Labshake search
Citations for Illumina :
4651 - 4700 of 5691 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The V4 region of the 16S rRNA gene was amplified from extracted fecal DNA and sequenced on a MiSeq (Illumina, San Diego, CA) using 2 x 250 V2 chemistry following the MiSeq Wet Lab SOP [(22) ...
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bioRxiv - Cell Biology 2022Quote: ... using poly-T oligos attached to magnetic beads and converted to sequence-ready libraries using the TruSeq stranded mRNA sample prep protocol (cat. #: RS-122-2101, Illumina, San Diego, CA). The final libraries were validated on the bioanalyzer DNA chips ...
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bioRxiv - Microbiology 2020Quote: ... The genome was sequenced with Illumina technology using a GAIIx sequencing platform on paired-end libraries prepared with the Nextera XT DNA Kit (Illumina, San Diego, CA). A total of 84 contigs (0.5 to 330 kb ...
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bioRxiv - Developmental Biology 2021Quote: ... Next generation sequencing of equimolar pools of cDNA libraries was performed by loading a paired-end 75bp rapid flow cell to generate a minimum of 25M raw reads on a HiSeq 2500 sequencing platform (Illumina, San Diego, CA). Raw sequencing data (submitted to Gene Expression Omnibus ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were pooled at a concentration of 5nM and run on an Illumina HI-SEQ 2500 sequencer (Illumina, Inc. San Diego, CA) to obtain paired-end reads of 75 bases (PE75) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing of the 16S rRNA gene amplicons was conducted on the Illumina Mi-Seq DNA sequencing platform (Illumina Inc., San Diego, CA, U.S.A.). Amplicon sequence data was analyzed using an internal pipeline known as the Amplicon Sequencing Analysis Pipeline (Zhang et al. ...
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bioRxiv - Microbiology 2021Quote: ... Single-end reads with a length of 75 bp were sequenced on a NextSeq 500 Sequencing System instrument (Illumina, San Diego, CA, USA) with a sequencing depth of 100 million reads per sample.
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bioRxiv - Neuroscience 2021Quote: Library preparation and sequencing of the samples were conducted at the Mayo Clinic Genome Analysis Core using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA). The library was sequenced on Illumina HiSeq2000 instruments ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries for short- and long-read sequencing were generated with the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA) and the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Cell Biology 2020Quote: ... RNA sequencing was performed by the Genomics Sequencing Center at Virginia Tech using an mRNA-stranded Seq library prep (Illumina, San Diego, CA) and NextSeq High Output 75SR sequencing (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Individual libraries were constructed from some of the strain DNA preparations described above using an Illumina Nextera XT DNA sample preparation kits with appropriate indices tags according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). The libraries were pooled together and run on an Illumina MiSeq DNA sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: The cDNA synthesis and library construction were done using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Messenger RNA capture based libraries were prepared starting from 8.5 µL DNase treated and spike-in supplemented RNA eluate using the TruSeq RNA Exome Library Prep Kit (Illumina, San Diego, CA, USA). Each sample underwent individual enrichment according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... is a 30-gene NGS test for somatic mutations in myeloid neoplasms using the Truseq Amplicon Cancer Panel kit (Illumina, San Diego, CA). It contains amplicons for hot-spot locations of the following genes ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and single end-sequenced (1×75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina, San Diego, CA). Quality control was performed using Fastqc (v0.11.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to cDNA library making (fragmentation, first and second strand syntheses, adenylation, ligation and amplification) by TruSeq Stranded mRNA Library Prep (#20020594, Illumina, San Diego, CA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Illumina TruSEQ RNA library prep and sequencing reagents were used following the manufacturer’s recommendations using polyA-selected transcripts (Illumina, San Diego, CA, USA). The samples were sequenced on the Illumina NovaSeq platform (2 x 150 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... libraries were prepared using the Quant-seq 3’ mRNA Library Prep Kit FWD (Lexogen, Greenland, NH) and single-end 75bp reads were sequenced on the NextSeq 500 (Illumina, San Diego, CA). Genes queried for hiPSC identity were listed on the TaqMan hPSC Scorecard Assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... libraries were prepared at the UConn Center for Genome Innovation using the Illumina Strand mRNA Kit and 100bp paired-ends reads were sequenced to an average depth pf 40 million reads/replicate on the NovaSeq (Illumina, San Diego, CA).
