Labshake search
Citations for Illumina :
3501 - 3550 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The beads were resuspended in 50 μl of the following PCR master mix with indexed primers from the Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011): Phusion HF 2X (NEB ...
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bioRxiv - Genomics 2020Quote: 54 samples were sequenced on two Illumina HiSeq 4000 lanes using 150 bp paired-end chemistry with 300 cycles (Illumina, San Diego, CA). All remaining samples were sequenced on three Illumina NovaSeq lanes using S4 150 bp paired-end chemistry with 300 cycles ...
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bioRxiv - Genomics 2020Quote: ... The 300 ng gDNA of all four samples were used to prepare paired-end libraries with the Nextera ™ DNA Flex Library Preparation kit with an average insert size of 600 bp for all four samples according to the manufacturer’s instructions (Illumina Inc., San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Six hundreds pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Immunology 2021Quote: ... greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... RNA library from homecage and fear conditioned mice were prepared at the PGFI sequencing core at the University of Pennsylvania using the TruSeq sample preparation kit (Illumina San Diego, CA) according to the manufacturer’s instructions with polyA selection ...
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bioRxiv - Neuroscience 2020Quote: ... Most PNC subjects were genotyped from blood samples on the 550HH and 610-Quad SNP arrays from Illumina (Illumina Inc., San Diego, CA). Analyses were performed using PLINK 1.9 (Chang et al. ...
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bioRxiv - Neuroscience 2020Quote: IMAGEN subjects were genotyped from blood samples on 610-Quad SNP and 660-Quad SNP arrays from Illumina (Illumina Inc., San Diego, CA). Most PNC subjects were genotyped from blood samples on the 550HH and 610-Quad SNP arrays from Illumina (Illumina Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 0.4 - 1 μg of total RNA were used to prepare small RNA cDNA libraries utilizing the specified protocol for the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, USA). Single-read sequencing of 50 bp was performed on an Illumina Hiseq 2500 at LC Sciences LLC (Houston ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation for RNA-Seq was performed using the TruSeq™ RNA Sample Prep Kit v2 (Illumina, Cat. N°RS-122-2002, USA) starting from 50 ng of total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... 50bp RNA-seq of IP fractions and total homogenate from 5 replicates per cell type (20 samples total) was performed on a single lane of a HiSeq 3000 (Illumina, San Diego, California), yielding ∼350M reads ...
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bioRxiv - Plant Biology 2021Quote: ... A paired-end library and four mate libraries were constructed using a TruSeq DNA PCR-Free Library Prep Kit and a Nextera Mate Pair Sample Preparation Kit (Illumina, San Diego, CA), respectively ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were prepared from single frond samples using 1μg starting RNA using the TruSeq RNA library prep kit V2 (Illumina Systems, San Diego, USA) and quantified by KAPA library quantification (Roche ...
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bioRxiv - Microbiology 2021Quote: Paired-end DNA and cDNA libraries (2 × 150 bp) were constructed using TruSeqTM DNA Sample prep kit (Illumina Inc., San Diego, CA, USA) and TruSeqTM RNA Sample prep kit (Illumina Inc. ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was sent to Beijing Genomics Institute for library prep with the TruSeq small RNA sample preparation kit (Illumina, San Diego, California) and sequencing by 50 bp single end sequencing with the BGISEQ-500 platform ...
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bioRxiv - Microbiology 2021Quote: ... All 24 samples were multiplexed together into a single High Output 2×75bp run on a NextSeq 500 instrument (Illumina, San Diego, CA). Raw reads were mapped to the C ...
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bioRxiv - Microbiology 2020Quote: ... For all 2018 samples sequencing was performed using an Illumina MiSeq and the MiSeq Reagent Kit v2 (Illumina, Inc., San Diego, CA, USA) for 2 sets of 250 cycles ...
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bioRxiv - Microbiology 2020Quote: ... One µg total RNA from each sample was used to generate a paired-end RNA-seq library by polyA mRNA workflow using TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA). Each library was subjected to sequence in Illumina Novaseq 6000 (2 × 150bp read length ...
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bioRxiv - Cancer Biology 2020Quote: ... University of Pittsburgh sample libraries were manually prepared via standard protocols using the Illumina TruSeq RNA Exome Library Prep Kit (Illumina, San Diego, CA) and sequenced on an Illumina NextSeq sequencing system using NextSeq 500 Mid- and High-Output 150 cycle kits (75 bp ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were converted to RNA-seq libraries with the TruSeq Stranded Total RNA Library Preparation Gold kit (Illumina, San Diego, CA, USA). This kit was reported to reliably and reproducibly generate libraries from 1-2 ng input RNA (Schuierer et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The mRNA was subsequently fragmented and used for cDNA synthesis and library preparation using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA libraries were constructed and amplified using random primers according to the manufacturer’s instructions (Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing). For sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Duke University Center for Genomic and Computational Biology) for sequencing on an Illumina MiSeq Instrument resulting in 20,000 paired-end 250bp reads per sample (Illumina, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina, San Diego, CA). Data analysis was performed as described before (56).
