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Citations for Illumina :
2451 - 2500 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The chromatin was then tagmented by resuspending beads in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina). Samples were incubated at 37 °C for 10 min and the reaction was terminated by adding 150 µl RIPA buffer ...
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bioRxiv - Genetics 2022Quote: ... Nuclei pellet was obtained by centrifugation at 500g for 5 minutes at 4 degrees and resuspended in 100μl ice-cold 1x TD buffer (20034198, Illumina). About 10K nuclei was used for transposition reaction at 37 degrees for 30 minutes in a thermomixer ...
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bioRxiv - Immunology 2022Quote: ... The nuclear pellet was gently resuspended in a final volume of 50 μl transposase reaction mix (25 μL 2 × TD buffer, 2.5 μL Illumina Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina ...
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bioRxiv - Immunology 2022Quote: ... The nuclear pellet was gently resuspended in a final volume of 50 μl transposase reaction mix (25 μL 2 × TD buffer, 2.5 μL Illumina Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Next, the pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water) ...
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bioRxiv - Pathology 2021Quote: ... with some modifications based on (57) using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... was generated and we incubated 7 µM Tn5059 with 10 µM annealed transposon for 30 min at room temperature and then diluted to 0.7 µM Tn5059 transposome complex with standard storage buffer (Illumina).
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bioRxiv - Genomics 2021Quote: ... Centrifugation at 500 g for 10 minutes at 4°C made a pellet of nuclei, which was resuspended in 50 μL transposition mix (25 μL 2× TD buffer, 2.5 μL transposase (Illumina), 16.5 μL PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and pools were diluted to a final concentration of 1.8 pM in HT1 Hybridization Buffer (Illumina, San Diego, CA) and sequenced on an Illumina NextSeq 500 instrument using the NextSeq 500/550 75-cycle High-Output Kit v2.5.
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bioRxiv - Genomics 2021Quote: ... The cell pellets were then resuspended in 20 μl, 10 μl, and 8 μl of transposition mix (25 μl 2×TD buffer, 2.5 μl Tn5 (Illumina), 16.5 μl PBS (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina, cat. FC-121-1031). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Pelleted nuclei were resuspended in 50 μl of transposition mix (25 μl 2 × TD buffer, 2.5 μl Tagment DNA enzyme (Illumina Tag DNA Enzyme and Buffer Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... were used for a single purification and the DNA was eluted using 15 µl of resuspension buffer (RSB, Illumina). After the addition of 1 µl primer mix (25 mM each ...
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bioRxiv - Developmental Biology 2023Quote: ... Beads were washed with Tagmentation Wash Buffer (10mM Tris, pH 7.5, 5mM MgCl2) and incubated with Tn5 transposes (Illumina Tagment DNA Enzyme and Buffer Small Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 50 μl of transposition mixture (25 μl of 2× Illumina Tagment DNA buffer, 2.5 μl of Illumina Tagment DNA enzyme ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was removed and the nuclei were resuspended in 50 μl of transposition mixture (25 μl 2xTD buffer, 2.5 μl transposase from the Illumina Tagment DNA Enzyme and Buffer Kit #20034197 ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was resuspended in transposition reaction mix (12.5 μl TD-Buffer, 1.25 μl Tn5, 11.25 μl water) (Nextera DNA Library Prep Kit, Illumina, Cat# 15028212) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 50 μl of transposition reaction mix (25 μl of Tagmentation DNA buffer, 2.5 μl Tagment DNA enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits ...
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bioRxiv - Genomics 2023Quote: ... tn5-digested ‘input’ controls were made by performing tagmentation for 50 ng iXist-ChrX genomic DNA by a basic 50 μl transposition mix of 1X TDE buffer and 2.5 μl TDE1 Enzyme (Illumina) in H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Pelleted nuclei were incubated in transposase reaction mix (1 X TD reaction buffer, 2.5 µl Tn5 transposase (Nextera, Illumina)) for 30 min at 37 °C ...
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bioRxiv - Genomics 2023Quote: ATAC-seq libraries were prepared from 25,000 cells following the Omni-ATAC protocol 46 and using the Tagment DNA Enzyme and Buffer Kit (Illumina). Samples were pooled and run in one HiSeq lane.
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bioRxiv - Developmental Biology 2023Quote: ... The nuclei were subjected to a transposition reaction with Illumina’s Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, 20034197) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 µl of Tagment DNA buffer 1.25 µl of Amplicon Tagment Mix (Nextera XT kit, Illumina FC-131-1096) were added ...
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bioRxiv - Cell Biology 2022Quote: ... Single cells were dispatched into a 96-plate well containing 5μL 1x lysis buffer containing Murine RNase inhibitor (NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina).
