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Citations for New England Biolabs :
6351 - 6400 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... E-Syt3ΔC2C was produced by introducing a 2021_2257 deletion using the Q5 site-directed mutagenesis kit (NEN Biolabs). The deletion created a reading frameshift and a premature stop that removed the C2C domain and created a 707 aa long protein with a 35 aa C-neoextension ...
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bioRxiv - Synthetic Biology 2021Quote: ... In vitro transcription was performed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs). The following reagents were combined ...
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bioRxiv - Pathology 2020Quote: ... linearized and transcribed in vitro using the Hi-Scribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA). GAPDH IVT-RNA was purified by precipitation with lithium chloride followed by column purification using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Zoology 2021Quote: NGS libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The resulting PCR products were purified and concentrated using AMPure XP beads (Beckman-Coulter) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA reaction mix was therefore compensated by adding 0.5 μl 5’Ligation Reaction Buffer 10x (NEB-kit) and 1.75 μl nuclease-free H2O.
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bioRxiv - Molecular Biology 2020Quote: ... Gel extraction was done using Gel breaker tubes (IST Engineering Inc) in DNA Gel Elution Buffer (NEB-kit). Disintegrated gels were incubated at 37°C for 1h on a shaker ...
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bioRxiv - Molecular Biology 2020Quote: Strand-specific RNA-seq libraries were constructed using the NEB Ultra II RNA-seq library kit (NEB #E7765) according to manufacturer’s protocol with fragmentation in first-strand buffer at 94°C for 15 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were prepared using Illumina NEBNext® UltraTM Directional RNA Library Prep Kit (NEB, cat. no. 7420) according to manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2019Quote: ... in vitro transcription was performed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). The mixture contained 10 µL of NTP buffer mix ...
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bioRxiv - Plant Biology 2020Quote: Library preparation was done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). To prepare samples ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB, #E7645) according to the manufacturer’s protocol followed by PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEB Next Ultra DNA Library Prep Kit for Illumina (New England Biolabs, USA) and sequenced on an Illumina Hiseq X-ten platform (150 bp paired-end reads) ...
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bioRxiv - Physiology 2021Quote: ... Sequencing libraries were generated with NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). RNA molecules were selected using poly-T oligo attached magnetic beads ...
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bioRxiv - Physiology 2021Quote: ... Library preparation was completed on purified mRNA using the NEBNext Ultra Directional RNA Library prep kit (NEB, #E7420L) using 14 cycles of PCR amplification and indexed using single (i7 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The obtained DNA fragments were subjected to library construction using the NEBNext™ DNA Library Prep Kit (NEB), following the supplier’s specifications ...
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bioRxiv - Developmental Biology 2020Quote: UASp-KibS677A-GFP-FLAG was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Catalog #E0554S) using primers KibS677A (see primers table) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (
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bioRxiv - Microbiology 2021Quote: ... and linker region were inserted into the relevant constructs also by site-directed mutagenesis (NEB Q5-based kit). Optimized DNA sequence is provided in figshare File 1 (10.6084/m9.figshare.c.5390285) ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using the NEBnext Ultra-directional RNA Library Kit for Illumina (New England Biolabs, Ipswitch, MA, USA) with NEBnext Multiplex Oligos for Illumina (Primer Set 1 ...
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bioRxiv - Microbiology 2021Quote: ... The DNA concentration was determined with the NEBNext Library Quant Kit for Illumina (New England Biolabs, MA, USA) and pooled in equimolar ratios with unique barcodes for each sample ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was purified from cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs, USA). Purified DNA samples were eluted in 30µl nuclease-free water and quantified on a Nanodrop One (Thermo Fischer Scientific ...
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bioRxiv - Zoology 2020Quote: ... Sequencing libraries were generated using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2020Quote: ... Fragments for assembly were purified using agarose electrophoresis and gel extraction (Monarch DNA Gel Extraction Kit, NEB T1020L). For assembly ...
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bioRxiv - Biochemistry 2021Quote: ... Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB), and the resulting library was sequenced using Illumina MiSeq v2 (2×150 bp ...
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bioRxiv - Cancer Biology 2019Quote: ... In vitro transcription was carried out by the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs). Resulting transcriptions products were treated with DNAse I ...
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bioRxiv - Microbiology 2021Quote: ... sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S).
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bioRxiv - Bioengineering 2020Quote: ... The RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s instructions [51] ...
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bioRxiv - Developmental Biology 2020Quote: ... Stranded cDNA libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primer Set I ...
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bioRxiv - Immunology 2020Quote: ... Illumina adaptors were added using the NEBNext® Ultra™ II DNA Library Prep Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis was performed using the NEB Q5 site-directed mutagenesis kit (NEB, Ipswitch, MA, cat #E0554S) using mutagenesis primers summarized in Table S2 with pBADmobshyA as a template.
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bioRxiv - Zoology 2019Quote: ... DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA), and NEBNext Multiplex Oligos for Illumina Index kit (New England BioLabs ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Libraries were sequenced using SE 75 bp Illumina NextSeq.
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bioRxiv - Developmental Biology 2019Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... digested pCFD6 backbone using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Cat. No: R5520S), following the instructions provided by the manufacturers.
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bioRxiv - Genetics 2020Quote: ... pyogenes chimeric single guide RNA through in vitro transcription using a HiScribe T7 Quick kit (New England Biolabs). The gRNA was then precipitated in a 1/10 volume of 3M Sodium Acetate and two volumes of 100% ethanol by chilling the reaction at −20 °C for 15 min ...
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bioRxiv - Genetics 2020Quote: ... The small RNA fraction was used with the NEBNext small RNA library prep kit (New England Biolabs, #E7300) to produce a small RNA library ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... We then created a construct containing the alternate allele using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were:
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bioRxiv - Genetics 2020Quote: ... The purified dephosphorylated linear plasmid and phosphorylated annealed oligos were ligated together using the Quick Ligation Kit (NEB). The ligated product was transformed into One Shot Stbl3 Chemically Competent E ...
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bioRxiv - Genetics 2020Quote: ... TrueSeq-barcoded RNA-seq libraries were generated with the NEBNext Small RNA Library Prep Kit (New England Biolabs) and size selected for insert sizes ∼18-50bp ...
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bioRxiv - Genetics 2020Quote: mRNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) as described (29) ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IgG /IgK/IgL NGS libraries were made by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on the Illumina Miseq 2 × 300 platform ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA libraries were generated according to NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760). cDNA was amplified for 12 cycles and barcoded with NEBNext Multiplex Oligos for Illumina (NEB E7600) ...
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bioRxiv - Genomics 2021Quote: The whole genome bisulfite (WGBS) libraries were prepared using the Ultra II DNA library prep kit (NEB E7645) before being Bisulfite converted using the Zymo EZ DNA Methylation-Gold bisulfite conversion kit according to the protocol described in Vaisvila et al ...
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bioRxiv - Immunology 2020Quote: ... and the quantity of the library was determined by qPCR using NEBNext Library Quant Kit for Illumina (NEB) according to manufacture’s instruction ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA libraries were made using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs # E7645S) according to the manufacturer’s recommendations.