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bioRxiv - Neuroscience 2022Quote: ... of the three offspring and their parents was performed at the University of Washington with 1 μg of DNA on a HiSeq 2000 Sequencing System (Illumina, San Diego, CA). The WGS data were aligned using a standard BWA pipeline to the Budgerigar (Melopsittacus undulatus ...
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bioRxiv - Molecular Biology 2022Quote: Pooled libraries were subjected to 150 bp paired-end sequencing according to the manufacturer’s protocol (NovaSeq 6000, Illumina, Inc., San Diego, CA, USA). Bcl2fastq2 Conversion Software (Illumina Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... RIP-seq libraries were pooled and sequenced on a MiSeq 50 base pair single-read flow cell using v2 reagents (Illumina, San Diego, CA). SIR libraries were also pooled and sequenced on either a HiSeq2500 high output 100 base pair paired-end flow cell using TruSeq SBS v3 reagents or on a NextSeq500 high output 75 base pair single-read flow cell using v2 reagents (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... SIR libraries were also pooled and sequenced on either a HiSeq2500 high output 100 base pair paired-end flow cell using TruSeq SBS v3 reagents or on a NextSeq500 high output 75 base pair single-read flow cell using v2 reagents (Illumina, San Diego, CA).
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the New England Biolabs NEBNext rRNA depletion kit (Bacteria) and NEBNext Ultra II Directional RNA library prep kit (Illumina, San Diego, CA). The libraries were quantified by KAPA qPCR library quantitation assays and sequenced paired-end using MiSeq platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... a sequencing library of the V3-V4 region of the 16S rRNA gene was constructed following the manufacturer’s instructions (Part # 15044223Rev.B; Illumina Inc., San Diego, CA, USA) with improvement as previously described [20] ...
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bioRxiv - Microbiology 2022Quote: ... A sequencing library of the V3-V4 region of the 16S rRNA gene was constructed following the manufacturer’s instructions (Part # 15044223Rev.B; Illumina Inc., San Diego, CA, USA) with improvement as previously described [20] ...
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bioRxiv - Synthetic Biology 2022Quote: ... These libraries with different indexes were multiplexed and loaded on an Illumina NovaSeq instrument according to manufacturer’s instructions (Illumina, San Diego, CA, USA). Sequencing was carried out using a 150 bp paired-end (PE ...
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bioRxiv - Neuroscience 2020Quote: ... Next generation sequencing and bioinformatics analysis was performed by the National Genomics Infrastructure (NGI) at the Science for Life Laboratory on the HiSeq 2500 System using the HiSeq Rapid SBS Kit v2 (Illumina, San Diego, CA) generating >13.5 M reads/sample ...
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bioRxiv - Genetics 2022Quote: ... Libraries were constructed using the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen, Greenland, NH) and sequenced on an Illumina NextSeq 500 producing 85 nt single-end reads (Illumina, San Diego, CA) with each plate being split in half and each half being sequenced on a single lane to achieve desirable coverage.
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bioRxiv - Genomics 2022Quote: ... and the library pool was amplified via qPCR and loaded on one lane of a NovaSeq 6000 S4 flowcell (Illumina, San Diego, CA), where it was sequenced for 28 nt from one end and 151 nt from the other end ...
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bioRxiv - Immunology 2022Quote: ... The amplicons were quantified using KAPA Library Quantitation kit (KAPA, Cape Town, South Africa) and an equimolar amount of each sample was sequenced on the MiSeq System (Illumina, San Diego, CA) with 2×150 paired-end run parameters ...
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bioRxiv - Microbiology 2022Quote: ... Twenty percent of denatured PhiX was added to the amplicon pool (12 pM) and sequenced on the MiSeq platform using 300+300 bp paired-end V3 chemistry (Illumina, San Diego, CA). Raw sequence files were deposited to the NCBI Sequence Read Archive under the BioProject accession number ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We sent 100 ng of DNA from 289 sapsuckers (Supplemental Materials Table 1) to Genome Quebec for sequencing on either an Illumina HiSeq4000 PE150 or the NovaSeqSP 6000 PE150 (Table S1) (Illumina, San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... The RNA-Seq library was generated following the standard Illumina RNA-Seq protocol and sequenced on an Illumina NovaSeq 6000 machine (Illumina, San Diego, CA) obtaining an average of 40 million of single-end reads per sample ...
