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Citations for New England Biolabs :
5201 - 5250 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Second end processing and library amplification were performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2021Quote: sgRNA products synthesized by T7 RNAP WT and S43Y mutant were purified with RNA purification kits (New England Biolabs) before analyzed by high performance liquid chromatography (HPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μl) were combined with 24 μl T7 High Yield RNA Synthesis Kit (NEB, Cat #E2040) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... fragmented and processed into an Illumina sequencing library using the NEB Ultra II FS kit (NEB cat. no. E7805S). Libraries were paired-end sequenced on an Illumina HiSeq 4000 instrument.
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified using Monarch PCR and DNA Cleanup kit (New England Biolabs, Inc., Ipswich, MA, USA) and DNA concentration was quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The S358A mutation was introduced into the STING ORF in this construct using Q5 site-directed mutagenesis kit (NEB). Virus generation from these constructs and cell transduction were as described (Sidorova et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... m6A pulldown was performed with a rabbit monoclonal anti-m6A antibody in the EpiMark N6-Methyladenosine Enrichment Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Cell Biology 2022Quote: IP samples were prepared for high-throughput sequencing by using the Ultra II DNA Library Prep kit (NEB E7645L). Samples were multiplexed and paired-end sequencing was performed using a MiniSeq (Illumina ...
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bioRxiv - Genomics 2022Quote: ... sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, MA). Library preparation and sequencing were performed at the University of Missouri Genomics Technology Core ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Intact total RNA was reverse transcribed into cDNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) according to manufacturer’s instructions with random primers ...
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bioRxiv - Genomics 2020Quote: ... libraries were quantified by qPCR using either the NEBNext® Library Quant kit (New England Biolabs, Cat. N: E7630S) or the KAPA Library Quantification Kit (scRNA-seq libraries only ...
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bioRxiv - Genomics 2019Quote: ... Strand-specific sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries from two biological replicates were sequenced on the Illumina NextSeq 500 platform resulting in 22-25×106 single-end reads ...
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bioRxiv - Genomics 2020Quote: ... RT adapters (supplied in the kit) were ligated to the RNA strand using T4 DNA ligase (New England Biolabs). Purification steps were performed using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: Micro-C sequencing library was generated by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) with some modifications ...
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bioRxiv - Genomics 2020Quote: ... Sequencing library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit (NEB, Ipswich, USA). Libraries (350 bp insert size ...
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bioRxiv - Immunology 2021Quote: ... Dephosphorylation was performed on the respective µRNA with addition of 5µg to a Quick dephosphorylation kit (New England BioLabs). Dephosphorylated µRNA was again purified using µRNA isolation columns ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7560S, E7580, New England Biolabs) with the customisation of a dilution of primers 1:2 ...
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bioRxiv - Molecular Biology 2021Quote: ... in vitro transcription was performed to produce sgRNAs using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Next ...
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bioRxiv - Biochemistry 2019Quote: ... The point mutation of loa22 variants were obtained by using Q5® Site-Directed Mutagenesis Kit (NEB, Ipswich, MA) with their relevant primers ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from the cocktail culture by Monarch Total RNA Miniprep Kit including DNase I (NEB, Ipswich, MA) which was confirmed by multiplex RT-PCR and gel electrophoresis ...
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bioRxiv - Microbiology 2019Quote: Additional mutant variants were generated with the oligonucleotides listed in Table S7 and using a protocol that adhered to the manufacturer’s instructions of the Q5 SDM kit (NEB). The protocol contained several modifications ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Genomics 2020Quote: ... 500ng of total RNA was subjected to ribosomal-RNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Qualified DNA were interrupted into fragments and constructed to library using Next Ultra DNA Library Prep Kit (NEB, USA), then sequenced on the Illumina plantform ...
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bioRxiv - Molecular Biology 2019Quote: ... we followed the manufacturer’s protocol for the NEBNext® Ultra™ II Directional RNA Library Prep kit (NEB, E7765S) and poly(A ...
