Labshake search
Citations for Illumina :
3651 - 3700 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Illumina Paired-End libraries were constructed for three DNA samples using the Illumina Tru-seq DNA Library Prep Kit (Illumina, San Diego CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... sequencing was performed at a targeted depth of 40 million reads per sample using the Illumina NextSeq 500 platform (Illumina Inc, San Diego, CA). mRNA reads (4 regions ...
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was sent to the University of Illinois Keck Center for Fluidigm sample preparation and paired-end (2 × 250 nt) sequencing on an Illumina NovaSeq (Illumina, San Diego, CA, USA). The 16S rRNA gene fragment was amplified using the primers V3_F357_N ...
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bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA and ligated to sequencing adaptors containing indexes using the Illumina TruSeq stranded mRNA sample preparation kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and poly-A sequencing libraries (HE2.1 and TIVE cells) were generated using the TruSeq RNA Sample Prep Kit (Illumina, catalog no. FC-122-1001).
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bioRxiv - Molecular Biology 2022Quote: 4µg of total pooled RNA of all parts was taken for paired-end sequencing and prepared cDNA libraries by the use of Illumina TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, California, USA) as per it’s described protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The library was made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit - set B (Illumina, Cat. No. RS-122-2102). The resulting short fragment library was checked for quality and quantity using the LabChip GX (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing was done using the Illumina NextSeq500 to obtain >20 million 75bp single-end or 37bp paired-end reads per sample or at Genewiz (HiSeq, 150bp, paired end) (Illumina, South Plainfield, NJ, USA).
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bioRxiv - Neuroscience 2022Quote: ... and libraries were constructed by using 600 ng of total RNA from each sample and utilizing a TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following the manufacturer’s suggested protocol ...
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bioRxiv - Immunology 2022Quote: ... Paired-end (2×75) sequencing was performed to an average of 40 million reads per sample on a HiSeq 3000 (Illumina, San Diego, CA, USA).
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bioRxiv - Genetics 2022Quote: 1184 recombinant inbred lines of the S6 generation of the Global MAGIC population were genotyped from DNA extracted from leaf tissue using Genotyping-by-Sequencing (GBS, with the enzyme ApeKI) across 14 lanes of 96-sample multiplexed sequencing (100 bp single-end Illumina, Phred+33 fastq format) as reported in Zaw et al ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina; San Diego, CA, USA), and the library preparations were then sequenced on an Illumina NovaSeq 6000 platform (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... An Illumina paired-end DNA library with an insert size of ∼300 bp was constructed using the Genomic DNA Sample Prep kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... we sequenced a subset of 12 samples (six Salix and six Xanthium from both extraction methods) with the Illumina technology (Illumina Inc., San Diego, USA). Libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (for old herbarium specimens ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Libraries were prepared using the Illumina TruSeq stranded mRNA sample preparation kit according to the manufacturer’s protocol (Illumina, Inc., Hayward, CA; RS-122-2103). Approximately 30 million 2 x 50 bp paired-end reads were collected per sample using the Illumina HiSeq2000 sequencing platform ...
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bioRxiv - Microbiology 2022Quote: A cDNA library was prepared from RNA extracted from bat sarbecoviral isolates using the TruSeq Stranded Total RNA LT Sample Prep Kit Gold (Illumina, San Diego, CA, USA) for Rc-mk2 and Rc-kw8 strains or the MGIEasy RNA Directional Library Prep kit (MGI ...
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bioRxiv - Genetics 2020Quote: ... and ligated to index-containing sequence adaptors using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina, San Diego, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The trimmed DNA fragment sequences were then used to prepare a shotgun paired-end library with an average insert size of 350 bp using a TruSeq DNA Sample Prep kit (Illumina, San Diego, CA, USA). The library was sequenced on an Illumina MiSeq platform (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... on Thermo Fisher Scientific’s Quantstudio 5 before multiplex pooling (13-16 samples per lane in equal representation) and sequencing on the NextSeq500 platform (Illumina, San Diego, California, USA) paired-end 2×150 bp High Output.
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bioRxiv - Genetics 2021Quote: ... The thirty-seven sequencing libraries were created by reverse-transcription from 2 µg of RNA from each sample using the TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA). Index adapters were added to identify sequences for each sample in the final data ...
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bioRxiv - Immunology 2020Quote: ... All 11 samples were subjected to an indexed paired-end sequencing run of 2×100bp on an Illumina Novaseq 6000 system (Illumina, San Diego, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The clustering of the index-coded samples was performed on an acBot Cluster Generation System using TruSeq PE Cluster Kitv3-cBot-HS (Illumina, San Diego, CA, USA). The library preparations were then sequenced on an Illumina Hiseq platform ...
