Labshake search
Citations for Illumina :
3301 - 3350 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: The process of library construction involved the purification of messenger RNA from total RNA using magnetic beads attached to poly-T oligos (TruSeq RNA Sample Prep Kit v2, Illumina Inc.). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... The Illumina library was constructed according to Illumina’s TruSeq Nano DNA Sample Preparation protocol and sequenced on the Illumina HiSeq X at a read length of 150bp paired-end (Illumina, USA). The long reads generated from ONT sequencing was used to create the de novo genome assembly with Shasta 0.11.1 (Shafin et al. ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 25-30 million reads per sample were obtained using paired-end 100 pb read length (Illumina NovaSeq 6000). A quality control analysis of the RNA-seq raw data was performed with FastQC v0.12.0 (Babraham Bioinformatics ...
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bioRxiv - Genomics 2023Quote: ... A total of 4 µg of RNA per sample was subjected to rRNA depletion using the RiboZero Plant Leaf kit (Illumina, MRZPL1224) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... Strand-specific libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2), according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... We diluted libraries and sequenced an equimolar pool all samples on a NextSeq 500 using a 150 cycle mid output kit (Illumina, 20024904) using 28 cycles for read one to capture the 10X barcodes and UMIs ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAseq libraries were prepared from the same samples using TruSeq RNA Library Prep kit according to the manufacturer’s instructions (Illumina Part # 15026495). Sequencing data were analyzed using Bowtie v1.0.0 45 to remove rRNA reads ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries had been prepared using the TruSeq RNA Sample Preparation Kit v2 of Illumina and sequenced on the HiSeq 2500 platform (Illumina Inc) as 101 bp paired ends ...
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bioRxiv - Genomics 2023Quote: ... and Illumina PCR-free libraries with insert sizes of 350-bp were constructed using Truseq Nano DNA HT Sample preparation Kit (Illumina USA). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Samples were pooled and sequenced on an Illumina NextSeq 500 using paired-end (2×150bp) mode (Illumina, Inc., San Diego, CA).
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bioRxiv - Plant Biology 2023Quote: ... libraries were prepared from 2 µg of total RNA using the TruSeq RNA Sample Prep Kit (Illumina, Cat # FC- 122-1001) and sequenced on the HiSeq 4000 to produce at least 18 million 50 bp single-end reads.
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bioRxiv - Neuroscience 2023Quote: ... Bisulfite converted libraries were sequenced to 35 – 40 million PE-150×150bp clusters per samples using HiSeq 4000 Sequencer (Illumina, CA).
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bioRxiv - Microbiology 2023Quote: ... Fragmented and indexed samples were run on the sequencer using the MiSeq Reagent Kit v2 (Illumina; 250□bp paired-end reads) following Illumina’s recommended denaturation and loading procedures.
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bioRxiv - Biophysics 2023Quote: ... We performed 8 additional cycles of PCR with Nextera 24-Index kit for indexing before sample pooling (Illumina, FC-121-1011), for which we used 7.5 μl of the elute as template ...
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bioRxiv - Genomics 2023Quote: ... Samples were then loaded onto the Illumina NextSeq500 Instrument using a Mid-output 300 cycle kit (Illumina Catalogue FC-404-2003) or the MinION flow cell ONT instrument (R9.4.1) ...
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bioRxiv - Cancer Biology 2022Quote: Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (Illumina, cat# RS-122-2001), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and we constructed the directional RNA-seq libraries using Illumina’s TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA), so that multiplex sequencing can be achieved by using the barcoded PCR primers.
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bioRxiv - Systems Biology 2023Quote: ... was used for purification before sequencing was carried out with pooled samples in paired-end modus (PE300) on a MiSeq system (Illumina, Inc.) with 25% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... the amplicons from the set of samples were pooled and sequenced at an average depth of 23639 ± 6476 reads per sample using a MiSeq desktop sequencer (Illumina, USA). The sequencing datasets were processed following the bioinformatics procedure summarized by Karst et al ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of total RNA samples was used for library preparation for each sample using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina, USA). Sequencing was performed on Illumina’s NovaSeq6000 platform with cycling conditions to obtain 2x100bp reads on an S2 flowcell to obtain ∼70 million reads per sample ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples with RNA integrity number (RIN) > 8.0 were shipped to Beijing Genome Institute for library preparation and sequencing (Illumina HiSeq 2500). Around 70 million reads were obtained for each pooled sample ...
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bioRxiv - Genomics 2023Quote: Three samples of 50 ng each of genomic DNA were sheared and processed using the Nextera DNA Library Prep Kit (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and a duplicate unrelated control sample was diluted to 5 ng/µl in low TE provided in AmpliSeq Library PLUS (384 Reactions) kit (Illumina, 20019103). AmpliSeq was carried out following the manufacturer’s protocol (document 1000000036408v07) ...
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bioRxiv - Genomics 2023Quote: ... RNA libraries were prepared from 500 ng RNA samples using the TruSeq Stranded mRNA Kit (Illumina Inc., San Diego, CA, US) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Urine samples were then sequenced with (either the 2 x 150 bp or 2 x 75 bp configuration) to a mean depth of 30 M reads per sample on NextSeq 2000 (Illumina Inc); sequencing length did not impact gene detection efficiency (Supplementary Fig ...
