Labshake search
Citations for Illumina :
2951 - 3000 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... and ligated with the appropriate indexed adaptors using the TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, San Diego, CA). The quality of library was validated by an Agilent Bioanalyzer ...
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bioRxiv - Genomics 2022Quote: All samples (n=75) underwent 16S rRNA gene sequencing using 515F-806R primers on the MiSeq (Illumina, San Diego, USA) as previously described (Seaton et al. ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using Nova Seq S4 (run type: PE100, type of library: NexteraXT, 25 million reads per sample; Illumina). Preprocessing and analysis were done using Galaxy Quality and adapter trimming of reads was performed using RNA Galaxy workbench 2.058 ...
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bioRxiv - Developmental Biology 2022Quote: ... Clustering of the index-coded samples was performed using a cBot cluster generation system and the HiSeq PE Cluster Kit v4-cBot-HS (Illumina). Then ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample libraries were prepared for sequencing according to the HiSeq Reagent Kit Preparation Guide (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... We stored the DNA at −80° C and an aliquot from each sample was used for sequencing using a MiSeq sequencer (2 × 250 bp, Illumina). A first run was sent to the Integrated Microbiome Resource (Halifax ...
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bioRxiv - Microbiology 2023Quote: ... Samples were DNAse-treated and sent for library preparation and mRNA-seq by NUcore (Northwestern) on a HiSeq 4000 (Illumina) with 50 bp single-end reads ...
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bioRxiv - Genomics 2023Quote: ... all samples were pooled at 4 nm concentration and paired-end [300bp (2 × 151bp)] sequenced in MiSeq platform (Illumina, USA).
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bioRxiv - Neuroscience 2023Quote: ... performed paired-end next-generation RNA sequencing with a depth of 60 million reads per sample on the NovaSeq platform (Illumina) at the University of Michigan Advanced Genomics Core ...
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Molecular identification of ALDH1A1 and SIRT2 in the astrocytic putrescine-to-GABA metabolic pathwaybioRxiv - Neuroscience 2023Quote: ... The sample libraries were prepared for sequencing according to the HiSeq Reagent Kit Preparation Guide (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... and paired-end transcriptome sequencing of each sample was conducted using an Illumina NovaSeq6000 sequencer (Illumina, San Diego, CA, USA). Over a total of 30 million 101 base paired-end reads were obtained (Suppl ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing was either performed using the MinION sequencer (Oxford Nanopore Technologies) or with the Nextera XT DNA Sample Preparation kit (Illumina) as described elsewhere (49 ...
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bioRxiv - Microbiology 2023Quote: ... DNA-Seq libraries were prepared using Nextera XT library preparation kit with 700 pg DNA input per sample and 6:30 min tagmentation at 55 °C and barcoded using Nextera XT indexes (Illumina). RNA-Seq libraries were prepared using KAPA RNA HyperPrep kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Resulting cDNA was amplified using 10 cycles and the amplified product was used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina). Finally ...
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bioRxiv - Physiology 2023Quote: ... The subsequent steps of library preparation were carried out on the Agilent NGS Bravo workstation in 96-well plates adapted for the TruSeq PCR-free Sample Preparation protocol by Illumina.
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bioRxiv - Microbiology 2023Quote: ... 3-5 µg of each sample was treated with Ribozero® rRNA Removal Kit according to the manufacturer’s instructions (Illumina, USA ...
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bioRxiv - Developmental Biology 2023Quote: ... Illumina sequencing libraries were prepared from the amplified cDNA from each sample group using the Nextra DNA library prep kit (Illumina) and then sequenced on Illumina HiSeq 2500 Rapid flowcells (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Input and RNA eluted from m6A-IP were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). All sequencing was performed on an Illumina HiSeq 6000 according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... The PhiX Control library was spiked in the sequencing sample at 10% (v/v) (Illumina, cat. no. FC-110-3001).
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from the collected leaf samples using the Dynabeads mRNA DIRECT Kit (Illumina, San Diego, CA, USA) and quantified and assessed for integrity using a NanoDrop spectrophotometer and an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries with unique dual indexes were pooled and sequenced on partial lanes targeting ∼50M reads per sample on a HiSeq or a NovaSeq 6000 sequencer (Illumina) using 150-bp paired-end sequencing chemistry.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then depleted of rRNA and converted to cDNA libraries using the TrueSeq® Stranded Total RNA kit (Illumina). cDNA libraries were sequenced with the HiSeq 4000 Sequencing System (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... We then made a uniquely indexed library from each sample using the Illumina TruSeq® Stranded mRNA library kit (Illumina). We also separately made a uniquely indexed miRNA library from each sample using the Illumina Small RNA library kit (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... We also separately made a uniquely indexed miRNA library from each sample using the Illumina Small RNA library kit (Illumina). We made an equimolar pool of the mRNA libraries and a separate equimolar pool of the miRNA libraries ...
