Labshake search
Citations for Illumina :
3751 - 3793 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from the discs and libraries for Illumina HiSeq2000 sequencing were generated using the TruSeq RNA Sample Preparation Kit (Illumina, catalog ID RS-122-2002). Reads were mapped using Bowtie2 v ...
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bioRxiv - Plant Biology 2021Quote: ... and pooled libraries of 20 barcoded samples were sent for pair-end sequencing using an Illumina HiSeqTM 2500 sequencing platform (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Genomics 2021Quote: ... we performed another secondary analysis between two groups of control samples (Leicester study controls vs. Ottawa and ATVB controls) representing two main enrichment kits (Illumina ICE vs. Agilent SureSelect kits). The statistical analysis was performed in R 3.3.3 (55) ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were prepared in three independent replicates and RNAseq was performed on a HiSeq 2500 (Illumina, single-end 50-bp SR mid output run) at the Life Sciences Core Facility (LaCTAD) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA purified from hippocampi of WT and Atf6b−/− mice (n=2 per group) was used to prepare RNA libraries using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA), with polyA selection for ribosomal RNA depletion ...
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bioRxiv - Genomics 2022Quote: ... A total of five samples (three biological replicates for each of five samples) were sequenced to generate paired-end reads using an Illumina HiSeqTM 2000 platform (Illumina Inc., San Diego, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Total RNAs from cultured mouse WT and CD38 KO astrocytes at 5 DIV were used for RNA library preparation using TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA), with polyA selection for ribosomal RNA depletion.
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bioRxiv - Microbiology 2021Quote: ... and gDNA mocks were included in each PCR plate and sequenced alongside the samples sequenced with an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, US) following manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2020Quote: ... the purified sample was used as the template for barcoding with Nextera XT Index Kit v2 (FC-131-1002 - Illumina Inc, San Diego, CA, USA). The finished libraries were normalized to 4 nM and pooled ...
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bioRxiv - Plant Biology 2021Quote: ... were randomly pooled (4 samples per pool) and grouped using the TruSeq Paired-End Reads Cluster Kit on the cBot platform (Illumina Inc., San Diego, CA, USA). The cDNA libraries were posteriorly sequenced on the Illumina Genome platform Analyzer IIx with a TruSeq kit with 36 cycles (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... performed libraries of all samples corresponding to DNA-associated RNA molecules and small RNA high-throughput sequencing (high-throughput sequencing on Illumina Hiseq 2500 or Illumina MiSeq). Totally ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was used for library preparation using the Illumina TruSeq™ Stranded mRNA LT Sample PrepKit according to the manufacturer’s instructions (Cat. No. RS-122-2101, Illumina Inc. San Diego, CA, USA). Library quality was checked with a 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... and mRNA-seq libraries were constructed and sequenced by the University of Missouri DNA Core Facility using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA) and the Illumina HiSeq and NextSeq platforms.
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bioRxiv - Microbiology 2022Quote: ... Fragmented and indexed samples were run on the sequencer using a Micro flow cell with the MiSeq Reagent Kit v2 (Illumina; 150-bp paired-end reads) following Illumina’s recommended denaturation and loading procedures ...
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bioRxiv - Neuroscience 2022Quote: ... Transcriptomic profile of individual BAT samples was performed using commercial RNA-sequencing kits (NEBNext mRNA Library Prep Master Mix and NEBNext Multiplex Oligos for Illumina, New England Biolabs, Ipswich, MA) and adapted according to previous descriptions [22] ...
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bioRxiv - Cell Biology 2022Quote: The libraries were generated using depleted RNA obtained from 1 μg of total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s protocol without further modifications ...
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bioRxiv - Genomics 2024Quote: ... Ltd (Hong Kong, China) for polyA selected RNA sequencing library construction using the TruSeq RNA Sample Prep Kit v2 (Illumina Cat. No. RS-122-2001), and 150 bp paired-end (PE ...
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bioRxiv - Genomics 2024Quote: ... Ltd (Hong Kong, China) for polyA selected RNA sequencing library construction using the TruSeq RNA Sample Prep Kit v2 (Illumina Cat. No. RS-122-2001), and 150 bp paired-end (PE ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented and indexed samples were run on the sequencer using a Micro flow cell with the MiSeq Reagent Kit v2 (Illumina; 150-bp paired-end reads) following Illumina’s recommended denaturation and loading procedures.
