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Citations for Illumina :
2651 - 2700 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The RNA-seq transcriptome libraries were constructed using the TruSeqTM RNA sample preparation kit (Illumina, San Diego, CA, USA) with a total of 2 µg RNA ...
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bioRxiv - Immunology 2024Quote: ... cDNA was prepared using the TruSeq Stranded Total RNA Sample Preparation kit with Ribo Zero Gold (Rev. E; Illumina) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... RNA samples with RIN > 8 were used for the library preparation using TruSeq stranded total RNA kit (Illumina, 15035748). Yield of final prepared libraries were quantified in Qubit 4.0 fluorometer (Thermofisher ...
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bioRxiv - Genomics 2024Quote: ... We added a FORMAT (GT) and SAMPLE (0/1) column to the VCF and used hap.py (https://github.com/Illumina/hap.py) to compare the v4.2.1 benchmark VCF and BED (https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/ChineseTrio/HG005_NA24631_son/NISTv4.2.1/GRCh38/) ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries were constructed using the TruSeq ChIP Sample Preparation Kit (Cat #, IP-202-1012; Illumina, San Diego, CA), and single-end reads (50 nt ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were generated using the Illumina Tru-Seq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Multiplexed samples were subsequently pooled and further prepared for sequencing using the Nextera XT DNA library prep kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... RNA sequencing libraries were constructed from extracted RNA samples which passed quality control using TruSeq Stranded mRNA Kits (Illumina). The resulting libraries were pooled and sequenced using an Illumina NovaSeq6000 S4 v1.5 with 2 x 150 base pair read lengths and a target read depth of 20 million total fragments sequenced per library ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was carried out using the TruSeq DNA PCR-free Sample Preparation Kit (Illumina Inc., San Diego, USA), followed by library quantification using Qubit and q-PCR ...
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bioRxiv - Microbiology 2024Quote: ... Samples were pooled in groups of 96 and then checked on a MiSeq Nano (Illumina Inc., San Diego CA) for pool balance ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of isolated genomic DNA sample and 100 nmol of each primer (WISH_Illumina_fwd and WISH_Illumina_rev, see Table S6) were used in a DreamTaq MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of isolated genomic DNA sample and 100 nmol of each primer (WISH_Illumina_fwd and WISH_Illumina_rev, see Table S11) were used in a DreamTaq MasterMix (Thermo Fisher Scientific) ...
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Genetic underpinnings of predicted changes in cardiovascular function using self supervised learningbioRxiv - Systems Biology 2024Quote: ... Libraries were sequenced to a minimum depth of 5M reads per sample on a NovaSeq 6000 instrument with a NovaSeq S1 v1.5 100-cycle kit (Cat# 20012865; Illumina).
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bioRxiv - Genomics 2024Quote: ... Each sample was sequenced to a target depth of 37.5 million read pairs using a NovaSeq 6000 device (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... mRNA libraries were prepped using poly-A pull-down to enrich mRNAs from total RNA samples followed by Illumina TruSeq Stranded mRNA Library prep (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: Barcoded libraries were prepared from 0.5 µg of total RNA using the TruSeq RNA Sample Preparation v2 kit (low-throughput protocol, Illumina) and quantified with the Bioanalyzer system (Agilent) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Library preparation for RNA sequencing (RNAseq) was performed using a TruSeq stranded mRNA sample prep kit (Illumina, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... on an Illumina NovaSeq 6000 with an expectation of a minimum number of 1.5 million read pairs per sample. We used Stacks v.2.62 (Catchen et al. 2011, 2013) to build loci from Illumina reads ...
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bioRxiv - Cancer Biology 2024Quote: Libraries were prepared from 500 ng of each total-RNA sample using the TruSeq Stranded mRNA Library Kit (Illumina) according to the provided protocol and purified using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were prepared with TruSeq Stranded Total RNA Library Prep with ribosomal RNA depletion for all samples according to the manufacturer’s instructions (Illumina). The quality of the extracted RNA and cDNA at each library preparation step was assessed with Qubit (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Per-cell gene expression was quantified for each sample using the BaseSpace® SureCellTM RNA Single-Cell Analysis Workflow v1.2.0 from Illumina, which uses Isas v1.2.6-000184develop for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA libraries were prepped using poly-A pull-down to enrich mRNAs from total RNA samples followed by Illumina TruSeq Stranded mRNA Library prep (Illumina ...
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bioRxiv - Systems Biology 2024Quote: The libraries were mixed and sequenced pair-ended at 50-bp read length on an Illumina NextSeq 2000 sequencer to depths of 14.1-351.8 million reads per eluate sample and 3.14-16.45 millions reads per input sample using the NextSeq 1000/2000 P2 Reagents (100 Cycles) kit (Illumina 20046811). Reads were demultiplexed and adapters were removed using Cutadapt85 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA libraries were constructed for each RNA sample using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Library construction was then performed using the TruSeq DNA PCR-Free HT sample prep kit (Illumina, San Diego, CA), followed by sequencing on a HiSeq 2500 system ...