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bioRxiv - Immunology 2023Quote: ... Single cell libraries were prepared from 10,000 cells per pooled sample and sequenced with the NextSeq1000 (Illumina, San Diego, CA, USA) at a depth of > 30,000 paired reads per cell ...
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bioRxiv - Microbiology 2023Quote: ... DNA libraries of each sample were prepared for whole genome sequencing (WGS) using a Nextera XT kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... A total of 4 µg of RNA per sample was subjected to rRNA depletion using the RiboZero Plant Leaf kit (Illumina, MRZPL1224) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (Illumina, cat# RS-122-2001), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... DNAse-treated RNA extracts were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was subjected to prepare libraries using Illumina TruSeq RNA and DNA Sample Prep Kits (Illumina, San Diego, CA, USA). Library qualities were confirmed using Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Index-coded samples were clustered on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, USA) and sequenced on an Illumina Novaseq platform to generate 150bp paired-end reads with a sequencing depth of 10 million reads per sample ...
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bioRxiv - Microbiology 2024Quote: ... Libraries for all samples were prepared using the Nextera™ DNA Flex Library Prep Kit (Illumina Inc., San Diego, CA, USA), and the subsequent shotgun sequencing was executed on a NovaSeq 6000 system (Illumina Inc. ...
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bioRxiv - Plant Biology 2024Quote: ... and only samples with Integrity Number of 7 or above were used for library construction by the TruSeq stranded mRNA protocol (Illumina, USA). Libraries were sequenced using an Illumina Novaseq6000 sequencer (Illumina ...
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bioRxiv - Immunology 2024Quote: ... One library consisting of a total of 4 samples were pooled and sequenced on the NextSeq 2000 (Illumina, San Diego, CA) using a P3 100 cycle kit (Illumina) ...
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bioRxiv - Physiology 2024Quote: ... and sequenced (75 nucleotides, single end) with a median depth of 66 million reads per sample by NextSeq 550 (Illumina, USA). Raw data were deposited to NCBI GEO ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to ensure sufficient RNA of high quality in each sample and prepared libraries using the Illumina TruSeq® mRNA Stranded Kit (Product#: RS-122-2101, Illumina, Inc. ...
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bioRxiv - Microbiology 2024Quote: Samples were prepared by generating a uniquely indexed library from each of the RNA samples isolated from male pupae using the Illumina Stranded Total RNA Prep with RiboZero Plus Microbiome kit (Illumina, USA) and custom D ...
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bioRxiv - Microbiology 2024Quote: ... A secondary indexing PCR was performed on normalised samples at 5 ng/μL using TG Nextera XT Index kit v2 Set B (#FC-131-2002, Illumina, USA). The products were purified using Agencourt AMPure XP magnetic beads and quantified using Qubit dsDNA HS ...
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bioRxiv - Neuroscience 2024Quote: ... 44M 150bp paired-end reads were sequenced per sample on an Illumina NovaSeq 6000 using the S4 Reagent Kit v1.5 (Illumina, Cat#20028313) by the Genome Technologies Core at The Jackson Laboratory.
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bioRxiv - Molecular Biology 2024Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina, RS-122-2001) but using Superscript III for reverse transcription instead of Superscript II (50 °C for 50’ incubation time) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA libraries of the radular tissues were prepared using TruSeq Stranded mRNA Sample Prep Kits (Illumina, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Samples were submitted for library generation and sequencing by the University of Chicago sequencing core using a NovaSeq 6000® (Illumina). Data was analyzed using Galaxy (https://usegalaxy.org/) ...
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bioRxiv - Genomics 2024Quote: ... The normalized libraries were sequenced as paired end 100bp reactions targeting 30 million reads/sample on the Illumina NovaSeq 6000 (Illumina, CA). The raw FASTQ files were processed through an in-house bioinformatics pipeline including adapter trimming by TrimGalore (v0.6.10 ...
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bioRxiv - Cell Biology 2024Quote: ... All samples were then pooled in equimolar ratios and sequenced using Illumina NextSeq 500/500 v2 75 cycle kit (Illumina 20024906). Amplifications were carried out with 1×8 cycles for sample index reads and 1×63 cycles for the sgRNA.
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bioRxiv - Developmental Biology 2024Quote: Sample libraries were prepared for Illumina sequencing by denaturing and diluting the libraries per manufacturer’s protocol (Illumina, San Diego, CA, USA). All samples were pooled into one sequencing pool with biased pooling based on variable cell numbers per sample ...
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bioRxiv - Genomics 2024Quote: ... Clustering of the index-coded samples was performed on a cBot Cluster Generation System via the TruSeq PE Cluster Kit v3-cBot-HS (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: PCR-free libraries were generated from 1 microgram genomic DNA using a Covaris R230 to shear the DNA and the TruSeq® DNA PCR-Free HT Sample Preparation Kit (Illumina) for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... 1 µg of total RNA per sample was used for RNA-seq library preparation using the Illumina TruSeq stranded mRNA kit (Illumina, 20020595). Paired-end sequencing was performed on a NovaSeq (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of total RNA was used with the TruSeq RNA Sample Prep Kit v.2 (Illumina, San Diego, CA, USA). The cDNA libraries were quantified by quantitative Real-Time PCR (qPCR) ...