Labshake search
Citations for New England Biolabs :
6501 - 6550 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). The extracted RNA was eluted in 50 μL of nuclease- free water and treated with Qiagen DNAse I (Germantown ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries for RNA-seq were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (E7760, NEB). The size of the libraries was determined using a Bioanalyzer ...
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bioRxiv - Genomics 2023Quote: ... Commercial and breeding program samples were extracted from frozen tissue using the Monarch Genomic DNA Purification Kit (NEB), as per manufacturer’ s instructions using the enzymatic cleanup protocol with both proteinase K and RNase A ...
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bioRxiv - Biochemistry 2024Quote: ... at 37°C for 30 min and purified by Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The concentration of crRNA was determined by Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and were sequenced on an Illumina NovaSeq 150bp paired-end platform ...
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bioRxiv - Genetics 2024Quote: ... RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II FS Library Prep Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s instructions with inserts of approximately 350 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, US-CA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was either extracted with phenol-chloroform followed by Monarch Total RNA Miniprep kit (New England Biolabs) or with the Quick-RNA Miniprep kit (Zymo) ...
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bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
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bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
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bioRxiv - Neuroscience 2024Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were extracted and assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The assembly product was transformed into E ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were completed with the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina” (NEB #E7805) and subsequently analysed on an Illumina instrument by following manufacturer’s protocols ...
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bioRxiv - Physiology 2024Quote: RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s polyA mRNA workflow at the Advanced Analysis Center at the University of Guelph (Guelph ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA (100 ng) was used for cDNA synthesis using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) with random hexamers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequenced on an Illumina HiSeq 3000 platform to generate a minimum of 20 million 150-bp reads per sample.
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Immunology 2024Quote: cDNAs were synthesized from isolated RNA samples using ProtoScript II First Strand cDNA Synthesis Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared from 1µg of RNA using the LunaScript® RT SuperMix Kit (New England Biolabs, E3010L). A no template control (NTC ...
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bioRxiv - Developmental Biology 2024Quote: ... the specific product was purified from the agarose gel using the Monarch DNA Gel Extraction Kit (NEB, T1020S). Each reverse primer contains the T3 RNA Polymerase minimal promoter sequence (shown in bold and underlined in Table 1) ...
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Immunology 2024Quote: ... IVT DI was in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs) followed by DNAse I clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... mApple was cloned in place of the eYFP gene using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and BstXI and NotI restriction sites as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microlitre reaction mixtures were prepared using the Luna® Universal qPCR Master Mix kit (New England Biolabs) as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Sequencing of the library was performed using Illumina HiSeq2500 and NextSeq2000 to produce 65-bp single-end reads and 36-bp paired-end reads ...
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bioRxiv - Genomics 2024Quote: Hadrurus arizonensis DNA was extracted using the MonarchⓇHigh Molecular Weight (HMW) DNA Extraction Kit: Tissue Protocol (NEB, T3060). Changes made to the protocol include ...
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bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
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bioRxiv - Biochemistry 2023Quote: ... The gRNA oligos were incorporated into gRNA-Cas9 vector using NEBuilder HiFi DNA Assembly kit (New England Biolabs). Each donor oligo contains a point mutation that replace original codon with alanine or stop codon flanked by 40 nt homologous recombination sequences on both 5’ and 3’ (all donor sequences and gRNA vectors are shown in supplementary files) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Genetics 2023Quote: ... Both point mutations were generated by site directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using the wild type part-containing plasmids as the template and the following primers ...
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bioRxiv - Genomics 2023Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB #E7645). End repair was carried out by resuspending beads in 50 μL 1 × EB buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Genomics 2023Quote: ... CBS 112042+ and CBS 124.78+ were sequenced on R9.4.1 flowcells using the LSK108 kit with 3 μg DNA as input for the end prep reaction (NEB ULTRA-II EP ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from dissected E14.5 DRG was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was synthesized with iScript cDNA synthesis kit (Biorad ...