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bioRxiv - Cancer Biology 2024Quote: Library preparation for both human and mouse samples involved using the TruSeq stranded total-RNA Library Preparation kit (Illumina) with 1 μg of RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Restrictions limiting the amount of material collected from the Endolithic environment meant that samples for metatranscriptomic (Illumina short read) and metabolomic (polar LC-MS ...
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bioRxiv - Systems Biology 2024Quote: ... cDNA libraries were generated from isolated RNA samples using the Total RNA Prep with Ribo-Zero Plus kit (Illumina). Barcoded libraries were sequenced on an Illumina NovaSeq instrument [150 nt paired-end reads to a depth of 1.93 × 108 ± 7.94 × 107 reads/sample (mean ± SD) ...
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bioRxiv - Cell Biology 2024Quote: ... Illumina transcriptome library preparation and sequencing total RNA samples were prepared for mRNA-sequencing using the Illumina Stranded mRNA Ligation Sample Preparation kit following the manufacturer’s protocol (Illumina). Libraries were validated for length and adapter dimer removal using the Agilent TapeStation 4200 D1000 High Sensitivity assay (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... We prepared the library from RNA samples with an RNA integrity number (RIN) greater than 7.0 using the TruSeq Stranded mRNA Library Prep Kit (Illumina), according to the recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were sequenced with a depth of at least 50,000 reads per spot (minimum of ∼250 million/sample) at the NovaSeq (Illumina). The Visium Human Transcriptome Probe Set v1.0 contains probes to 19,144 genes and after computational preprocessing (filtering for probes off-target activity ...
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bioRxiv - Genetics 2024Quote: ... 5 µg of total RNA for each sample was then processed using the Tru-Seq stranded mRNA kit (Illumina) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits (RS-122-2101, Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR products were gel purified and stored in 50 μL buffer containing 10mM Tris (pH 8.0) until submission to TruSeq® DNA library preparation (Illumina) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were centrifuged at 500 g for 10 min and immediately resuspended in 25 µl of buffer containing 2.5 µl of Tn5 transposase (Illumina, #15027865) for a 30 min incubation at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Purified cells were lysed in ATAC lysis buffer for 5 min to get nuclei and then transposed with Tn5 transposase (Illumina) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no ...
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bioRxiv - Cancer Biology 2021Quote: ... with some modifications based on Corces et al.59 using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: A NextSeq run was completed on the pooled libraries using the NextSeq 500 hi-output v2 75-cycle kit and Buffer Cartridge (Illumina). Sequence files were downloaded from the MIP NGS server ...
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bioRxiv - Systems Biology 2020Quote: For library preparation up to 700 pg cDNA was desiccated and rehydrated in 1 µl Tagmentation mix (1x TruePrep Tagment Buffer L, 0.1 µl TruePrep Tagment Enzyme V50; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented at 55°C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... A NextSeq run was completed on the pooled libraries using the NextSeq 500 hi-output v2 75-cycle kit and Buffer Cartridge (Illumina). Sequence files were downloaded from the NGS server ...
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bioRxiv - Biochemistry 2022Quote: ... 50 ng of DNase I treated total RNA and three-stage purified mRNA from the two biological replicates were fragmented using the TruSeq RNA Library Prep Kit v2 fragmentation buffer (Illumina). First-strand cDNA synthesis was performed using the random hexamer primer ...
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bioRxiv - Molecular Biology 2022Quote: ... were resuspended in 50 μl transposase mixture (25 μl Illumina TD buffer, 22.5 μl H2O, and 2.5 μl Illumina TDE1 transposase) and incubated at 37°C for 30 min with shaking at 1,000 rpm ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclear pellets were then resuspended in 50 μl transposition reaction mix (25 μl TD buffer (Nextera DNA Library Prep Kit, Illumina), 2.5 μl TDE1 enzyme (Nextera DNA Library Prep Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... which were then fragmented randomly and used for cDNA generation and subsequent second-strand synthesis using a custom second-strand synthesis buffer (Illumina), dNTPs ...
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bioRxiv - Genetics 2020Quote: ... We then resuspended the nuclear pellet in 50 μl of transposition reaction mix (25 μl Buffer TD, 2.5 μl TDE1 (Illumina 15028212); 7.5 μl water ...
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bioRxiv - Genomics 2020Quote: ... The normalised samples (4 nM) were denatured with 0.2 N NaOH and diluted 20 pM using pre-chilled Hybridisation Buffer (HT1) (Illumina, USA). The 20 pM transcriptome libraries were further diluted to 10 pM with pre-chilled HT1 buffer prior to whole transcriptome sequencing on a MiSeq platform.
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bioRxiv - Immunology 2021Quote: For library preparation up to 700 pg cDNA was desiccated and rehydrated in 1 µl Tagmentation mix (1x TruePrep Tagment Buffer L, 0.1 µl TruePrep Tagment Enzyme V50; from TruePrep DNA Library Prep Kit V2 for Illumina; Vazyme) and tagmented at 55°C for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl amplified cDNA was tagmented in 1x Tagmentation Buffer using 0.8 μl bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Microbiology 2022Quote: ... ATAC-seq libraries were prepared from 50,000 cells per replicate using Illumina Tagment DNA Enzyme and Buffer Kit (Illumina 20034197) according to the Omi-ATAC-seq protocol (Corces et al. ...