Labshake search
Citations for New England Biolabs :
6551 - 6600 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). The extracted RNA was eluted in 50 μL of nuclease- free water and treated with Qiagen DNAse I (Germantown ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: Ifnb1 mRNA 3’ UTR (NM_002176.4) was synthesized using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S) through in vitro transcription ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted from flash-frozen samples with the Monarch® Total RNA Miniprep Kit (New England Biolabs) including the DNase treatment step ...
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the Q5® Site-Directed Mutagenesis Kit from New England Biolabs (NEB; Ipswich, MA, USA), following their protocol with recommended annealing temperature and pETσ70 CFI ...
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bioRxiv - Microbiology 2023Quote: ... IVT-mRNAs were then purified using a Monarch RNA clean up kit (New England Biolabs, Ipswich, MA, USA), and the quality of purified IVT-mRNAs was assessed by non-denaturing agarose gel electrophoresis using RNA High for Easy Electrophoresis (DynaMarker Laboratory ...
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bioRxiv - Molecular Biology 2023Quote: Libraries for small RNA-seq were prepared using NEB Next® Multiplex Small RNA kit (New England Biolabs), and the libraries were sequenced by NovaSeq 6000 system (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... The RNA adaptor provided by the kit was phosphorylated on its 5’ end using T4 Polynucleotide Kinase (NEB). RNA (1 μg ...
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bioRxiv - Biochemistry 2023Quote: Site directed mutagenesis for I72W M.TaqI mutation on M.TaqI plasmid a was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...
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bioRxiv - Physiology 2023Quote: ... and mRNA was isolated and library prepped using the NEB Directional RNA sequencing kit (E7765L, New England Biolabs) with the PolyA purification bundle (E7490L ...
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bioRxiv - Microbiology 2023Quote: ... libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and sequenced on the Illumina NovaSeq 6000 Sequencer (2×150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-directed mutagenesis of DmelOrco was done by PCR with the Q5® site directed mutagenesis kit (NEB) using primers optimized with the NEBase Changer online tool and following the supplier’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Six variants were mutated on the basis of proPIF4-L:LUC using Q5 site-directed mutagenesis kit (NEB, E0552S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... amino acid change-conferring substitutions were placed into the SHIV.C.CH505.3C backbone using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs #E0554S). Primers were designed according to the NEBaseChanger tool (https://nebasechanger.neb.com) ...
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bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S) in conjunction with the NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... m6A-seq libraries were prepared using the NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626 ...
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bioRxiv - Molecular Biology 2023Quote: The SRRM1 entry clone from DNASU was modified using the Q5 site directed mutagenesis kit (New England Biolabs) to delete the predicted disordered region as annotated by Uniprot (https://www.uniprot.org/uniprotkb/Q8IYB3/entry) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA of wild-type and stat3 mutants were generated by the ProtoScript First Strand cDNA Synthesis Kit (NEB) using random primers ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and quality and concentration were assessed with the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... ONT Kit) was ligated to the RNA sample using 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2023Quote: RT-LAMP reactions were done using the WarmStart Colorimetric LAMP 2X Master Mix kit (M1800, NEB, Hitchin, UK) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Illumina libraries for RNAseq were prepared using NEBNext Ultra II RNA Library Prep kit for Illumina (E7770, NEB) with NEBNext Poly(A ...
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bioRxiv - Microbiology 2023Quote: ... library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) using normalized RNA inputs per sample and without direction RNA selection ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), including library amplification steps ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was completed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using MiSeq v3 Paired End 150 bp (Illumina).
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bioRxiv - Evolutionary Biology 2023Quote: ... and transformed cells with the resulting library after column-based purification with a commercial electroporation kit (NEB #T1020L). We grew populations of E ...
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bioRxiv - Microbiology 2023Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was amplified by performing a multiplexed PCR in two pools using the ARTIC-N6 primers and the Q5 High-Fidelity DNA polymerase or Q5 Hot Start DNA polymerase (New England BioLabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-genome libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) and sequenced on a NovoseqPE150 sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized on 2 µg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Strand-specific RNA-seq libraries were constructed using the NEB Ultra II RNA-seq library kit (NEB #E7765) according to manufacturer’s protocol with the following parameters ...
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bioRxiv - Genomics 2023Quote: ... WGBS single indexed libraries were generated using NEBNext Ultra DNA library Prep kit for Illumina (New England BioLabs) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: RNA libraries were prepared using the NEBNext Ultra II RNA Illumina Library Prep Kit (New England Biolabs, E7775) and mRNAs were enriched with oligo-d(T ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified after agarose gel electrophoresis using the Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for Sanger sequencing to Eurofins Genomics ...
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bioRxiv - Plant Biology 2023Quote: ... point or deletion mutants of pGH-dspE was obtained using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with following primer sets:
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bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
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bioRxiv - Biophysics 2023Quote: ... Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs), plasmids were transformed into E ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) together with the NEBNext Poly(A ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of the HA-AHR plasmid was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S).
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bioRxiv - Molecular Biology 2023Quote: ... and 25 mM EDTA followed by clean-up with a Monarch PCR & DNA Cleanup Kit (New England Biolabs). Second-strand DNA synthesis was done by ligating 10 µM of a blunt-end duplex comprised of a 32-bp Illumina R1-3’SpC3 and 5’-phosphorylated R1R-3’SpC3 DNA (pre-annealed by incubating at 95°C for 3 min and slowly cooling to 25°C ...
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bioRxiv - Molecular Biology 2023Quote: Ribosomal RNA was depleted from total RNA preparations with the NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs). Total RNA libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Amplified gene inserts were assembled with amplified pEVS143 by Gibson assembly using NEB Gibson Assembly Cloning Kit (NEB) or NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: The dsRNAs were transcribed from these templates using HiScribe T7 high yield RNA synthesis kit (New England Biolabs). The dsRNAs were then treated with DNase and purified using RNA Clean & Concentrator-25 (Zymo Research).