Labshake search
Citations for Illumina :
3601 - 3650 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Sequencing libraries of individual DNA samples were prepared using the Illumina TruSeq DNA PCR-free LP kit (Illumina, San Diego, California, United States) in combination with the IDT for Illumina TruSeq DNA UD indexes kit (Illumina) ...
-
bioRxiv - Genomics 2023Quote: ... The libraries were sequenced at a target depth of 20,000 paired-end 150 bp reads per sample using a MiSeq machine (Illumina, San Diego, CA, USA). Reads were filtered by length <150 bp and using a maximum expected error threshold of 1.0 ...
-
bioRxiv - Microbiology 2023Quote: ... Prepared libraries were sequenced to an expected sequencing depth of 100,000 paired-end reads per sample on an Illumina MiSeq instrument (Illumina Inc, San Diego, CA) using a 600 (2×300 base pair ...
-
bioRxiv - Microbiology 2023Quote: ... Separate libraries for the two experimental conditions belonging to three independent experiments were prepared with the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... Libraries were pooled in groups of twelve and sequenced (twelve samples per lane) on an Illumina HiSeq 2500 ultra high-throughput DNA sequencing platform (Illumina, San Diego, CA) at the DNA Core facility at the University of Missouri ...
-
bioRxiv - Cancer Biology 2023Quote: RNA libraries were prepared from 550 ng of total RNA from tumour samples using the TruSeq Stranded Total RNA HT kit with Ribo-Zero Gold (Illumina, San Diego, USA) as per manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Total RNA-Seq libraries were generated from 150-300 ng of total RNA using TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA), according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200ng mRNA per sample was used for RNA-seq library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina RS-122-2001) according to manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: The input samples were submitted to the Washington University Genome Technology Access Center to obtain and sequence the cDNA libaraies (Illumina NovaSeq X Plus) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: Whole-genome sequencing was performed on each sample using Illumina Technology (MiSeq and HiSeq 2500) and Nextera XT library preparation kits as instructed by the manufacturer (Illumina, San Diego, CA). Fastq files (raw sequencing data ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA samples with a RIN value ranging from 6.2 to 8.6 were subjected to sequencing library preparation using TruSeq Stranded mRNA Low Throughput (LT) Sample Preparation Kit (Illumina, San Diego, CA, USA). The prepared library was sequenced using NovaSeq6000 sequencer (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: Approximately 1 μg of total RNA extracted from mouse livers (n=3 or 4 for each group) was used for RNA-seq library preparation following the Illumina TruSeq stranded mRNA sample preparation guide (Illumina, San Diego, CA). The methods for RNA sequencing and analysis were described in Supplementary information.
-
bioRxiv - Cell Biology 2024Quote: ... library was generated for 10 plasma exosome samples (n=5, young and old individuals each) using a propriety Illumina Truseq™ Small RNA Preparation kit (Illumina, # RS-930), according to the manufacturers’ guide ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... These libraries (16 otolith samples from 1996 from pilot and 40 finclip samples from 2014 and 2019) were sequenced on Illumina 6000 S4 Flowcell (Illumina, San Diego, USA) by CCGA Kiel ...
-
bioRxiv - Genomics 2024Quote: ... then reverse transcribed to cDNA and prepared for next-generation sequencing according to the manufacturer’s protocol (Illumina TruSeq RNA Sample Preparation v2 Guide). The cDNA libraries were validated on the Agilent 2100 Bioanalyzer using DNA-1000 chip ...
-
bioRxiv - Molecular Biology 2024Quote: ... Individual purified libraries were pooled into two six-sample equimolar pools containing the indexes 3-8 (NEBNext multiplex oligos for Illumina, set 1, E7335) and deep-sequenced at BGI on a HiSeq4000 (paired-end 100-bp reads).
-
bioRxiv - Pathology 2024Quote: ... 200 ng RNA was used to prepare sequencing libraries using the TruSeq Stranded Total RNA with RiboZero following the low sample protocol as per manufacturer’s recommendations (Illumina, San Diego, CA, USA), as previously described43 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 250 ng of genomic DNA were genotyped for each sample using the Neuro Global Diversity Array-8 v.1 LCG with Neuro Booster content (Illumina, 20031815 and 20042459) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA sample library and RNA-sequencing were performed by the Genomics Core Laboratory at Weill Cornell Medicine with Illumina TruSeq Stranded mRNA Sample Library Preparation kit (Illumina, San Diego, CA), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Single-indexed and multiplexed samples were run on an Illumina Next Seq 500 sequencer using a NextSeq 500 v2 kit (FC-404–2005; Illumina, San Diego, CA) for paired-end sequencing ...
-
bioRxiv - Genetics 2024Quote: ... The TrueMethyl converted DNA samples were then eluted in 10 µL and then processed through the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA) protocol ...
-
bioRxiv - Plant Biology 2024Quote: RNA from selected samples was used to prepare RNA-Seq libraries using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA). After their fragment size profiles were verified on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Plant Biology 2024Quote: ... The number of individual samples per replicate was designed to also fit one sequencing run on the NovaSeq 6000 S4 flow cell machine (Illumina, San Diego, USA), described later.