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bioRxiv - Genomics 2019Quote: ... 16 libraries for each histone mark) using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, cat. # E7645). NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment (sense/anti-sense) Pceh-23_L::acy-2 fragment(sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Developmental Biology 2019Quote: ... libraries were generated using the NEBNext Ultra II library preparation kit with 15 cycles of PCR amplification (NEB E7645). The libraries for the ‘MicroChIP’ samples were generated using the MicroPlex library prep kit (Diagenode C05010012 ...
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bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Developmental Biology 2020Quote: ... into p328-attB-ubi:EGFP (gifted from the Piotrowski Lab at Stowers Institute) (Mosimann et al., 2011) via NEBuilder HiFi assembly kit (NEB) using PCR products from p328 plasmid amplified with pDest-gibson_FW/RV primers (Table S1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Point mutations were introduced by Q5 site directed mutagenesis kit according to manufacture protocol (NEB, Ispwich, MA 01938, USA). All constructs were confirmed by DNA sequencing ...
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bioRxiv - Genetics 2019Quote: ... Mutant transgenes were generated by site-directed mutagenesis of SPD771 using the Q5 Site-Directed Mutagenesis kit (NEB E0554S) and primers cdr-1p_delGATA1F (CCCTACTTTCccgctgCATTATGTCATCGGG ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and RT-qPCR was performed using the Luna Universal Onestep RT-qPCR kit (New England Biolabs, Ipswich, Massachusetts, USA) on a Bio-Rad CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories Inc. ...
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bioRxiv - Physiology 2020Quote: ... cDNA was then synthesized from 1μg RNA template through reverse transcription using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs), as recommended by manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The sequencing library was constructed according to NEBNext Ultra II DNA library Prep kit for Illumina (NEB, cat#7645L), NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Genetics 2019Quote: RNA libraries were prepared using the NEBNext Ultra Directional RNA Library preparation kit (New England Biolabs, Ipswich, MA, USA) and sequenced on the Illumina NextSeq 500 ...
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bioRxiv - Biochemistry 2020Quote: ... and RNAse free water and lastly 1.5 μL of T7 RNA polymerase (HiScribe T7 In Vitro Transcription Kit from New England Biolabs or AmpliScribe T7 High Yield Transcription Kit from Epicenter) ...
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bioRxiv - Microbiology 2020Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After DNA quantification using Qubit dsDNA HS Assay Kit and Qubit 2.0 fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... The template was then used for in-vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB). The in-vitro transcription reaction was carried out at 37°C for 16 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... Pooled PCR products (1 μg) were digested with the NEBNext dsDNA fragmentase kit (New England BioLabs, Inc., Ipswich, MA). Fragmented DNA was end-repaired with the NEBNext End Repair Module (New England BioLabs ...
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bioRxiv - Biochemistry 2019Quote: ... PCR amplified ORF was cloned into a pGEX4T1 vector using NEB HF cloning kit (New England Biolabs, Inc. USA). E69A-TRAF6 was cloned using PCR based site-directed mutagenesis ...
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bioRxiv - Genetics 2019Quote: ... the transcriptome library was constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Furthermore ...
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bioRxiv - Cancer Biology 2019Quote: A deletion between amino acid 336 and the beginning of GFP was generated from the MCSV:GPR68-GFP plasmid using the Q5 mutagenesis kit (NEB).
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bioRxiv - Microbiology 2019Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Microbiology 2019Quote: ... Shotgun metagenomic libraries were prepared using New England BioLabs Ultra FS II library kits (New England BioLabs, Ipswich, MA), ligated to iTru adapter stubs (18) ...
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bioRxiv - Cancer Biology 2020Quote: ... SALL4-targeting sgRNA candidates were transcribed with HiScribe™ Quick T7 High Yield RNA Synthesis Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: RNA sequencing libraries were prepared for independent replicates of mRNA samples by using NEBNext Ultra RNA Library Prep Kit for Illumina according to manufacturer’s instructions (New England Biolabs). Briefly ...