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bioRxiv - Genetics 2022Quote: ... Individual barcoded libraries were randomly multiplexed into groups of four samples using equimolar quantities and sequenced on a MiSeq using a v3 600 reagent kit (Illumina, San Diego, CA) to generate paired-end 250 bp reads ...
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bioRxiv - Genetics 2022Quote: ... Output total RNA was depleted for ribosomal RNA and prepped for RNA-seq using the Scriptseq Complete Gold (Epidemiology) kit (Illumina, San Diego, CA). A total of ~50 ng of ribosomal depleted RNA was used as an input to transcriptome libraries ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Two independent paired-end libraries were constructed per population from these DNA pools using the Genomic DNA Sample Preparation Kit (Illumina, San Diego, CA) (technical replicates) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-Seq libraries preparation and sequencing was performed by the IGA Technology Services (Udine, Italy) using the Illumina TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and one sample of each clonal population) were multiplexed into one pool and sequenced on Illumina HiSeq2000 instrument (Illumina Inc., San Diego, CA). Adapter sequence was trimmed using Trimmomatic-0.30 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq libraries were generated using SENSE Total RNA-seq Library Prep Kit v2 (Lexogen, Vienna, Austria) following the manufacturer’s protocol and sequenced on NextSeq 500 (Illumina, San Diego, CA, USA) using single-end 75 bp chemistry.
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were prepared with an Illumina TruSeq Stranded Total RNA kit with Ribo-Zero Plant or the BrAD-Seq method37,38 and sequenced for 86 bp single end with an Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA). Data preprocessing was performed as follows ...
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bioRxiv - Genetics 2020Quote: ... F2 mice carrying the Col4a1+/Δex41 mutation and genotyped at the UCSF Genomic Core Facility with a commercial single nucleotide polymorphism (SNP) panel (Illumina, San Diego, CA) which contains 646 informative SNPs for (CAST X B6 ...
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bioRxiv - Genomics 2020Quote: ... Libraries were indexed and pooled into 12 sample sets and sequenced as 101 bp paired-end reads using Illumina HiSeq2500 (Illumina, San Diego, CA). Raw data have been submitted to GEO (http://ncbi.nlm.nih.gov/geo ...
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bioRxiv - Zoology 2021Quote: ... carried out library preparation (with a Ribo-Zero for rRNA removal) and sequencing using a NovaSeq6000 and S4 Flowcells (Illumina, CA, United States). Sequencing of the 18 adults across 3 different treatments yielded a total of 1.24 billion reads with an average of 68 million reads per sample (SEM ...
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bioRxiv - Plant Biology 2021Quote: AC and SlBES1-RNAi-8 fruits at breaker and red ripe stages were collected for total RNA extraction and Illumina MiSeq library was constructed as described by manufacturer’s instructions (Illumina, San Diego, CA, USA) and then sequenced with the Illumina Miseq platform.
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bioRxiv - Physiology 2020Quote: ... Library preparation was performed with 1μg total RNA using the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Zoology 2021Quote: ... The library was sequenced with 150 bp paired-end reads in one lane of an Illumina HiSeq X (Illumina, San Diego, CA, USA). The RAD-Seq data were analyzed using the Stacks program (version 2.52 ...
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bioRxiv - Microbiology 2020Quote: DNA libraries of virome DNA were prepared using the same methods as described for genome sequencing and were sequenced on an Illumina HiSeq 2500 sequencer with a HiSeq v4 SBS sequencing kit (Illumina, San Diego, CA) producing 2×160-bp paired-end reads ...
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bioRxiv - Microbiology 2020Quote: ... DNA libraries were pooled and sequenced on both the Illumina HiSeq5000 and HiSeq2500 and fastq files were generated from demultiplexed reads with bcl2fastq Conversion Software (Illumina, San Diego, CA). Reads were trimmed and assembled using the A5ud pipeline (74) ...
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA) ...