Labshake search
Citations for Illumina :
3701 - 3750 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from similar pooled-samples (N = 3) using the ZYMO (Irvine, CA, USA) direct-zol miniprep kit (Cat. # R2050) and sequenced using NovaSeq (Illumina, San Diego, CA, USA) paired-end (150 bp ...
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bioRxiv - Genomics 2023Quote: ... Approximately one microgram of RNA from each tissue sample was utilized to prepare libraries using the TruSeq Stranded mRNA Library Preparation Kit with polyA selection (Illumina Inc., San Diego, USA). The libraries were paired-end sequenced in two separate batches ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were prepared from 1 µg of input DNA using a TruSeq DNA Sample Prep Kit according to the manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). The sheared DNA fragments underwent end-repair ...
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bioRxiv - Zoology 2024Quote: ... the samples and negative controls were pooled and subjected to sequencing on the NextSeq 2000 platform (2 × 100 cycles) (Illumina, San Diego, CA, USA).
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bioRxiv - Neuroscience 2024Quote: ... and libraries were constructed by using 600 ng of total RNA from each sample and utilizing a TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following the manufacturer’s suggested protocol ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 1 Dg of total RNA was used to prepare small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA) and single-end sequencing 50bp on an Illumina Hiseq 2500 (following the vendor’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: ... alpha/beta libraries were prepared on 100 ng of RNA from each sample using the SMARTer Mouse TCR α/β Profiling Kit from Takarabio® and sequenced using the MiSeq V2 2×150bp (Illumina®) and HiSeq2500 V2 2×150bp (Illumina® ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: cDNA were used in the library preparation using Ovation® Ultralow Library Systems (NuGEN) and samples were sequenced using an Illumina HiSeq 2000 sequencer (Illumina, San Diego, CA) in high output mode across 9 lanes of 50bppaired-end sequencing ...
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bioRxiv - Genetics 2024Quote: ... Bisulfite-converted DNA samples were randomly assigned to a chip well on the Infinium Human Methylation EPIC v2 BeadChip (Illumina, Inc., San Diego, CA) or in the Human Imprintome array BeadChip (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Between 3-5 µg of total RNA from each sample was used for mRNA enrichment with the RiboZero Bacterial rRNA Removal Kit (Illumina, San Diego, CA, USA), and the quality and quantity of enriched mRNA was assessed as described above for total RNA ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approachbioRxiv - Genetics 2022Quote: ... we used 400-ng of each DNA sample to construct indexed sequencing libraries (Illumina DNA Prep Tagmentation, 20018705; Illumina Nextera DNA CD Indexes, 20018708). Libraries were mixed by replicate into two 4-plexes ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... All samples showed a high RNA quality (RNQ>8) and were sequenced using an Illumina® HiSeq 3000 sequencer (Illumina, San Diego, CA, USA). Reads were mapped to the Sscrofa11.1 assembly of the Swine Genome Sequencing Consortium (SGSC ...
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bioRxiv - Genomics 2022Quote: ... 500 ng per sample of isolated DNA was bisulfite converted and hybridized with the Infinium Mouse Methylation BeadChip (Illumina Inc., San Diego, CA, USA), from 24 C57BL/6 mice (all female ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... for 16p11.2 del/+ mice were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Pooled libraries were sequenced on Illumina NovaSeq 6000 sequencers with 100-bp Paired-End chemistry (Illumina) ...
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bioRxiv - Genomics 2023Quote: Illumina sequencing reads (∼54 million paired end reads per sample) were de-multiplexed generating compressed FASTQ files by the on-board DRAGEN informatics pipeline (Illumina DRAGEN FASTQ Generation – 3.7.4) on the NextSeq 2000 ...
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bioRxiv - Genomics 2023Quote: Illumina sequencing reads (∼50 million paired end reads per sample) were de-multiplexed generating compressed FASTQ files by the on-board DRAGEN informatics pipeline (Illumina DRAGEN FASTQ Generation – 3.7.4) on the NextSeq 2000 ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing reads (∼6 million paired end reads per sample) were de-multiplexed generating compressed FASTQ files by the on-board DRAGEN informatics pipeline (Illumina DRAGEN FASTQ Generation – 3.7.4) on the NextSeq 2000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries with an insert size of 350 bp were constructed using a TruSeq Nano DNA HT Sample Preparation Kit (Illumina, San Diego, California, U.S.A.) following manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: The bacterial swab and lake samples were subjected to 16S rRNA gene amplicon sequencing on an Illumina MiSeq platform (Illumina Inc., San Diego, USA). The sequencing library was prepared according to a two-step PCR ...
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bioRxiv - Microbiology 2023Quote: ... The microbial community of each sample was analyzed by 16S rRNA gene amplicon sequencing of the V4-V5 hypervariable region (Illumina MiSeq platform) (Illumina Inc., San Diego, CA) using 515F and 806R universal primers at the MrDNA Laboratory (Molecular Research LP ...