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bioRxiv - Immunology 2022Quote: ... 5,000-50.000 cells per experiment were centrifuged 5 minutes at 8°C, and resuspended in 25 µl transposase mixture (12.5 µl Tagmentation DNA buffer, 1 µl Tn5 transposase (Illumina), 10.75 µl nuclease-free water ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were removed and isolated nuclei were resuspended in 50 μl of transposition mixture (25 μl of TD buffer, 2.5 μl of Tn5 transposase (Illumina), and 22.5 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ul of Tn5 tagmentation mix (1X Tagment DNA buffer, Illumina; ATM mix, Illumina and cDNA library 1 ng) was prepared for cDNA fragmentation ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted to 650 pM in RSB buffer and mixed with 1% PhiX Control v3 DNA (Illumina FC-110-3001) for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Metagenomic shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina Inc., San Diego, CA, USA) and subject to paired-end sequencing (2 × 150 bp ...
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bioRxiv - Cell Biology 2020Quote: ... Poly(A) selected pair-end sequencing libraries were generated using the Illumina TrueSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were performed prior to cDNA library preparation using the Illumina TruSeq Stranded mRNA sample preparation kit (Illumina, CA, USA). Globin transcript depletion ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were extracted by resuspending the samples in 50 μl transposition reaction Tn5 mix (Illumina Nextera DNA Library Kit) (FC121-1030 ...
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bioRxiv - Genetics 2021Quote: Purified samples were then amplified by PCR (maximum 12 cycles) using barcoded primers from the Nextera Index Kit (Illumina). To determine the optimal number of cycles for each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were prepared from 1 μg total RNA using the TruSeq Standard mRNA LT Sample Preparation Kit (Illumina) and sequenced by Illumina NextSeq500 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: The libraries were prepared for 150bp paired-end sequencing using TruSeq stranded mRNA Sample Preparation Kit (Illumina, CA, USA). Namely ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... An RNA-Seq library was prepared using the Illumina TruSeq RNA sample preparation kit v.2 (Illumina, CA, USA) following Illumina’s Low-throughput protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... eurysternus samples (complete metadata including barcodes are available in Supplementary data 1. The purified library was sequenced on Illumina Miseq using V2 chemistry with 500 cycles (Norwegian High Throughput Sequencing Centre ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library was constructed using the TruSeq™ Nano DNA LT Sample Prep Kit–Set A (Illumina, USA) and amplified using the TruSeq PE Cluster Kit (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries for PE short reads were constructed using an Illumina TruSeq DNA LT Sample Prep Kit (Illumina, CA, USA). The PE library of WRC17 and other libraries were sequenced on the Illumina MiSeq and HiSeq 4000 platforms.
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bioRxiv - Neuroscience 2021Quote: ... samples were used for library construction with the TruSeq Stranded mRNA Library Prep Kit from Illumina (Cat. No. 20020595), as well as the KAPA Dual-Indexed Adapter Kit from Roche (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribosomal RNA was removed from the samples using Ribo-Zero Gold rRNA Removal Kit (Yeast) following manufacturer instructions (Illumina). RNA was quantified using a Quant-iT assay (Life Technology) ...
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bioRxiv - Molecular Biology 2021Quote: ... The V4 region was amplified using 0.5 ng of DNA extracted from F and LL and the universal primer pairs 515F and 806R (underlined nucleotides in the following sequences) designed to contain from 5′ to 3′ ends the transposon Nextera’s sequences (Nextera DNA sample preparation guide, Illumina): 515F ...
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Genome of Solanum pimpinellifolium provides insights into structural variants during tomato breedingbioRxiv - Genomics 2020Quote: ... an Illumina paired-end library with insert size of ~450 bp was constructed using the Illumina Genomic DNA Sample Preparation kit following the manufacturer’s instructions (Illumina), and a Hi-C library was prepared following the proximo Hi-C plant protocol (Phase Genomics) ...
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bioRxiv - Genomics 2020Quote: ... Samples that passed quality checks were tagmented with the Nextera DNA Library Prep Kit (Illumina cat# FC-121-1030). Tagmented samples were cleaned using the QIAquick DNA column (Qiagen cat# 28104) ...
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bioRxiv - Genomics 2020Quote: Sequencing libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit v2 (ref. RS-122-2101/2, Illumina) according to the manufacturer’s instructions (unless specified otherwise) ...
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bioRxiv - Genetics 2021Quote: ... Each sample was sequenced in multiple HiSeq2000 lanes using the NextSeq 500 v2 Cycle Kit (Illumina, San Diego, CA) in order to obtain 2 × 75-bp reads.
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bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced samples on an Illumina HiSeq-2500 1TB platform (101nt read length; Illumina, Inc., San Diego, CA, USA). Of the 199 strains submitted to JGI ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The libraries of the northern and southern sample were each sequenced on a separate lane of a HiseqX (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... Strand-specific 354mRNA sequencing was performed on total RNA and using TruSeq stranded mRNA sample prep 355kit LT (Illumina), as previously described72 ...