Labshake search
Citations for Illumina :
2801 - 2850 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... were prepared at the Virginia Tech Genomics Core Sequencing Laboratory using the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina), which uses polyA enrichment ...
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bioRxiv - Plant Biology 2021Quote: ... was performed with two (for HB40-IOE seedlings) and three (for WT and HB40OX seedlings) biological replicates per sample on HiSeq4000 (Illumina). The sequencing adaptors and low-quality bases were trimmed using Trimmomatic v0.38 (Bolger et al ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared using either the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina) or KAPA Stranded mRNA-Seq Library Prep kit (KAPA Biosytems ...
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bioRxiv - Microbiology 2020Quote: RNA-seq libraries (strand-specific, paired end) were generated from total fungal RNA using the TruSeq RNA sample prep kit (Illumina). Seventy-five nucleotides of the sequence were determined from both ends of each cDNA fragment using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... and then ribosomal RNA was removed from the samples by the Ribo-Zero kit (Illumina, San Diego, CA, United States). The sequencing library was prepared following the standard protocol for TruSeq Stranded Total RNA Sample Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... The generated data were subsequently demultiplexed using sample-specific barcodes and changed into fastq files using bcl2fastq (Illumina; version 1.8.4). Reads were afterwards aligned to the human reference genome (GRCh38/hg38 ...
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bioRxiv - Cancer Biology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Total 500 ng RNA processed for library preparation using “TruSeq” RNA sample preparation-V2 kit and “Ribo-Zero” rRNA removal kit (Illumina) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... The preparation of RNAseq libraries were performed using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) at Novogene Co. ...
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bioRxiv - Cancer Biology 2020Quote: ... These samples were subsequently sequenced in a paired end 151 bp run on a Novaseq instrument (Illumina, San Diego, CA) resulting in an average coverage of 120x.
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bioRxiv - Cancer Biology 2021Quote: ... Illumina-stranded total RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Paired-end libraries with 2 × 75 bp reads were prepared from the extracted RNA using the TruSeq Stranded total RNA LT Sample Prep (RiboZero Gold, Illumina) using Illumina methodology ...
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bioRxiv - Evolutionary Biology 2022Quote: ... all the replicates were pooled by sample before purification and to proceed to the further preparation of the library according to the protocol outlined by Illumina (« 16S metagenomic Sequencing Library Preparation ») ...
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bioRxiv - Molecular Biology 2022Quote: ... Both 5’-tir-P enriched and oxidized samples were subjected to a sRNA library construction by the TruSeq Small RNA Library Preparation kit (Illumina). The resulting libraries were sent to BGI (Hongkong ...
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bioRxiv - Microbiology 2022Quote: ... Sequence libraries were prepared from the extracted DNA samples using the Nextera DNA Flex Library Prep Kit (Illumina, Cat. # 20018705) following a low volume variation of the standard protocol.29
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bioRxiv - Microbiology 2022Quote: ... plate-based RNA sample prep was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina Ribo-Zero rRNA Removal Kit (Bacteria) and the TruSeq Stranded Total RNA HT sample prep kit following the protocol outlined by Illumina in their user guide ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cDNAs from individual cells were used for the construction of NGS libraries with the Nextera XT DNA sample preparation kit (Illumina). Libraries were pooled ...
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bioRxiv - Immunology 2022Quote: ... 1000ng RNA from each sample was used as a template for preparing Illumina compatible libraries using the TruSeq RNA Library Prep Kit v2 (Illumina). Library sizes were checked using D5000 high sensitivity tape on the TapesSation 2200 (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... Dual-index paired-end sequencing of the throat samples was performed on the V4 region of the 16S rRNA gene on a MiSeq Desktop sequencer (M00984, Illumina), as previously described (De Boeck et al ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries were prepared using the Nextera XT sample prep kit and sequenced on the Illumina MiSeq platform (Illumina) with 300-bp paired-end read configuration (MiSeq Reagent Kit v3) ...
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bioRxiv - Microbiology 2022Quote: ... Messenger RNA was enriched by first depleting ribosomal RNA from samples using the Ribo-Zero rRNA removal (bacteria) magnetic kit (Illumina). The purified mRNA was prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer’s instructions in combination with AMPure XP reagent for clean-up of adaptor-ligated DNA ...
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bioRxiv - Microbiology 2022Quote: ... The generated data were subsequently demultiplexed using sample- specific barcodes and changed into fastq files using bcl2fastq (Illumina; version 1.8.4). The quality of the data was assessed using FastQC (v0.11.8 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... Further library preparation of rRNA-depleted samples was performed using TruSeq Stranded mRNA library preparation kit (Illumina, RS-122-2101) according to the manufacturer’s instructions apart from omitting the initial steps for mRNA pull-down ...