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bioRxiv - Physiology 2024Quote: ... were used to construct individual Poly-A enriched cDNA libraries by Novogene (Beijing, China) using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacture’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of the extracted total RNA was used for library construction using the TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... followed by a nested PCR reaction (5 cycles for 70 sec at 72◦C) with individual sample-barcoded i5 and i7 Illumina TruSeq primers (New England Biolabs NEBNext Multiplex Oligos for Illumina, cat. # E7600S; Table S1B) 2 ...
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bioRxiv - Cell Biology 2024Quote: ... was performed at 25 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina, San Diego, CA, USA) using High output sequencing kit ...
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bioRxiv - Microbiology 2024Quote: Paired-end libraries (2×151 bp) were prepared for a subset of samples using Illumina TruSeq DNA library preparation kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina NextSeq500 platform ...
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bioRxiv - Neuroscience 2024Quote: Total RNA extracted from hippocampus tissue samples were used for library preparations and sequenced on an Illumina Hiseq 2500 platform (Illumina, San Diego, CA, USA). The biological significance of altered miRNA expression is closely related to their gene targets ...
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bioRxiv - Genomics 2024Quote: SARS-CoV-2 genome sequencing was performed on samples that were SARS-CoV-2 RNA-positive using the COVIDSeq Test (Illumina, San Diego, CA, USA). Libraries were sequenced on the Illumina NextSeq2000 instrument using 2×109 paired end reads ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced on the Illumina NovaSeq 6000 sequencer with the S1-200 reagent kit to generate approximately 60 million 100- base read pairs per sample (Illumina, Inc., San Diego, CA). Read adapters were detected using BBMerge (v37.02 ...
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bioRxiv - Physiology 2024Quote: ... 500 ng of RNA per sample were used to prepare sequencing libraries using TruSeq Stranded mRNA Library Prep Kit from Illumina (catalog #20020595 and 20022371). Libraries were quantified using the Qubit 4 fluorometer and dsDNA BR DNA assay (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Illumina RNAseq libraries were prepared from 2 μg total RNA using the Illumina TruSeq™ RNA Sample Prep Kit v2 according to the manufacturer’s instructions (Illumina Inc. San Diego, CA). All RNAseq libraries (150-750 bp inserts ...
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bioRxiv - Cell Biology 2024Quote: ... Clustering of the index-coded samples was performed using an acBot Cluster Generation System using TruSeq PE Cluster Kitv3-cBot-HS (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA obtained from each sample was subjected to a sequencing library construction using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc., San Diego, CA) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... RNA samples with a RIN value ≥ 8 were used to prepare sequencing libraries with the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocol.
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bioRxiv - Genetics 2024Quote: cDNA libraries for RNA-Seq were constructed from the extracted RNA samples using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) following the procedures outlined in the Reference Guide ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were centrifuged at 500 g for 10 min and immediately resuspended in 25 µl of buffer containing 2.5 µl of Tn5 transposase (Illumina, Inc., San Diego, CA cat # 15027865) for a 30 min incubation at 37oC ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 600 nL of tagmentation reaction mix containing Tagmentation DNA buffer (TD) and Amplicon Tagment Mix (ATM) at 2:1 v/v ratio (Nextera kit, Illumina, cat. no. FC-131-1096) into each well and performed tagmentation at 55° C for 5 min followed by hold at 4° C in a PCR thermocycler ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a total of 4 μg of total RNA was used to construct paired-end libraries using the TruSeq RNA Sample Preparation Kit v3 (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... This work benefited from the facilities and expertise of the I2BC High-throughput Sequencing Platform (Gif-sur-Yvette, Université Paris-Saclay, France) for oriented library preparation (Illumina Truseq RNA Sample Preparation Kit) and sequencing (Paired-end 75 bp ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were constructed from 300 ng of total RNA from sample provided by our laboratory using the TruSeq® Stranded Total RNA Library Prep (Illumina, Inc. San Diego, CA) following the recommended protocol ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Genomics 2020Quote: ... Post-capture DNA libraries were combined into 10 pools of approximately 96 samples each and sequenced on three lanes on an Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) to generate approximately 0.5 million 2x150-bp paired-end reads per sample ...
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bioRxiv - Genomics 2020Quote: ... Further sample QC was performed by Illumina to ensure that the concentration of the DNA was > 30 ng/μl and that every sample generated high quality genotyping results (Illumina Infinium Human Core Exome microarray). Samples with a repeated array genotyping call rate < 0.99 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We prepared a genomic DNA library of these 343 DNA samples using a modified version of the Nextera DNA library prep kit (∼ 350bp insert size, Illumina Inc., San Diego, CA, USA) meant to conserve reagents ...