Labshake search
Citations for New England Biolabs :
5151 - 5200 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Substrate ssRNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, Ipswich, MA, SUA) and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: ... the PCR amplicons were purified used the Monarch PCR and DNA Cleanup Kit (New England Biolabs, Whitby, Canada) and sent for Sanger sequencing at the McMaster Genomics facility ...
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bioRxiv - Microbiology 2024Quote: ... The library preparation was elaborated using the NEBNext® SingleCell/Low Input cDNA Synthesis kit (New England Biolabs). The prepared libraries were then sequenced ...
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bioRxiv - Immunology 2024Quote: ... DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (cat. E7645S, New England Biolabs) and purified with AMPure SPRI beads (cat ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA from SH-SY5Y cells and iPSCs was extracted with the Monarch Genomic DNA Purification Kit (NEB). PCR was performed with Q5 Hot Start High-Fidelity Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... cells from a transfected well were used for RNA extraction using a Monarch total RNA miniprep kit (NEB). Isolated RNA was subjected to denaturing gel electrophoresis alongside a molecular weight ladder (Dynamarker prestain marker for RNA high ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng of total RNA underwent ribosomal depletion with the NEBNext rRNA Depletion Kit (Bacteria) (NEB catalog # E7850) and library preparation with the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202 ...
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bioRxiv - Synthetic Biology 2021Quote: ... total DNA from one clone was isolated using the Monarch Kit for HMW DNA Extraction from Bacteria (NEB#T3060) and confirmed by Nanopore sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol using Agilent Mx3000P instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated using Invitrogen PureLink RNA Mini Kit following manufacturer’s instructions and treated with DNase (New England Biolabs). Biological triplicate samples were submitted to DNA Technologies and Expression Analysis Core at UC Davis Genome Center for the generation of 3’Tag RNA-Seq libraries ...
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bioRxiv - Microbiology 2020Quote: Site-directed mutagenesis was performed on pBPVP1456 with a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and primers listed in Table 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-Seq libraries (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, New England BioLabs, Catalog No. E7760) were produced from 16 samples according to the manufacturer’s protocol using 200 ng total RNA ...
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bioRxiv - Cell Biology 2020Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... addition of a N-terminal Hemagglutinin (HA) epitope was done using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following manufacturer recommendations.
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bioRxiv - Immunology 2021Quote: ... Devil CIITA was then cloned into SfiI-digested pSBbi-BH using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB). The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified using the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) and US CDC real-time RT-PCR primer/probe sets for 2019-nCoV_N1 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Genetics 2021Quote: ... and ligated with the amplicons Uro promoter and GeneSwitch using NEBuilder HiFi DNA Assembly Kit (NEW ENGLAND BioLabs, E2621X). Transgenic lines were generated using standard methods for P-element-mediated germline transformation (BestGene Inc).
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Genetics 2021Quote: ... High molecular weight gDNA extraction using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs, T3060L) is performed on tissues isolated from N1 or higher generation mice ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) according to protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA library was prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England BioLabs) with 5cycles of pre-amplification and 3 to 7 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... and then a cDNA library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L). The libraries were checked for quality using the Fragment Analyzer NGS Analysis DNF-474 kit (Advanced Analytical ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific libraries were created using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Hitchin, Herts, UK) and 20ng of the rRNA depleted RNA.
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted with Trizol and ribosomal RNA was removed using a NEBNext rRNA depletion kit (New England BioLabs), according to manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... was used to generate libraries with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Cat# 7645). The resulting libraries were sequenced (single end 50 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... The library preparation was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S). The libraries were multiplexed (six samples per lane ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA-seq libraries were prepared using Total RNA-seq ScriptSeq Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol23 for improving low-input samples and sequenced with NextSeq500 (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... And sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). mRNA was selected by purifying polyA+ transcripts (NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Kit (New England Biolabs, Massachusetts, USA) with a modified protocol to account for fragmented DNA ...
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bioRxiv - Microbiology 2021Quote: ... and adapter ligation reactions were performed on the fragmented DNA using the NEBNext Ultra II kit (New England Biolabs). Illumina paired-end sequencing was performed on a NextSeq500 device at the Georgia Genomics Facility (University of Georgia).
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bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were constructed with the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries were generated using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Genomics 2020Quote: ... Then paired-end libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and 3Gb paired-end reads of 150bp were produced for each library.
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bioRxiv - Microbiology 2022Quote: ... we first generated two plasmids encoding guide RNAs targeting either MMCAP2_0582 or MMCAP2_0575 by modification of the base plasmid p426-SNR52p-gRNA.Y-SUP4t (25) using the Q5 Site-Directed Mutagenesis kit (NEB). In addition ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs #E7805) and Multiplex Oligos for Illumina (NEB #E7500) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for mRNA purification followed by a NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Massachusetts, U.S.) and sequencing was carried on an Illumina® NovaSeq™ 6000 platform ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was reverse-transcribed according to the manufacturer’s instructions using the ProtoScript Strand cDNA Synthesis kit (New England Biolabs) as previously described (Nantie et al. ...
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bioRxiv - Neuroscience 2021Quote: RNAs were in vitro transcribed from PspOMI-linearized pcDNA3.1(+) reporter plasmids using HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA ...
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bioRxiv - Molecular Biology 2021Quote: Libraries for next-generation sequencing were prepared using the NEBNext Ultra II DNA library prep kit (New England Biolabs) following the manufacturer’s instructions and sequenced on an Illumina NextSeq 500 platform (75 bp reads ...
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bioRxiv - Molecular Biology 2020Quote: EM-seq library construction was performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions with modifications ...