-
bioRxiv - Microbiology 2024Quote: All bacterial strains to be sequenced were grown overnight in LB in 15 ml falcon tubes and DNA samples were obtained using the Epicentre MasterPure Complete DNA and RNA Purification Kit (Illumina, San Diego, USA). DNA libraries were prepared using the Illumina DNA Prep kit according to the manufacturer’s guidelines ...
-
bioRxiv - Genomics 2024Quote: ... Libraries were constructed following the instructions of the Illumina ChIP-seq Sample Prep Kit (Cat# IP-102-1001, Illumina, San Diego, CA, USA) and sequenced to generate 150-bp paired-end reads on the Illumina NovaSeq-6000 platform.
-
bioRxiv - Genomics 2024Quote: ... mRNA enrichment and preparation of barcoded cDNA libraries were done using the TruSeq RNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA). F ...
-
bioRxiv - Developmental Biology 2024Quote: Total RNA samples were prepared for mRNA-Sequencing using the Illumina Stranded mRNA Ligation Sample Preparation kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). Libraries were validated for lengths ...
-
bioRxiv - Developmental Biology 2024Quote: ... 500 ng of total RNA from each sample was used for the RNA-Seq library preparation using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) following the manufacturer’s instructions[7] ...
-
bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... sequencing primers diluted in HT1 buffer to a final concentration of 500 nM (Fluidigm, see Access Array System for Illumina Sequencing Systems user guide) on a MiSeq or HiSeq 2000 next generation sequencing instrument according to the manufacturer’s protocols (Illumina ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2ng of DNA was prepared in 30ul of buffer and used for library construction using the Nextera DNA Flex Library Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s guidelines ...
-
bioRxiv - Developmental Biology 2022Quote: ... Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
-
bioRxiv - Immunology 2024Quote: ... transposition reaction was done with 350 rpm mixing and using the transposase from the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, San Diego, CA, USA). Libraries were quantified using KAPA Library Quantification kit (Roche ...
-
bioRxiv - Immunology 2023Quote: ... 3mM MgCl2) on ice and resuspended in 10μl of transposition mix (1X TD Buffer from Illumina, Transposase from Illumina, 0,01% digitonin, 0,1% Tween-20). Tagmentation was performed on a thermomixer at 1000rpm 37°C during 30min ...
-
bioRxiv - Immunology 2024Quote: ... Transposition was performed directly on nuclei using 25µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina and Digitonin #G9441 from Promega). After pelleting the nuclei by centrifuging at 500g for 10 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Transposition was performed directly on nuclei using 25 μl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
-
bioRxiv - Cell Biology 2020Quote: ... Stranded mRNA-seq libraries were prepared at EMBL Genecore facility from 200ng of total RNA using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...
-
bioRxiv - Genetics 2020Quote: Blood samples of forty eight female GRF goats were collected and animals were genotyped with the Illumina GoatSNP50 BeadChip (Illumina Inc., San Diego, CA) containing 53,347 Single Nucleotide Polymorpishms (SNPs ...
-
bioRxiv - Molecular Biology 2021Quote: ... newly synthesized RNA using TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA, Cat# RS-122-2301) according to the Illumina protocol with the following modifications ...
-
bioRxiv - Microbiology 2020Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
-
bioRxiv - Genomics 2020Quote: Total RNA was extracted from Epstein–Barr-virus-transformed lymphoblastoid cell lines (LCLs) by following the Illumina TruSeq v2 RNA Sample Preparation protocol (Illumina, Inc., San Diego, CA) by the Department of Genetic Medicine and Development at the University of Geneva ...
-
bioRxiv - Genomics 2020Quote: RNA libraries from all samples were prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... was prepared from total RNA from a testicular tissue sample of one BSW bull homozygous for the mutant T-allele at Chr6:58373887 using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The library was sequenced using an Illumina NovaSeq6000 instrument ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... About 3-Gb paired-end raw reads with a length of 100bp were generated for each sample using Illumina HiSeq 4000 platforms (Illumina, San Diego, CA, USA) at BGI-Tech (BGI ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 2 x 150 NextSeq500 Shotgun Libraries were prepared from each sample using an Illumina TruSeq® Nano DNA Library Prep Kit (Illumina Inc, CA, USA). The quality of each library was checked using the Agilent 4200 Tape Station (Agilent Technologies ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... These samples were sequenced (2 × 150 or paired end 150 nt read length) on the Novaseq 6000 platform (Illumina, Inc, San Diego, CA, USA) by the Roy J ...
-
bioRxiv - Genomics 2020Quote: ... Paired-end RNA libraries (2 × 150 bp) were prepared using total RNA purified from testis tissue using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The libraries were sequenced at an Illumina NovaSeq6000 instrument yielding 49,198,010 and 70,451,148 reads ...
-
bioRxiv - Immunology 2021Quote: ... libraries were prepared using the NEXTflex®Rapid Directional RNA-Seq Kit (Bio Scientific) and samples were sequenced 75 base pair single-end on Illumina NextSeq500 (Illumina, Utrecht DNA Sequencing Facility) and processed as described above ...
-
bioRxiv - Zoology 2021Quote: ... Meta-transcriptome library preparation of each pool was carried out using the TruSeq™ Stranded Total RNA Sample Preparation Kit from Illumina (San Diego, CA) after removal of host ribosomal RNA using Illumina Ribo-Zero™ rRNA Removal Kits (San Diego ...