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Citations for New England Biolabs :
5251 - 5300 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... A Gibson assembly was carried out to link all DNA fragments and assemble the final integrative pHO_pCMV_CggR_ble plasmid using the NEB Gibson assembly kit (New England Biolabs, MA, US). 5 µl of reaction mix was transformed into chemical competent E.coli DH5alpha cells ...
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bioRxiv - Physiology 2021Quote: ... The biotinylated peptides were placed on streptavidin-coated plates and three rounds of solid-phase panning were conducted using 109 random 12-mer peptides fused to a minor coat protein of M13 (pIII) following the manufacture’s protocol (Ph.D-12 Phage Display Peptide Library Kit, NEB, E8110S). Sequenced data were subsequently analyzed as follows ...
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bioRxiv - Genomics 2021Quote: ... cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB#E7760S) for 50ng of total RNA and using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420 ...
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bioRxiv - Genomics 2021Quote: ... for 50ng of total RNA and using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420) for 100ng of WT1 ...
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bioRxiv - Genomics 2019Quote: ... Sonicated DNA was used to construct libraries with a NEBNext Ultra DNA Library Prep Kit (NEB, Ipswich, MA, USA), and sequenced on an Illumina MiSeq instrument.
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... indexed ChIP-seq libraries were constructed using the NEBNext Ultra II Library Prep Kit for Illumina (New England Biolabs) using the application note ‘Low input ChIP-seq’ ...
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bioRxiv - Genomics 2021Quote: ... and were excised between 300 bp - 900 bp and cleaned using the Monarch DNA Gel Extraction Kit (NEB, T1020). The libraries were quantified and qualified using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent ...
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bioRxiv - Genomics 2021Quote: ... DNA library preparation was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, #E7370) with 10 ng input ...
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bioRxiv - Genomics 2021Quote: ... DNA library preparation was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, E7370) with 10 ng input ...
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bioRxiv - Microbiology 2020Quote: ... 16S amplicon from all samples was prepared for sequencing using the NEBNext Illumina library preparation kit (New England Biolabs), and sequenced with 400 forward cycles of v2 Miseq chemistry.
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Systems Biology 2020Quote: ... we prepared sequencing libraries using the NEBNext single-cell/low-input RNA sequencing library preparation kit for Illumina (NEB) then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed a 10-cycle PCR using a Phusion PCR Kit according to the manufacturer’s protocol (New England Biolabs) with multiplexed primers and adjusted PCR products to 10 μM for sequencing on either an Illumina HiSeq2500 (125 bp paired-end ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was once again purified with a Qiagen MinElute kit and dA-tailed with 11.25U Klenow fragment (exo-) enzyme (NEB). DNA fragments were then selected for size (200-300bp ...
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bioRxiv - Genomics 2021Quote: ... cDNAs were generated using the ProtoScript II First Strand cDNA Synthesis Kit and Oligo(dT) primers (New England Biolabs). RT-qPCR were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Genomics 2021Quote: ... After determining that some whole fly libraries made using NEBNext Ultra-II DNA library prep kit for Illumina (NEB) were as complete and has better yields than the then discontinued Nextera kit ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, USA) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... clr4 gene specific sgRNA was cloned into Cas9 containing pLSB-KAN plasmid by Golden Gate Assembly kit (E1601S, NEB). The resulting plasmid clr4-pLSB-KAN and clr4 HR template obtained by annealing primer pair WW748-clr4-HR-F/WW749-clr4-HR-R (Table S3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The 36N plasmid DNA library was generated using a Q5 site directed mutagenesis kit (New England Biolabs, Ipswitch, MA). For amplification ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs, MA, USA), and shotgun sequenced by Illumina-MiSeq at the Pittsburgh Bacteriophage Institute ...
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bioRxiv - Biochemistry 2021Quote: ... while the RBD constructs were introduced in frame to the mu phosphatase signal sequence and incorporated within pcDNA3.1 via Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... each 180 μl sample/Saccharibacteria co-culture was treated with Endo Hf Kit P0703L (New England Biolabs, Evry, France): 3 μl of each reagent was added to the sample and incubated for one hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pES101 (encoding GPx7) was constructed by assembling three DNA fragments using the Gibson Assembly Kit (New England Biolabs). The DNA fragments used were a fragment amplified from pcDNA3.1+ (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA libraries were then generated by NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). A custom-designed probe panel (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... and then cloned into the lentiviral expression vectors coexpressing a puromycin resistance gene or GFP (pLX_TRC307 and pLX_TRC312 from GPP) using a Gibson Assembly Cloning Kit (New England Biolabs E5510S). dTAG expression vector and dTAGV-1 compound were kindly provided by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... The pDD162 plasmid was used to deliver Cas9 and sgRNAs modified using the Q5 Site-Directed Mutagenesis Kit (NEB) for sequence specific CRISPR editing of each gene ...
