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Citations for Illumina :
2701 - 2750 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... ATAC-seq libraries were prepared from 50,000 cells per replicate using Illumina Tagment DNA Enzyme and Buffer Kit (Illumina 20034197) according to the Omi-ATAC-seq protocol (Corces et al. ...
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bioRxiv - Immunology 2024Quote: ... After centrifugation at 500ξ g for 5min at 4°C, the cell pellets were resuspended in tagmentation-mix (containing Tagment DNA buffer, Tagment DNA Enzyme (Illumina), Digitonin (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl amplified cDNA was tagmented in 1x Tagmentation Buffer using 0.8 μl bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were resuspended in a transposition reaction mix prepared using the Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, 20034197) and incubated at 37 °C for 30 minutes on thermomixer at 1,000 rpm to allow for transposition-based DNA fragmentation and adapter ligation ...
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bioRxiv - Genomics 2023Quote: Pelleted nuclei were gently resuspended in a 50 μL transposition reaction mix composed of 25 μL 2× TD Buffer (Illumina), 2.5 μL Tn5 Transposes (Illumina ...
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bioRxiv - Genomics 2023Quote: ... We used 15 mins incubation on ice in the nuclei preparation step and the Tn5 reaction was performed in 50 μl of custom transposition buffer (10 mM Tris pH 8, 5 mM MgCl2 and 10% dimethylformamide) with 2.5 μl Tn5 transposase (Illumina, 20034197) at 37°C while mixing at 1000 rpm for 30 mins ...
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bioRxiv - Microbiology 2023Quote: ... then diluted to 150 pM in elution buffer supplied in the library prep kit and loaded to the iSeq100 flow cell for sequencing on iSeq100 (Illumina). After the NGS run ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated by directly lysing the cells with RLT buffer and using RNeasy Mini kits and used to prepare TruSeq Stranded mRNA (Illumina) sequencing libraries ...
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bioRxiv - Genomics 2023Quote: ... The sequencing libraries were generated on the streptavidin C1 beads using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, 20034198) and run on the Illumina Novaseq S4 flowcell.
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... 0.1% Tween-20 in PBS) and added 2.5µl of TDE1 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). We incubated the transposition reaction at 37°C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase [Illumina] ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... Second tagmentation: 35 nl tagmentation mixture (160 μl recipe: 148-152 μl 2 × TD buffer, 12-8 μl TDE1, Illumina) was dispensed into the selected nanowells of the chip ...
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bioRxiv - Developmental Biology 2024Quote: ... The pellet was then resuspended in 50µl of transposition mixture (1X Illumina Tagment DNA Buffer and 100nM Illumina TDE1 Tagment DNA Enzyme transposase (Illumina; 20034197), 0.33X DPBS ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing adapters were added to the bead-bound DNA by tagmentation with the Tagment DNA Enzyme and Buffer Kit (Illumina). After washes ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL amplified cDNA was tagmented in 1X Tagmentation Buffer using 0.8 µL bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were pooled and sequenced with 75bp single-end sequencing to a depth of 20 × 106 reads per sample on a NextSeq500 (Illumina). Raw sequencing reads were demultiplexed using bcl2fastq (v2.17.1.14 ...
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bioRxiv - Biochemistry 2020Quote: ... Clustering of the index-coded samples was performed on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Totally 3 μg of total RNA was ligated with 5′- and a 3′-adaptors sequentially with TruSeqTM Small RNA (or Stranded Total RNA in the case of cancer cells) Sample Prep Kit (Illumina). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were constructed from individual mouse samples and RNA-seq libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina platform ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-Seq libraries were generated from 1 μg of total RNA from duplicated samples per condition using the TruSeq LT RNA Library Preparation Kit v2 (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the RNA was used as input for the Illumina TruSeq® Stranded Total RNA HT/LT Sample Prep Kit (Illumina). Libraries were pooled and sequenced on an Illumina Hiseq 3000 PE150 platform ...
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bioRxiv - Cell Biology 2020Quote: ... The fragmentated mRNA samples were subjected to cDNA synthesis and library generation using the TruSeq mRNA library preparation kit (Illumina). Sequencing was performed with a NextSeq sequencer (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... and library enrichment were performed as described in the Low Throughput protocol of the TruSeq RNA Sample Prep Guide (Illumina). RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... and amplified before pooling samples for paired-end sequencing using a NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024906) and NextSeq 500 system (Illumina).
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bioRxiv - Immunology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All samples were pooled at an equimolar concentration for paired end 2 × 300 bp sequencing via the MiSeq system (Illumina) using the MiSeq Reagent Kit v3 (600 cycles) ...
