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Citations for New England Biolabs :
6251 - 6300 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Japan) were inserted downstream of SaCas9 into the vector using a NEBuilder HiFi assembly kit (New England Biolabs). For construction of plasmid used for cell sorting ...
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bioRxiv - Microbiology 2021Quote: ... Treated RNA was used for cDNA library preparation by NEBNext Ultra II RNA library kit for Illumina (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Microbiology 2019Quote: ... Table S2) were created by site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England BioLabs). For -10 region mutations ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis or promoter deletions were performed with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). PCR reactions were done in 50 μL containing 25 μL of Q5 Hot Start High-Fidelity 2X Master Mix ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Constructs expressing GFP-tagged or HA-tagged Shank3 phospho-mutants were generated using the Gibson Assembly kit (NEB) with the wild-type Shank3 as the template ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA libraries were prepared by removing the ribosomal RNA with NEBNext rRNA depletion kit (New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB England BioLabs). Fragmented and randomly primed 2 × 150□bp paired-end libraries were sequenced using Illumina HiSeq X10.
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bioRxiv - Biophysics 2020Quote: ... Further truncation and point mutants of CreCat construct were prepared using the Q5 Site-Directed Mutagenesis Kit (NEB). Protein expression from E ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) with following changes to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Genomics 2021Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
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bioRxiv - Biochemistry 2022Quote: ChIP-seq libraries were prepared using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... Stranded cDNA libraries were generated using NEBNext Ultra Direction RNA Library Prep Kit for Illumina (New England Biolabs) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primer Set I ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... Bands were extracted for subsequent amplification as above and purification using a Monarch PCR Cleanup Kit (NEB T1030L). Purified products were sequenced commercially (ACGT ...
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bioRxiv - Neuroscience 2022Quote: ... The RNA-seq libraries were prepared using either the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and sequenced with the Novaseq 6000 platform (ILLUMINA ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly A selection was performed using NEB Next® Ultra ™ RNA Library Prep Kit (New England Biolabs) and the sequencing libraries (250∼300 bp insert cDNA library ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was subsequently extracted from T0 plants using Monarch DNA extraction kits (New England Biolabs, Ipswich, MA, USA) and sequence confirmed (CHU de Québec-Université Laval ...
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bioRxiv - Genomics 2022Quote: ... ChIP libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 ...
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bioRxiv - Genomics 2022Quote: ... Quality of the library was analyzed by Bioanalyzer and NEBNext® Library Quant Kit for Illumina® (NEB). IS libraries were sent for sequencing to c.ATG sequencing core facility at Tübingen University and sequenced on a MiSeq instrument 2×150 bp.
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bioRxiv - Microbiology 2022Quote: ... The online NEBasechanger (https://nebasechanger.neb.com/) was used to design primers and the Q5 Site Directed Mutagenesis Kit (NEB) was used to generate pUPRTKO-ISC6pro-AC9ΔCC-3xTy (primers P13-14) ...
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bioRxiv - Microbiology 2022Quote: ... Any host RNA and bacterial ribosomal RNA were depleted using the NEBNext rRNA Depletion Kits (New England BioLabs). Isolated RNA was then assessed for quality using the Agilent TapeStation system ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs; version 6.0 – 2/18) using NEBNext Multiplex Oligos for Illumina-Dual Index Primers Set 1 (#E7600S ...
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bioRxiv - Genetics 2022Quote: ... The kanamycin resistance cassette-gDNA junctions were prepared for sequencing using NEB Ultra I kit (New England Biolabs). Following adapter ligation and treatment with the NEB USER Enzyme ...
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bioRxiv - Bioengineering 2022Quote: mRNAs were transcribed from DNA templates using T7 RNA polymerase (HiScribe T7 High Yield RNA Synthesis Kit, NEB). To generate base modified mRNA variants ...
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Microbiology 2022Quote: ... Resultant fragments were then gel-purified using the Monarch DNA Gel extraction kit (New England Biolabs, MA, USA) and ligated with the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... UC Berkeley) (Chen et al 2017) were generated by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB), using specific primers containing the sgRNA 20-mer target sequence (split in half across the two primers) ...
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bioRxiv - Genomics 2022Quote: Libraries for sequencing were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material ...
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bioRxiv - Immunology 2022Quote: Plasmids containing templates for CRISPR-mediated KI were assembled using the NEBuilder HIFI assembly kit (New England Biolabs) starting from PCR amplified fragments ...
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bioRxiv - Microbiology 2022Quote: DNA libraries were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer’s protocol using ∼300 ng of DNA ...
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bioRxiv - Plant Biology 2022Quote: ... The K107E and K107T mutations were generated using the Q5 Site-Directed Mutagenesis Kit (E0552S, New England Biolabs) with primers listed in Supplemental Table 3 in pENTR/D-TOPO followed by transfer to pIX-HALO ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... the AlkY1355S HDR donor construct was modified accordingly using the Q5® site-directed mutagenesis kit (NEB, #E0554S). Due to the close proximity of both codons within the sequence ...
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cell Biology 2022Quote: ... then libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... the cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530S, New England Biolabs). Quality of the cDNA library was also assessed by Qubit assay for concentration ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Microbiology 2021Quote: ... The two amplicons were fused using a NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... as was the pCDH vector before purification again with Monarch® DNA Gel Extraction Kit (New England Biolabs). The ACE2 fragment was ligated with pCDH vector using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and cDNA was generated by reverse transcription using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB). cDNA was amplified with primers flanking each splice site by Taq polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA). PCR was performed with Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... library preparation was completed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). The adapters and PCR primers used were purchased from New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... strand-specific cDNA libraries were generated by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... We constructed libraries from 300 ng of RNA using NEB Next Ultra RNA kit (New England Biolabs, USA) and isolated Poly(A ...
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bioRxiv - Microbiology 2020Quote: ... Whole genome shotgun libraries were prepared using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA) for sequencing on either the MiSeq or HiSeq (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: Library preparation: TrAEL-seq adaptor 2 was added using a modified NEBNext Ultra II DNA kit (NEB E7645S): 3.5 μl NEBNext Ultra II End Prep buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... First strand cDNA was synthesised using a ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560) using oligo d(T)23 primers and 500 ng total RNA.