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bioRxiv - Microbiology 2023Quote: ... Bar-coded libraries were prepared from each genomic DNA sample using a TruSeq DNA kit according to manufacture specifications (Illumina), and the samples were subjected to Illumina-based whole-genome sequencing using a MiSeq 250 paired-end run (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... was performed at 10 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina) using High output sequencing kits ...
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bioRxiv - Microbiology 2023Quote: The extracted DNA samples were prepared for Illumina next-generation sequencing using the NEBNext Ultra II FS DNA Prep kit from Illumina, part number E7805 ...
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bioRxiv - Cell Biology 2023Quote: ... The generated data were subsequently demultiplexed using sample-specific barcodes and changed into fastq files using bcl2fastq (Illumina; version 1.8.4). Reads were afterwards aligned to the human reference genome (GRCh38/hg38 ...
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bioRxiv - Genomics 2023Quote: ... The pooled libraries were sequenced in the 2 × 150bp format to an average depth of 300 million reads/sample on a NovaSeq 6000 S4 (Illumina) by Psomagen Inc.
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bioRxiv - Molecular Biology 2023Quote: Libraries were prepared from the rRNA-depleted or Terminator-digested RNAs using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) and the IDT for Illumina – TruSeq RNA UD indexes (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... All eighteen samples were sequenced as a multiplexed library on a single lane of a HiSeq 4000 sequencing system (Illumina), generating 200 nt paired end reads.
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bioRxiv - Plant Biology 2023Quote: ... Plate-based RNA sample preparation was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina’s TruSeq Stranded mRNA HT sample prep kit utilizing poly-A selection of mRNA following the protocol outlined by Illumina in their user guide ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina indexed adapters were added to the DNA on the M-280 beads using the TruSeq Library Sample Preparation kit (Illumina) as follows ...
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bioRxiv - Genomics 2023Quote: ... Single-end reads of 75 basepairs were generated for each sample on an Illumina NextSeq 500 (Illumina, San Diego, CA). Raw data files were uploaded to Gene Expression Omnibus with accession number GSE216035.
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bioRxiv - Neuroscience 2023Quote: ... Preparation of the RNA sample library and RNA-seq were performed by the Genomics Core Laboratory at Weill Cornell Medicine using Illumina Stranded mRNA Sample Library Preparation kit (Illumina), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... under each condition (30Mb read/each sample) was loaded on the Illumina Nextseq 500 system with a NextSeq 500/550 High Output Reagent CartriDEG v2 (Illumina) for single-end reads at Carnegie Embryology Department’s in-house sequencing facility ...
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bioRxiv - Immunology 2023Quote: ... was performed at 19-36 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina) with 1 High Output Kit v2.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina). After cluster generation ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of PCR product for each sample were normalized in 30 µl volume for NGS library construction using Illumina DNA Prep (M) Tagmentation kit (Illumina). IDT for Illumina Nextera DNA Unique Dual Indexes were used for pooling multiple samples together ...
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bioRxiv - Cancer Biology 2024Quote: ... Equimolar libraries from each sample were pooled and subjected to 75-bp paired-end sequencing on a NextSeq 550 machine (Illumina). Raw reads were demultiplexed and FASTQ files were generated using Bcl2fastq v2.20.0.422 (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... 0.5 μg of total RNA was used as an input material for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). Library size was confirmed using a Fragment Analyzer (Advanced Analytical Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... was performed at 25 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina) using High output sequencing kit ...
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bioRxiv - Neuroscience 2022Quote: ... The Genomics Facility at the Cornell Institute of Biotechnology used 500ng of RNA/sample for 3’RNA library preparation with the Lexogen QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Illumina), sequenced libraries on an Illumina NextSeq500 sequencer (single end 1×86bp) ...
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bioRxiv - Molecular Biology 2022Quote: ... A paired-end library (insert size of 460 bp for each sample) was constructed using the TruSeq Nano DNA Library Prep kit (Illumina), and high-throughput sequencing was performed on an Illumina HiSeq 2500 sequencer with a HiSeq Rapid v2 Reagent Kit (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were pooled from different samples based on molar concentrations and sequenced with 1% PhiX spike-in on a NextSeq 500 instrument (Illumina) with 33 bases each for read 1 and read 2 ...
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bioRxiv - Microbiology 2022Quote: ... 5– 10 × 106 50-bp paired-end reads were obtained for each sample on an Illumina HiSeq 2500 using the TruSeq SBS Kit v3 (Illumina). Post-run demultiplexing and adapter removal were performed and FASTQ files were inspected using fastqc (Andrews ...
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bioRxiv - Neuroscience 2022Quote: ... We pooled libraries from different samples based on molar concentrations and sequenced them on a NextSeq 500 or NovaSeq instrument (Illumina) with 28 bases for read 1 ...