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bioRxiv - Genetics 2021Quote: ... The scRNAseq samples were then sequenced on 1/7th of a single S4 lane on a NovaSeq 6000 platform (Illumina). For the ScISOr-Seq library ...
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bioRxiv - Genomics 2020Quote: ... Poly-A-enriched RNA was used to prepare libraries with TruSeq RNA Sample Preparation kit v2 y v4 (ref. RS-122-2001/2, Illumina) according to instructions from manufacturer followed by single-end (run1 ...
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bioRxiv - Developmental Biology 2021Quote: ... The circRNA sequencing libraries were then generated using TruSeq Stranded Total RNA HT Sample Prep Kit (Illumina RS-122-2203) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... 10%(v/v) N,N-dimethyl formamide) and 1 μl of Tagment DNA Enzyme from Nextera Sample Preparation Kit (Illumina) were added to the DNA-beads complex and incubated for 70 sec at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Long mRNA libraries (2 replicates per time-point) were built using the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina). Library concentration was assessed for all libraries using the Qubit fluorimetric system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq was carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide for the Illumina HiSeq 1000 System (Illumina) and the KAPA Library Quantification Kit (Kapa Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an indexed library per sample was constructed using the NEXTERA XT DNA library prep kit (Illumina, San Diego, CA, USA) and sequenced in an Illumina MiSeq platform at Sistemas Genómicos (Valencia ...
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bioRxiv - Evolutionary Biology 2021Quote: Sequencing of samples was conducted by the University of Chicago Genomics core using an Illumina HiSeq2500 (Illumina, San Diego, CA) with paired-end 100 base pair reads ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were sequenced on an Illumina HiSeq 4000 producing 2×150 bp paired-end reads (Illumina, San Diego, CA, USA) at Novogene (Sacramento ...
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bioRxiv - Developmental Biology 2020Quote: ... was isolated from pooled E8.0-E8.5 stage embryos using Isogen II reagent (Nippongene) and subjected to RNA-seq library preparation using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) based on manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The precipitated or input chromatin DNA was purified and converted to barcoded libraries using the TruSeq ChIP Sample Prep Kit (Illumina). Then 101 bp paired-end sequencing was performed on the HiSeq 4000 (Illumina).
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bioRxiv - Developmental Biology 2020Quote: ... adapter indexed strand-specific RNA-seq libraries were generated from 1000 ng of total RNA following the dUTP method using the stranded mRNA LT sample kit (Illumina). Libraries were pooled and sequenced on Illumina HiSeq2500 in 75bp paired-end mode ...
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bioRxiv - Cell Biology 2022Quote: ... The generated data were subsequently demultiplexed using sample-specific barcodes and changed into fastq files using bcl2fastq (Illumina; version 1.8.4). Reads were afterwards aligned to the human reference genome (GRCh38/hg38 ...
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bioRxiv - Physiology 2022Quote: ... Sequencing libraries were prepared from 100 ng of total RNA using the TruSeq Stranded Total RNA sample preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 200 ng of total RNA was subsequently used to prepare RNA-Seq library by using TruSeq RNA sample prep kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and germfree C57BL/6J mice were prepared using the Clonetech SMARTer Ultra Low Input library preparation kit combined with Nextera XT DNA sample preparation kit (Illumina) and sequenced with single-end 100 bp on a HiSeq2000 platform at the UNC High Throughput Sequencing Core Facility ...
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bioRxiv - Molecular Biology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: 25.000 FACS-sorted cells were collected and used for ATAC Library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). The cell pellet was resuspended in 50 µl Lysis/Transposition reaction (12.5 µl THS-TD-Buffer ...
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bioRxiv - Immunology 2022Quote: ... Approximately 1ug of total RNA was used as an input material for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). Size of the libraries was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Total RNAs samples (3 μg) were depleted from ribosomal RNA using the bacteria Ribo-Zero® rRNA Removal kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: 500 ng of RNA were used to prepare sequencing libraries using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 & -2002, Illumina). Libraries were pooled into five master mixes containing 12 or 16 samples ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared by the Purdue Genomics Core Facility using the Truseq DNA PCR-Free Sample Preparation Kit (Illumina). Whole-genome sequencing was performed using Illumina short-read technology (2×100bp ...
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bioRxiv - Genomics 2020Quote: Mate pair sequencing library was prepared using Illumina-compatible Nextera Mate Pair Sample Preparation Kit (Illumina Inc., Austin, TX, U.S.A.). About 4 μg of genomic DNA was simultaneously fragmented and tagged with mate pair adapters in a transposon based tagmentation step ...