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Citations for New England Biolabs :
5051 - 5100 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs, Ipswich, MA). AAV-hSyn-hM3Dq-RAM-d2tTA was constructed as previously described19 ...
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bioRxiv - Cell Biology 2023Quote: ... 1ng pcDNA3.3-OCT4 was added for the amplification using the Q5 High-Fidelity DNA Polymerase kit (NEB, M0491). pcDNA3-EGFP (addgene ...
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bioRxiv - Genomics 2023Quote: Purified cDNA was used as an input for the Nextera XT DNA library preparation kit (New England Biolabs). Library quality and size was determined using a TapeStation (Agilent Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... A library was prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
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bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted from all samples with use of the NEBNext rRNA depletion kit (New England Biolabs). The samples were fragmented ...
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bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), using 1 μg total RNA per experiment.
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bioRxiv - Biochemistry 2023Quote: ... CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) following the manufacturer’s instruction with modifications to SPRI bead size selection and library amplification reactions to retain small DNA fragments(35) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qRT-PCR were performed simultaneously using the Luna Universal One-Step RT-qPCR kit (NEB) with the Quantstudio 7 Flex (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... The guide was cloned into the vector by Gibson Assembly (Gibson Assembly Cloning Kit; New England BioLabs #E5510S) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Benjamin Davies group) between the pair of BsaI site with the NEB Golden Gate Assembly Kit (NEB #E1601). The pROSA-TV2 was designed to contain the 1.4 and 1.2 homology arms of the inserted site and a hygromycin selection cassette that is flanked by a pair of lox sites (loxP) ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from 10 hpf embryos using a Monarch total RNA miniprep kit (New England BioLabs Inc.) RNA was stored at −80°C until sample analysis and sequencing by the Molecular Evolution Core at Georgia Tech as previously reported(Johnson et al ...
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bioRxiv - Microbiology 2023Quote: ... We generated sequencing libraries using the TruSeq DNA PCR-Free Sample Preparation Kit (New England Biolabs, Massachusetts, USA), which were sequenced using the Illumina MiSeq PE300 platform (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... The gRNA oligos were incorporated into gRNA-Cas9 vector using NEBuilder HiFi DNA Assembly kit (New England Biolabs). Each donor oligo contains a point mutation that replace original codon with alanine or stop codon flanked by 40 nt homologous recombination sequences on both 5’ and 3’ (all donor sequences and gRNA vectors are shown in supplementary files) ...
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bioRxiv - Molecular Biology 2023Quote: Pre-crRNA arrays (Supplementary Table 9) were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Microbiology 2023Quote: ... Hep3B cells and HBV control HepG2 2.2.15 cells and subjected to PCR amplification using the LongAmp Taq kit (NEB) and HBV primers spanning the CTCF binding motifs (HBV2 forward and Hep23B reverse) ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from roughly 500,000 HEK293T cells using the Monarch Genomic DNA Purification Kit (NEB T3010L) and fragmented by digestion with XbaI and AccI to excise and release the genomic DNA region corresponding to the AAVS1 reporter ...
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bioRxiv - Cell Biology 2023Quote: ... RNA extraction for other experiments was performed using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) according to the provided protocol.
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB #E7645). Note ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from flash frozen samples with the Monarch® Genomic DNA Purification Kit (New England Biolabs), including the on-column RNase treatment ...
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bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis and RT-qPCR was carried out using Luna® Universal One-Step RT-qPCR Kit (NEB) according to the manufacturer’s instructions with 100 ng of mRNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Micro-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) following77 manufacturer instructions and sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Molecular Biology 2023Quote: ... and used to construct Illumina sequencing libraries with NEBNext Ultra Library DNA Prep Kit for Illumina (NEB, E7370). BrdU-labeled nascent DNA library fragments were then enriched by immunoprecipitation with anti-BrdU antibody (BD ...
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bioRxiv - Genomics 2023Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB #E7645). End repair was carried out by resuspending beads in 50 μL 1 × EB buffer ...
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bioRxiv - Genomics 2023Quote: ... CBS 112042+ and CBS 124.78+ were sequenced on R9.4.1 flowcells using the LSK108 kit with 3 μg DNA as input for the end prep reaction (NEB ULTRA-II EP ...
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bioRxiv - Genetics 2023Quote: ... Both point mutations were generated by site directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using the wild type part-containing plasmids as the template and the following primers ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from dissected E14.5 DRG was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was synthesized with iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Genomics 2023Quote: ... The product was converted to double stranded DNA using the NEBNext second strand synthesis kit (New England Biolabs). Library preparation was carried out using a KAPA LTP kit (KAPA Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... Indexed Illumina sequencing libraries were constructed using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA).
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bioRxiv - Genomics 2023Quote: ... and further purified samples using a NEB Monarch® RNA Cleanup kit (New England Biolabs, Ipswich, MA, USA). We checked total RNA quantity and quality using a NanoDrop One spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... ZAP positive selection mutants were generated by the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) or synthesized as a gene block (Twist Bioscience ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing library preparation was made using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB) following the procedure described in “Library Prep for CUT&RUN with NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, Cat# E7530L), and were sequenced by Illumina NovaSeq 6000 sequencing machine with 150-bp paired-end reads by Beijing Novel Bioinformatics Co. ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was depleted from 0.8 μg total RNA using NEBNext rRNA Depletion Kit (New England Biolabs, E6350L) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... nosP-5’Eno-GFP-3’Eno and nosP-5’Eno-GFP-3’SV40 plasmids were generated by assembly of PCR fragments into the pNOSPE_MCP_eGFP vector 63 digested by NotI and BamHI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNOSPE_MCP_eGFP contains the nanos promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs). These samples were sequenced on the high-throughput sequencing platform (NovaSeq6000 ...
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bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was carried out using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs #E6560S) with random hexamer mix ...
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bioRxiv - Neuroscience 2023Quote: ... We prepared cDNA libraries from GFP+ and GFP-samples (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB) from RNA enriched with mRNA through bead-based polyA selection ...
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bioRxiv - Cell Biology 2023Quote: ... This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) to create a new HindIII site at the position encoding amino acid 569 ...