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bioRxiv - Molecular Biology 2022Quote: ... The samples were pre-amplified with the Nextera Index kit and the Nextera DNA Library Prep kit (both from Illumina), incorporating index sequences at the ends ...
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bioRxiv - Immunology 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The library samples were then diluted to the final concentration of 10nM and loaded onto two lanes of the NovaSeq 6000 (Illumina) instrument flow-cell for a 100-cycle sequencing run ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated from 1 μg of total RNA using a TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples) (Illumina) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot- HS (Illumina) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: One μg of total RNA of high quality (RIN>8) was used for sequencing with a TruSeq RNA sample preparation kit (Illumina). We here included 99 islet samples in addition to the 89 islet samples and processed them uniformly following the same protocol as described previously (8) ...
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bioRxiv - Microbiology 2020Quote: Clustering of index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Sequencing libraries were prepared with the Illumina TruSeq stranded mRNA sample preparation kit according to the manufacturer’s protocol (Illumina, USA). In brief ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina), and the library preparations were then sequenced on an Illumina NovaSeq 6000 platform and 150 bp paired-end reads were generated ...
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bioRxiv - Developmental Biology 2021Quote: ... sRNA libraries for dicer-null embryos were prepared using Illumina TruSeq Small RNA Sample Prep kit (Illumina, RS-200-0012) and sequenced on HiSeq 50bp single-end sequencing platform ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of RNA per sample was used to prepare a dual-indexed strand-specific cDNA library using TruSeq Stranded mRNA library Prep Kit (Illumina). The resulting libraries were assessed for their quality and size distribution using Qubind Agilent 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing of 12 liver samples (with 84 unrelated samples) was performed using a single NovaSeq S1 200 cycle sequencing run on a NovaSeq 6000 machine (Illumina) through IMB Sequencing Facility ...
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bioRxiv - Plant Biology 2021Quote: ... Samples with a RIN value of > 7 were used for library preparation (Illumina TruSeq Stranded Total RNA kit, Illumina, USA) and sequenced on an Illumina HiSeq 4000 to obtain 150bp paired-end reads ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared for each product of long PCR (ranging from 15 to 33 kb) using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq platform to generate paired-end (PE ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA samples with RIN>8.0 were shipped to Beijin Genome Institute for a library preparation and sequencing (Illumina HiSeq 2500). Integrative Genomics Viewer (2.4.14 Broad Institute ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting libraries were minimally amplified to enrich for fragments using adapters on both ends and then quantified for sequencing at eight samples/flow cell by using a NextSeq 500/550 (Illumina) sequencer (PE 150).
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bioRxiv - Genomics 2020Quote: ... Wild-type cultured aortic ECs were used to generate the immunoprecipitated and input control libraries using TruSeq DNA Sample Prep Kit v2 (Illumina) following manufacturer’s direction ...
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bioRxiv - Microbiology 2021Quote: ... 5–10 × 106 50-bp paired-end reads were obtained for each sample on an Illumina HiSeq 2500 using the TruSeq SBS Kit v3 (Illumina). Post-run demultiplexing and adapter removal were performed and fastq files were inspected using fastqc (Andrews S ...
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bioRxiv - Microbiology 2021Quote: The second stage PCR procedure attached unique index primers to each sample using the Nextera XT v2 set A kit (Illumina). Purified DNA (5 μl ...
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bioRxiv - Microbiology 2021Quote: ... UK). Libraries were prepared from 8 samples (4× T. brucei, 4× T. congolense) using the TruSeq Stranded mRNA kit (Illumina) and 2 × 75 bp paired-end sequencing was carried out using a HiSeq 4000 system (Illumina) ...
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bioRxiv - Immunology 2020Quote: RNA-seq libraries were constructed from up to100 ng of total RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina). Libraries were clustered on a flowcell using the TruSeq Paired-end Cluster Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Two paired-end Illumina libraries containing 250 and 600 bp insertions (DRR029525 and DRR029526) were constructed using a TruSeq DNA Sample Prep Kit (Illumina). Three mate-pair libraries with 3000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end Illumina library (DRR029460; see also Supplementary File 1) was constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and an RNA sample extracted from an E ...
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bioRxiv - Microbiology 2021Quote: ... All RNA-seq and RIP-seq experiments were performed thrice with RNA samples obtained from independently prepared mycelia using the NextSeq 500 system (Illumina) and mapped to the A ...
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bioRxiv - Immunology 2021Quote: ... Libraries were sequenced by paired end (2x75 bp) to a depth of 25 million reads per sample (Illumina Miseq v3).