Labshake search
Citations for New England Biolabs :
4951 - 5000 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Immunology 2024Quote: ... IVT DI was in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs) followed by DNAse I clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... mApple was cloned in place of the eYFP gene using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and BstXI and NotI restriction sites as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microlitre reaction mixtures were prepared using the Luna® Universal qPCR Master Mix kit (New England Biolabs) as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Sequencing of the library was performed using Illumina HiSeq2500 and NextSeq2000 to produce 65-bp single-end reads and 36-bp paired-end reads ...
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bioRxiv - Genomics 2024Quote: Hadrurus arizonensis DNA was extracted using the MonarchⓇHigh Molecular Weight (HMW) DNA Extraction Kit: Tissue Protocol (NEB, T3060). Changes made to the protocol include ...
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bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
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bioRxiv - Biochemistry 2023Quote: ... The gRNA oligos were incorporated into gRNA-Cas9 vector using NEBuilder HiFi DNA Assembly kit (New England Biolabs). Each donor oligo contains a point mutation that replace original codon with alanine or stop codon flanked by 40 nt homologous recombination sequences on both 5’ and 3’ (all donor sequences and gRNA vectors are shown in supplementary files) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Genetics 2023Quote: ... Both point mutations were generated by site directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using the wild type part-containing plasmids as the template and the following primers ...
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bioRxiv - Genomics 2023Quote: Final libraries were prepared on beads using the NEB-Next Ultra II DNA Library Prep Kit (NEB #E7645). End repair was carried out by resuspending beads in 50 μL 1 × EB buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Genomics 2023Quote: ... CBS 112042+ and CBS 124.78+ were sequenced on R9.4.1 flowcells using the LSK108 kit with 3 μg DNA as input for the end prep reaction (NEB ULTRA-II EP ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from dissected E14.5 DRG was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was synthesized with iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... nosP-5’Eno-GFP-3’Eno and nosP-5’Eno-GFP-3’SV40 plasmids were generated by assembly of PCR fragments into the pNOSPE_MCP_eGFP vector 63 digested by NotI and BamHI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNOSPE_MCP_eGFP contains the nanos promoter ...
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bioRxiv - Genomics 2023Quote: ... Indexed Illumina sequencing libraries were constructed using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA).
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bioRxiv - Genomics 2023Quote: ... The product was converted to double stranded DNA using the NEBNext second strand synthesis kit (New England Biolabs). Library preparation was carried out using a KAPA LTP kit (KAPA Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... and further purified samples using a NEB Monarch® RNA Cleanup kit (New England Biolabs, Ipswich, MA, USA). We checked total RNA quantity and quality using a NanoDrop One spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 3.) Library preparation was performed using the NEBNext Ultra II library preparation kit for Illumina (New England Biolabs) and 4. ...
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bioRxiv - Plant Biology 2023Quote: ... Six variants were mutated on the basis of proPIF4-L:LUC using Q5 site-directed mutagenesis kit (NEB, E0552S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... amino acid change-conferring substitutions were placed into the SHIV.C.CH505.3C backbone using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs #E0554S). Primers were designed according to the NEBaseChanger tool (https://nebasechanger.neb.com) ...
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bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S) in conjunction with the NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... m6A-seq libraries were prepared using the NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626 ...
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bioRxiv - Molecular Biology 2023Quote: The SRRM1 entry clone from DNASU was modified using the Q5 site directed mutagenesis kit (New England Biolabs) to delete the predicted disordered region as annotated by Uniprot (https://www.uniprot.org/uniprotkb/Q8IYB3/entry) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA of wild-type and stat3 mutants were generated by the ProtoScript First Strand cDNA Synthesis Kit (NEB) using random primers ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and quality and concentration were assessed with the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... ONT Kit) was ligated to the RNA sample using 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the Q5® Site-Directed Mutagenesis Kit from New England Biolabs (NEB; Ipswich, MA, USA), following their protocol with recommended annealing temperature and pETσ70 CFI ...
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bioRxiv - Microbiology 2023Quote: ... IVT-mRNAs were then purified using a Monarch RNA clean up kit (New England Biolabs, Ipswich, MA, USA), and the quality of purified IVT-mRNAs was assessed by non-denaturing agarose gel electrophoresis using RNA High for Easy Electrophoresis (DynaMarker Laboratory ...
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bioRxiv - Molecular Biology 2023Quote: Libraries for small RNA-seq were prepared using NEB Next® Multiplex Small RNA kit (New England Biolabs), and the libraries were sequenced by NovaSeq 6000 system (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... The RNA adaptor provided by the kit was phosphorylated on its 5’ end using T4 Polynucleotide Kinase (NEB). RNA (1 μg ...
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bioRxiv - Biochemistry 2023Quote: Site directed mutagenesis for I72W M.TaqI mutation on M.TaqI plasmid a was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...
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bioRxiv - Physiology 2023Quote: ... and mRNA was isolated and library prepped using the NEB Directional RNA sequencing kit (E7765L, New England Biolabs) with the PolyA purification bundle (E7490L ...
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bioRxiv - Microbiology 2023Quote: ... libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and sequenced on the Illumina NovaSeq 6000 Sequencer (2×150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-directed mutagenesis of DmelOrco was done by PCR with the Q5® site directed mutagenesis kit (NEB) using primers optimized with the NEBase Changer online tool and following the supplier’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) to create a new HindIII site at the position encoding amino acid 569 ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted from all samples with use of the NEBNext rRNA depletion kit (New England Biolabs). The samples were fragmented ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from 10 hpf embryos using a Monarch total RNA miniprep kit (New England BioLabs Inc.) RNA was stored at −80°C until sample analysis and sequencing by the Molecular Evolution Core at Georgia Tech as previously reported(Johnson et al ...
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qRT-PCR were performed simultaneously using the Luna Universal One-Step RT-qPCR kit (NEB) with the Quantstudio 7 Flex (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) following the manufacturer’s instruction with modifications to SPRI bead size selection and library amplification reactions to retain small DNA fragments(35) ...
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bioRxiv - Cancer Biology 2023Quote: RNA libraries were prepared using the NEBNext Ultra II RNA Illumina Library Prep Kit (New England Biolabs, E7775) and mRNAs were enriched with oligo-d(T ...
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bioRxiv - Genomics 2023Quote: ... the products of PCR1 were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L) and deep sequenced at the MGH DNA Core ...