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bioRxiv - Cell Biology 2021Quote: CFTR-Δ23 and CFTR-W1282X were created from the synthetic CFTR high codon adaption index (HCAI) construct subcloned in the pcDNA3.1/Neo(+) vector (73) using the Q5 Site-Directed Mutagenesis Kit (NEB) with primers flanking each exon (Table S1) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was prepared by using the First Strand cDNA Synthesis Kit following the manufacturer’s instructions (New England BioLabs). The same PCR program was applied to the total RNA sample after DNase treatment as negative controls.
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bioRxiv - Microbiology 2020Quote: ... and OC43) viral RNA genomes were synthesized using the LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). The reaction mixture had a volume of 20◻μL and comprised of 4◻μL of 5X LunaScript RT supermix ...
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bioRxiv - Systems Biology 2021Quote: ... all DNA samples were prepared for Illumina sequencing using the NEBNext Ultra DNA Library Prep Kit (NEB product #E7370), with either single index or dual index primers also obtained from NEB ...
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bioRxiv - Systems Biology 2021Quote: ... and RNA-seq libraries were prepared with NEB-Next Ultra II Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced on an Illumina NovaSeq ...
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bioRxiv - Systems Biology 2020Quote: ... and library preparation were performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 3μg of RNA was used for library generation uding NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). The library preparations were sequences on an Illumina Hi-Seq platform and 125bp/150bp paired-end reads were generated ...
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All-Flesh Tomato Regulated by Reduced Dosage of AFF Provides New Insights into Berry Fruit EvolutionbioRxiv - Plant Biology 2021Quote: ... Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Sorted transduced cells were frozen down for later use or subjected to EnGen mutation detection kit (New England BioLabs) for on-target gene editing confirmation ...
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bioRxiv - Microbiology 2021Quote: ... and fragment libraries prepared using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, USA). Metagenomic libraries were sequenced on the Illumina NovaSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... DNA was prepared for sequencing using the NEBnext UltraTM II DNA library preparation kit for Illumina (New England Biolabs). Sequence data were generated on the Illumina HiSeq 2500 next generation sequencing system with the 2 x 250 bp paired end mode ...
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bioRxiv - Genomics 2021Quote: RNA libraries were prepared using an NEBNext Single Cell/ Low-Input RNA Library kit (New England BioLabs, Tokyo, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR on extracted RNA was performed using the Luna One-Step RT-qPCR Kit (M300, New England Biolabs). The samples were analyzed using a LightCycler 480 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... using the IP4 set of primers and probe as described on the WHO website (https://www.who.int/docs/default-34source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-35pasteur-paris.pdf?sfvrsn=3662fcb6_2) and the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, France).
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized using 200ng total RNA with the ProtoScript® First Strand cDNA Synthesis kit (New England Biolabs) utilizing random hexamers ...
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bioRxiv - Microbiology 2020Quote: ... The USP13 C345A mutant was created using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs Inc, MA, USA) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... all PCR products were purified using the Monarch® PCR and DNA Clean-up Kit (New England Biolabs, USA) following the manufacturer’s instructions and eluted in molecular grade water.
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bioRxiv - Microbiology 2021Quote: ... Library preparation was achieved with a NEBNext Ultra II DNA Lib Prep Kit (New England Biolabs, Ipswich, MA, USA) in accordance with protocols provided by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: The 6K1:GFP constructs and its derivatives were produced using the Gibson cloning kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat #E7760) following polyA RNA enrichment (NEB cat #E7490 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... pEGFP-C1 or pcDNA5/FRT/TO-N-TAP with gateway cloning using the SP6-CHIKV-replicon-SG-GLuc as a template. Mutants (except for replicon mutants, see above) were generated using standard mutagenesis procedures (e.g. Q5 mutagenesis kit (NEB)) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... SET8 domains as well as point mutations were performed using Q5 site-directed mutagenesis kit (New England Biolabs # E0554S). Primers used for cloning and mutagenesis are available upon request.