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Citations for Illumina :
2901 - 2950 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Physiology 2021Quote: ... Single-indexed strand-specific cDNA library from total RNA samples (500 ng input with RIN ≥ 5) was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) as per directions by the manufacturer ...
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bioRxiv - Physiology 2021Quote: ... Poly-A enriched complementary DNA (cDNA) libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 μg of purified RNA for each sample was processed using the Ribozero rRNA Removal Kit (Gram-negative bacteria) (Illumina) according to the manufacturer’s instructions except that reaction volumes were reduced by 50% ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA samples were processed further at the Barts and the London Genome Centre and run on Infinium MethylationEPIC arrays (Illumina).
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bioRxiv - Evolutionary Biology 2020Quote: ... and mate-pair libraries of 3 kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We then assessed library quality using the HS DNA Kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... The quality of libraries was assesses based on size distribution and concentration using 2100 Bioanalyzer with DNA 1000 chip (Agilent Technologies. All samples were sequenced 25M reads on an Illumina NovaSeq 6000 Sequencer using the 2×50bp paired-end platform.
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bioRxiv - Microbiology 2021Quote: ... A second PCR reaction was performed according to the manufacturers protocol to obtain sequence libraries with adaptor sequences from the TruSeq DNA LT Sample Prep Kit (Illumina). PCR products were purified using magnetic Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: Libraries were prepared for 150 bp paired-end sequences using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, CA, USA). Namely ...
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bioRxiv - Cancer Biology 2021Quote: ... Barcoded libraries of ten samples (4 treated, 4 untreated and two controls) were equimolarly pooled and sequenced on MiSeq (Illumina) using MiSeq v3 150 reagent kit (MS-102-3001 ...
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bioRxiv - Genomics 2020Quote: ... Replicas of each 384 sample pools were loaded onto the S4-flow cell following NovaSeq-XP workflow as per the manufacturer’s instructions (Illumina Inc). Dual indexed single end sequencing with 36bp read length was carried out on NovaSeq 6000 platform.
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bioRxiv - Cancer Biology 2021Quote: ... 9 samples with DNA >40kbp were concurrently sequenced using PacBio and Illumina and the remaining 13 tumor samples were sequenced by Illumina WGS only ...
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bioRxiv - Cancer Biology 2021Quote: ... sequencing of samples for which RNA of sufficient quality and quantity was available was performed on a NextSeq 500 instrument (Illumina) as previously described 17 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using Smart-Seq2 64 and modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina) with 300 pg of cDNA as input material ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ug of DNA was processed according to manufacturer instructions (Paired-End DNA sample Prep Kit – Illumina – PE-930-1001), except that DNA was ligated to custom-made adapters for 4 hours at RT ...
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bioRxiv - Microbiology 2021Quote: ... Samples were sequenced using an Illumina MiSeq for 250 X 250 bp paired-end sequencing (Illumina, San Diego, CA, USA). Samples were demultiplexed by the facility ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing libraries were prepared by LC Sciences following Illumina’s TruSeq-stranded-total-RNA-sample preparation protocol (Illumina, San Diego, USA). Quality control analysis and quantification of the sequencing library were performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip ...
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bioRxiv - Genomics 2021Quote: ... RNA sample preparations were performed as described previously [22] using the ARTseq™ and Epicentre’s Ribo-Zero™ kits (Illumina). Briefly ...
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bioRxiv - Genomics 2020Quote: ... DNA pools and from one single individual of each sex were constructed using the Genomic DNA Sample Preparation Kit (Illumina) according to manufacturer’s instructions and sequenced on the Illumina HiSeq2000 platform (Illumina ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... Approximate 1.5pM of the library for each sample was loaded onto a NextSeq 500 system and sequenced using High Output sequencing kits (75× 2bp) (Illumina). After discarding the cells with high mitochondrial gene ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared from 50 ng of phage genomic DNA using the Nextera DNA Sample Preparation Kit (Illumina, USA) at the University of Cambridge Sequencing Facility ...
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bioRxiv - Microbiology 2020Quote: ... The marbled eel adomavirus RCA products and discus total DNA were prepared with a Nextera XT DNA Sample Prep kit and sequenced using the MiSeq (Illumina) sequencing system with 2 × 250 bp paired-end sequencing reagents ...
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bioRxiv - Immunology 2021Quote: ... 1 ng of cDNA was used to prepare a standard Nextera XT sequencing library (Nextera XT DNA sample preparation kit and index kit, Illumina). Whole-transcriptome amplification and sequencing library preparations were performed in a 96-well format to reduce assay-to-assay variability ...
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bioRxiv - Immunology 2020Quote: ... High-quality RNA (approximately 500 ng; RIN 8) was used for nondirectional paired-end mRNA library preparation (TruSeq Sample Preparation Kit; Illumina).
