SCNN1G (Hs00168918_m1) were performed with the Luna Universal Probe One-Step RT-qPCR Kit (NEB, E3006L). Expression of each gene was normalized to 18S (Hs99999901_s1) ...
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bioRxiv - Plant Biology 2020Quote: T-DNAs expressing four sgRNAs were assembled from BsaI-linearized vectors and three PCR fragments using an NEBuilder kit (E2621, New England Biolabs). Appropriate PCR fragments were generated in three- or four-primer reactions similar to the ones described above ...
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bioRxiv - Plant Biology 2020Quote: ... PolyA enrichment and library preparation were performed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Libraries were quantified by fluorometry ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting fragments were gel-purified with the QIAquick Gel Extraction Kit and ligated using T4 DNA Ligase (New England Biolabs) into dephosphorylated pBlueScript KS+ that had also been digested with NotI and gel-purified to make pBS-SS-TOM ...
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bioRxiv - Microbiology 2021Quote: ... or 230 nucleotides (rLLOV-ZsG-IRins-MARVUTR+tr) were excised and purified via Monarch DNA Gel Extraction Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Sequencing was performed at the Cornell University Transcriptional Regulation and Expression Facility on a NextSeq500 instrument (Illumina ...
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bioRxiv - Physiology 2021Quote: ... Three mRNA-seq libraries per condition were prepared using the NEBNext Ultra II directional RNA library Prep kit for Illumina (New England Biolabs), and read in paired-end 40-cycle sequencing runs on the NextSeq 500 system (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Genetic parts were amplified using primers listed in Table S1 and PCR products were purified with the Monarch PCR & DNA Clean up kit (NEB). Parts were assembled into plasmid constructs mainly by Gibson assembly [41] using the isothermal method ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Microbiology 2020Quote: ... metagenomic libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, Cat. No. E7645) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Sequencing libraries were generated by the Genome Analysis and Technology Core at University of Virginia using oligo dT-purified mRNA from 500ng of total RNA and the NEB Next Ultra RNA library preparation kit (New England Biolabs), and 50-bp single-end sequencing was performed on Illumina NextSeq 500 platform (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... The final amplicon was purified from genomic DNA using a Monarch PCR and DNA Cleanup Kit (New England Biolabs T1030) and quantified with a Qubit 2.0 Fluorometer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A library with 300-400 bp insert size for each pool was prepared using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). Libraries were then sequenced with 150 bp paired-end on the HiSeq X Ten platform.
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bioRxiv - Cancer Biology 2021Quote: ... mutant at A573D of AR-fl and AR-V7 in the pEGFP-C1 backbone were generated by site-directed mutagenesis using the Q5 site-Directed Mutagenesis Kit (New England BioLabs). The dimerization box (D-box ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Dual-indexed strand-specific RNA-seq library were prepared from the submitted total RNA samples using RiboZero rRNA depletion and the NEBNext Ultra Directional RNA library preparation kits (New England Biolabs). Paired-end 2×150bp sequencing was performed on an Illumina HiSeq 4000 platform ...
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bioRxiv - Biochemistry 2021Quote: ... which we used to construct the D614G mutant via site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs).
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bioRxiv - Biochemistry 2020Quote: ... The mRNA was then adapter ligated and prepared for Illumina sequencing using the Ultra Directional RNA Library Prep Kit (NEB).