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bioRxiv - Plant Biology 2023Quote: ... with insert sizes ranging from 100 to 600 bp using a commercial kit (TruSeq stranded mRNA Sample Preparation Kit; Illumina Inc., San Diego, CA, USA). The libraries were sequenced for 100 bases using an Illumina Hiseq 2500 SE100 system.
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bioRxiv - Microbiology 2023Quote: ... The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (Tremblay et al. ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared using the TruSeq® RNA Sample Prep v2 kit with the IDT for Illumina DNA/RNA UD indexes (Illumina, San Diego, CA, USA). Purification and fragmentation of mRNA ...
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bioRxiv - Genomics 2024Quote: ... For each pool 16 GB (1 GB per sample) of 150 bp paired-end sequencing was performed on the Illumina NovaSeq 6000 platform (Illumina Inc., San Diego, CA, USA) by BaseClear B.V ...
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bioRxiv - Pathology 2024Quote: ... A detailed description of the database and how it is maintained has been published elsewhere.47 BioVU participant DNA samples were genotyped on a custom Illumina Multi-Ethnic Genotyping Array (MEGA-ex; Illumina Inc., San Diego, CA, USA). Quality control included excluding samples or variants with missingness rates above 2% ...
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bioRxiv - Microbiology 2024Quote: ... The different libraries were pooled according to the effective concentration and the target amount of data – 6 Gb raw data per sample – and were sequenced by the Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, USA). Paired-end 150bp reads were generated.
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bioRxiv - Genomics 2024Quote: ... DNA extracted from venous blood underwent whole-genome sequencing using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) and Illumina HiSeq 2000 or HiSeq X sequencer ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: DNA methylation profiling of all PanNEN cohort samples was performed with 200–500ng of DNA using the Infinium® MethylationEPIC BeadChip array (850K; Illumina, Inc., San Diego, CA, USA) according to the protocols provided by the manufacturer.
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bioRxiv - Immunology 2021Quote: ... GEX libraries were pooled and sequenced at a depth of approximately 540,000,000 reads per sample in a single S4 flow cell and ADT libraries at a depth of approximately 79,000,000 reads per sample in a single lane of an S4 flow cell on a NovaSeq™ 6000 (Illumina, San Diego, CA; Extended Data Table 6)
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bioRxiv - Plant Biology 2022Quote: ... The dual index sequencing library was prepared using a Nextera™ XT DNA Sample Preparation Kit and Nextera™ XT Index Kit (Illumina, San Diego, CA, USA), both according to the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for each sample were prepared from ≥200 ng of sheared DNA with TruSeq® Nano DNA High Throughput Library Prep Kit (20015965, Illumina Inc., San Diego, CA, USA) and IDT for Illumina – TruSeq® DNA UD Indexes v1 (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to ensure sufficient RNA of high quality in each sample and prepared libraries using the Illumina TruSeq® mRNA Stranded Kit (Product#: RS-122-2101, Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122). We then used the Qubit Fluorometer to quantify the amount of cDNA in each sample prior to sequencing.
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bioRxiv - Neuroscience 2020Quote: ... the nuclei-containing pellet was resuspended with 50 µl of the transposase reaction mix which contained 25 µl of the 2xTD reaction buffer and 2.5 µl of Tn5 Transposase (Nextera DNA Library Preparation Kit, Illumina, FC-121-1030; note: these reagents are now available in the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... The library preparation for bulk tumors and cell lines was generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2) according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... we prepared n=40 sequencing libraries from 1µg total RNA per sample using Illumina® Stranded mRNA Prep kits with unique 5′ and 3′ index adapter pairs (IDT for Illumina® RNA UD Indexes, Set A). Libraries were PCR amplified for 10 cycles ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of total RNA was used for polyA selection and Truseq library preparation according to the instructions provided by Illumina (TruSeq RNA Sample Prep Kit v.2), with 8 cycles of PCR ...
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bioRxiv - Microbiology 2021Quote: ... Nextera XT indices were used to generate libraries from 1 ng of cDNA material for each sample following the instructions provided by Illumina (Illumina FC-131-1096 and FC-131-2001). Library preparations were purified using AMPure XP beads and checked for quality using the Agilent Bioanalyzer at Stanford Functional Genomics Facility (SFGF) ...
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bioRxiv - Genetics 2020Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit – sets A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102). Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... US) and sequencing (Illumina HiSeq2500 by Illumina, San Diego, CA, US, 2 x 250 bp for peDNA samples and Illumina HiSeq3000, 2 x 150 bp for Filter DNA) was performed at the Max Planck Genome Centre Cologne (MP-GC).
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bioRxiv - Cancer Biology 2022Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...