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bioRxiv - Immunology 2020Quote: ... Indexed samples were pooled together for 100 cycles of paired end sequencing on a HiSeq 4000 or HiSeq 2500 system (Illumina). More than 40 million reads per sample were sequenced for downstream analysis.
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared following the RNA-seq sample work flow using the Scriptseq Complete kit for bacteria and indexed with unique Scriptseq Index PCR primers (Illumina).
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bioRxiv - Immunology 2020Quote: ... Sequencing for all other samples was performed according to a 50 bp single-end strategy using the HiSeq2500 sequencer (HiSeq SBS Kit v4; Illumina). Post-sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... libraries were also prepared using 50 ng of gDNA isolated from NaR P5 and 3D-RA DD25 cells by following the Nextera DNA Sample Preparation Guide (Illumina). The libraries were purified using the MinElute PCR purification kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: Libraries for each sample were sequenced on a NextSeq550 75 Cycle High Output sequencing kit (Illumina Inc., Sunnyvale, CA, USA). For each library ...
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bioRxiv - Cell Biology 2021Quote: ... pooled and sequenced 75 bp paired-ended (human samples) or single-end (cultured and sorted fibroblasts) on the NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The index-coded samples were clustered with a cBot Cluster Generation System using a HiSeq 4000 PE Cluster Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries were prepared from 1µg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# FC-121-3001/3002, Illumina Inc.) targeting an insert size of 350bp ...
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bioRxiv - Genomics 2021Quote: Plate-based RNA sample prep was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina’s TruSeq Stranded mRNA HT sample prep kit utilizing poly-A selection of mRNA following the protocol outlined by Illumina in their user guide (https://support.illumina.com/sequencing/sequencing_kits/truseq-stranded-mrna.html ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was performed over two batches using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 & -2002, Illumina). Samples were sequenced to 50 base pairs ...
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bioRxiv - Genomics 2020Quote: Library preparation for bulk RNA-seq was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (except for biopsy 01115149-TA, which was prepared using the unstranded Illumina TruSeq RNA Sample Preparation protocol (Revision A ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with 250 ng of total RNA using Illumina’s Truseq RNA Sample Preparation Kit v2 (Illumina, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Ribosomal RNA depleted strand-specific RNA libraries were generated with the TruSeq Stranded Total RNA sample preparation kit with Ribo-Zero Gold (#RS-122-2301and #RS-122-2302, Illumina) and transcriptome capture based libraries were generated with the TruSeq RNA Access Library Prep Kit (#RS-301-2001 ...
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bioRxiv - Genomics 2020Quote: ... the total DNA was sheared to ~350 bp fragments and the libraries were generated using TruSeq Nano DNA HT Sample preparation Kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... An RNA-seq transcriptome library was prepared using the TruSeq™ RNA sample preparation Kit from Illumina (San Diego, CA) and sequencing was performed on an Illumina Novaseq 6000 by the Shanghai Majorbio Bio-pharm Biotechnology Co. ...
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bioRxiv - Genetics 2020Quote: ... equimolar amounts of amplification products from each sample of the 96-well microtiter plate were bulked and applied to c-Bot (Illumina) bridge PCR followed by sequencing on Illumina Hiseq2000 ...
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bioRxiv - Genetics 2020Quote: ... Samples were multiplexed and paired-end sequencing was performed using either a MiniSeq or NovaSeq 6000 (Illumina; San Diego, CA).
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bioRxiv - Microbiology 2020Quote: ... quantified using the Agilent 2100 Bioanalyzer system and used to generate RNA-seq libraries (strand-specific, paired end) using the TruSeq RNA sample prep kit (Illumina).
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bioRxiv - Immunology 2021Quote: ChIP-seq libraries were prepared for each ChIP from three independent ChIP experiments using TruSeq ChIP Sample Preparation Kit (Illumina) or NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng RNA of input and elutes after RIP were used to generate the library using the TruSeq Stranded RNA Sample Preparation Kit (Illumina) and sequenced on the Illumina HiSeq 3000 platform (Jingneng ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-Seq libraries were generated as described (21) using the TruSeq RNA sample preparation kit (Illumina Inc., San Diego, CA). Sequencing was performed using HiSeq 2000 or 2500 systems (Illumina Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... were used to optimize the cDNA amplicon size followed by adding P5 and P7 sample indexes (used in Illumina sequencers), and Illumina R2 sequence via processes of end repair ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA (rRNA) was subsequently depleted from each RNA sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR-amplified sequencing libraries were prepared from 100ng DNA using the TruSeq Nano DNA sample preparation kit (cat# FC-121-4001/4002, Illumina) targeting an insert size of 350bp ...