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Citations for New England Biolabs :
4901 - 4950 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... histolytica RNA was prepared using the Monarch Total RNA Miniprep Kit (NEW ENGLAND BioLabs, Ornat, Nes Ziona, Israel). According to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... and RNAseq libraries were prepared using the NEB Ultra II Directional RNA library Prep kit (New England Biolabs), with 1 ug total RNA input and initial poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 uL of Quick Ligation Buffer and 5 uL of Quick T4 Ligase (NEB Quick Ligation Kit, M2200S). The ligation reaction was incubated for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was then amplified by performing a multiplexed PCR in two pools using the ARTIC-N5 primers and the Q5 Hot Start DNA polymerase (New England BioLabs ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instruction and further purified using a Monarch® RNA Cleanup Kit (50 μg) (NEB). RNA integrity was monitored with agarose gels ...
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bioRxiv - Microbiology 2022Quote: ... A sequencing library was generated using the NEB Next® Ultra™ DNA Library Prep Kit (NEB, USA) following the manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, US-CA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Systems Biology 2024Quote: ... 400 ng RNA were used as input for NEBNext Ultra RNA Library Prep Kit (New England Biolabs, USA). One sample (nonlesional skin of Pat1 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-Seq libraries were prepared by NebNext Ultra II RNA library preparation kit (New England Biolabs, Massachusetts, USA) and sequenced using Illumina’s state-of-the-art NovaSeq 6000 V1.5 platform (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were completed with the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina” (NEB #E7805) and subsequently analysed on an Illumina instrument by following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Developmental Biology 2024Quote: ... The sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB Catalog# E7645S) following manufacturer instructions.
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB). Pooled libraries were sequenced with paired end sequencing on a Novoseq 6000 by Novogene.
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (New England BioLabs, #E7645S) per manufacturer’s instructions with minor modifications ...
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Physiology 2024Quote: RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s polyA mRNA workflow at the Advanced Analysis Center at the University of Guelph (Guelph ...
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bioRxiv - Neuroscience 2024Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2024Quote: ... and further cleaning (to get rid of DNA contamination) with Monarch® Total RNA Miniprep Kit (NEB, T2010S) by following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Ribosomal RNA was removed with NEB Ribo-Zero Plus rRNA Depletion Kit (New England Biolabs, Cat No. 20037135) prior to RNA-seq library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB #E7765).
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bioRxiv - Genetics 2024Quote: ... RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation ...
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bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
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bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were extracted and assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The assembly product was transformed into E ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II FS Library Prep Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s instructions with inserts of approximately 350 bp ...
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bioRxiv - Genomics 2024Quote: Hadrurus arizonensis DNA was extracted using the MonarchⓇHigh Molecular Weight (HMW) DNA Extraction Kit: Tissue Protocol (NEB, T3060). Changes made to the protocol include ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microlitre reaction mixtures were prepared using the Luna® Universal qPCR Master Mix kit (New England Biolabs) as follows ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was either extracted with phenol-chloroform followed by Monarch Total RNA Miniprep kit (New England Biolabs) or with the Quick-RNA Miniprep kit (Zymo) ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
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bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Sequencing of the library was performed using Illumina HiSeq2500 and NextSeq2000 to produce 65-bp single-end reads and 36-bp paired-end reads ...
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bioRxiv - Cancer Biology 2024Quote: ... and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were pooled and single-end sequenced (1×75 bp ...
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bioRxiv - Microbiology 2024Quote: ... mApple was cloned in place of the eYFP gene using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and BstXI and NotI restriction sites as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the specific product was purified from the agarose gel using the Monarch DNA Gel Extraction Kit (NEB, T1020S). Each reverse primer contains the T3 RNA Polymerase minimal promoter sequence (shown in bold and underlined in Table 1) ...
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 3.) Library preparation was performed using the NEBNext Ultra II library preparation kit for Illumina (New England Biolabs) and 4. ...
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bioRxiv - Developmental Biology 2024Quote: ... Template pDNA was linearized and ARCA-capped IVT-mRNA was produced using the T7 HiScribe kit from NEB, pseudo-uridine was incorporated into the reaction ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...