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bioRxiv - Genomics 2022Quote: ... paired-end library was prepared using 100 ng of DNA with the Nextera DNA sample preparation kit as per the manufacturer’s instructions (Illumina, Inc., San Diego, USA). This was followed by sequencing on Illumina NextSeq 500 and HiSeq X 10 platforms with a paired-end run of 2×150 bp ...
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bioRxiv - Cell Biology 2022Quote: ... using poly-T oligos attached to magnetic beads and converted to sequence-ready libraries using the TruSeq stranded mRNA sample prep protocol (cat. #: RS-122-2101, Illumina, San Diego, CA). The final libraries were validated on the bioanalyzer DNA chips ...
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bioRxiv - Developmental Biology 2020Quote: ... 1ug of total RNA was then used to generate sequencing libraries using the TruSeq RNA Sample prep kit v2 (Illumina RS-122-2001), as per recommended instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were pooled at a concentration of 5nM and run on an Illumina HI-SEQ 2500 sequencer (Illumina, Inc. San Diego, CA) to obtain paired-end reads of 75 bases (PE75) ...
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bioRxiv - Neuroscience 2021Quote: ... 300 pg of cDNA from each sample was used as input for library preparation with the Nextera XT DNA Library Prep Kit (Illumina FC-131-1096). Fragmentation and adaptors insertion were performed by tagmentation ...
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bioRxiv - Microbiology 2020Quote: ... Individual libraries were constructed from some of the strain DNA preparations described above using an Illumina Nextera XT DNA sample preparation kits with appropriate indices tags according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). The libraries were pooled together and run on an Illumina MiSeq DNA sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: The cDNA synthesis and library construction were done using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation for RNA-Seq was performed using the TruSeq™ RNA Sample Prep Kit v2 (Illumina, Cat. N°RS-122-2002, U.S.A.) starting from 50 ng of total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... where the DNA was size-selected using a yBluePippin (Sage Science) before the sample was sequenced on an Illumina MySeq (Illumina pipeline software v2.18) with paired-end 250 base read length.
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bioRxiv - Immunology 2022Quote: ... The amplicons were quantified using KAPA Library Quantitation kit (KAPA, Cape Town, South Africa) and an equimolar amount of each sample was sequenced on the MiSeq System (Illumina, San Diego, CA) with 2×150 paired-end run parameters ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-Seq libraries were generated from 300 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and IDT for Illumina - TruSeq RNA UD Indexes (96 Indexes, 96 Samples) (Illumina, San Diego, USA), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Post-ligation cleanup proceeded according to Illumina’s instructions with 110 µL Sample Purification Mix from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina catalog # FC-151-1002). After purification ...
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bioRxiv - Genetics 2022Quote: ... Individual barcoded libraries were randomly multiplexed into groups of four samples using equimolar quantities and sequenced on a MiSeq using a v3 600 reagent kit (Illumina, San Diego, CA) to generate paired-end 250 bp reads ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Two independent paired-end libraries were constructed per population from these DNA pools using the Genomic DNA Sample Preparation Kit (Illumina, San Diego, CA) (technical replicates) ...
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bioRxiv - Cancer Biology 2020Quote: ... and one sample of each clonal population) were multiplexed into one pool and sequenced on Illumina HiSeq2000 instrument (Illumina Inc., San Diego, CA). Adapter sequence was trimmed using Trimmomatic-0.30 ...
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bioRxiv - Systems Biology 2020Quote: ... Total-RNA stranded transcriptome libraries were prepared using the TruSeq Stranded Total RNA LT Sample Prep Kit starting from the fragmentation step in the manufacturers protocol (Illumina, RS-122-2201). Paired-end sequencing (2×125-bp ...
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bioRxiv - Genomics 2020Quote: ... Libraries were indexed and pooled into 12 sample sets and sequenced as 101 bp paired-end reads using Illumina HiSeq2500 (Illumina, San Diego, CA). Raw data have been submitted to GEO (http://ncbi.nlm.nih.gov/geo ...
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bioRxiv - Genomics 2021Quote: ... 200ng of input RNA was subjected rRNA depletion according to the Ribo-Zero Gold rRNA depletion protocol (Illumina, 96 samples, Cat no. 20020599). Libraries were prepared from the depleted RNA using the Illumina TruSeq Stranded Total RNA Library Prep Kit (Cat no ...
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bioRxiv - Neuroscience 2021Quote: ... 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... Six hundred pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Genetics 2020Quote: ... Non-stranded cDNA libraries were prepared with a TruSeq RNA sample preparation kit v2 and sequenced on a HiSeq 2000 (Illumina, San Diego, CA) using SBS v3-HS reagents for paired-end sequencing (2 x 100bp) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries from 1000-4000 cells (depending on the sample) were sequenced in paired end mode on a HiSeq 4000 instrument (Illumina, San Diego, CA) at an average depth of 174,446 reads per cell (for V2/V3) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were generated with 1 μg of RNA as input using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) with poly(A)-enrichment according to the TruSeq protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina short reads DNA libraries were generated from the pooled amplicons using Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer specifications ...
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bioRxiv - Neuroscience 2021Quote: 1 uL of diluted cDNA per well was used for library preparation using the Nextera XT DNA Sample Prep Kit (Illumina, #FC-131-1096). Each C1 IFC was pooled into one library ...