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bioRxiv - Genomics 2020Quote: ... RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... BS-converted DNA (100 ng) of each sample was used for preparing the BS-seq library via the TruSeq DNA Methylation Kit (Illumina, EGMK91324, San Diego, CA). The quality of the libraries was checked using Qubit assays (Q32850 ...
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bioRxiv - Microbiology 2021Quote: The sequencing libraries were constructed and indexed with Nextera DNA Sample Preparation kit and Index kit as recommended by the manufacturer (Illumina, San Diego, CA, USA). Paired-end sequencing was performed using a HiSeq 4000 instrument (2 x 150 bp ...
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bioRxiv - Microbiology 2020Quote: ... Five DNA extractions were performed per soil sample and pooled for metagenomic library preparation with the TrueSeq DNA kit (Illumina Inc., San Diego, CA). Sequencing was performed on the Illumina HiSeq platform to generate 6.4 billion of paired-end reads of 150bp.
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bioRxiv - Immunology 2021Quote: ... All 72 samples were sequenced on six separate flow cells (all samples were loaded onto all flow cells) on an Illumina Hi-Seq 2500 (Illumina, Inc., San Diego, California) with 125 bp paired-end reads following manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: Library preparation for sequencing was performed using 200 ngs of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including Adapters in average size ...
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bioRxiv - Genomics 2021Quote: ... We performed the cfMeDIP-seq experiment as previously described.2 Samples underwent sequencing (Princess Margaret Genomics Centre, Toronto, ON, Canada) on a NovaSeq 6000 (Illumina, San Diego, CA, USA), paired-end 2×100 bp ...
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bioRxiv - Genomics 2021Quote: ... Samples were pooled into three final libraries and sequenced by Novogene (Sacramento, CA, USA) on an Illumina HiSeq platform (Illumina, San Diego, CA, USA) producing 150 bp paired-end reads.
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bioRxiv - Genetics 2020Quote: ... The two resulting PCR products were mixed in equimolar amounts and each sample was barcoded according to the Nextera XT library preparation protocol (Illumina, San Diego, CA, USA). Sequencing was performed on the Illumina MiSeq platform (DNA Core Facility ...
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approachbioRxiv - Genetics 2022Quote: ... we used 400-ng of each DNA sample to construct indexed sequencing libraries (Illumina DNA Prep Tagmentation, 20018705; Illumina Nextera DNA CD Indexes, 20018708). Libraries were mixed by replicate into two 4-plexes ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... All samples showed a high RNA quality (RNQ>8) and were sequenced using an Illumina® HiSeq 3000 sequencer (Illumina, San Diego, CA, USA). Reads were mapped to the Sscrofa11.1 assembly of the Swine Genome Sequencing Consortium (SGSC ...
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bioRxiv - Genomics 2022Quote: ... 500 ng per sample of isolated DNA was bisulfite converted and hybridized with the Infinium Mouse Methylation BeadChip (Illumina Inc., San Diego, CA, USA), from 24 C57BL/6 mice (all female ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... for 16p11.2 del/+ mice were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Pooled libraries were sequenced on Illumina NovaSeq 6000 sequencers with 100-bp Paired-End chemistry (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 1 ug of total RNA was then used to prepare small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... multiplexed at 72-96 samples per run in paired-end mode with 325 × 275 cycles using the MiSeq Reagent v3 600-cycle kit (Illumina Inc., San Diego, CA).
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bioRxiv - Microbiology 2022Quote: Shotgun metagenomic sequencing libraries were prepared using either the Illumina Nextera XT Library Preparation Kit (39 samples) or the Illumina DNA Prep library Kit (Illumina Inc., San Diego, CA) (13 samples) ...
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bioRxiv - Plant Biology 2024Quote: ... More than 10 µg of RNA per sample was sent to Novogene (Cambridge, UK) for library preparation and massive sequencing on an Illumina Novaseq 6000 (Illumina, San Diego, CA, USA).
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bioRxiv - Genetics 2024Quote: ... Bisulfite-converted DNA samples were randomly assigned to a chip well on the Infinium Human Methylation EPIC v2 BeadChip (Illumina, Inc., San Diego, CA) or in the Human Imprintome array BeadChip (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Between 3-5 µg of total RNA from each sample was used for mRNA enrichment with the RiboZero Bacterial rRNA Removal Kit (Illumina, San Diego, CA, USA), and the quality and quantity of enriched mRNA was assessed as described above for total RNA ...
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bioRxiv - Zoology 2024Quote: ... the samples and negative controls were pooled and subjected to sequencing on the NextSeq 2000 platform (2 × 100 cycles) (Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...