Labshake search
Citations for Illumina :
7501 - 7550 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and 8.8 µl of paired i7 and i5 Nextera XT Index Kit V2 indexing primers (Illumina, Cat. No. FC-131-2001) were added to each Tagmentation reaction ...
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bioRxiv - Microbiology 2021Quote: ... Nucleotide sequencing was performed using the TruSeq RNA sample preparation kit in an Illumina HiSeq 2500 instrument (Illumina, San Diego, USA) with a paired-end and 150-base-read protocol in RAPID module ...
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bioRxiv - Neuroscience 2020Quote: ... retinas of WT or Prom1-KO mice and were used to synthesise cDNA libraries with a TruSeq stranded-mRNA library preparation kit (Illumina, 20020594). The libraries were sequenced with the NextSeq 500 platform (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two DNA pool-seq libraries were prepared according to manufacturer’s protocols using the Illumina TruSeq Nano DNA HT Library Prep Kit (Illumina, California, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... Extracted nuclei was processed for TN-5 mediated tagmentation using the Illumina Tagment DNA Enzyme and buffer kit (Nextera Illumina # 20034210) ...
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bioRxiv - Neuroscience 2021Quote: ... Tagmentation by Tn5 was performed using reagents from the Nextera DNA Sample Preparation Kit (FC-121-1030, Illumina; San Diego, CA) as previously described [5] ...
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bioRxiv - Cancer Biology 2021Quote: ... above 9.0 and normalized to 500 ng total RNA for library preparation using TruSeq® stranded mRNA sample prep kit (Illumina, #20020595). After library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA sequencing libraries were generated using the TruSeq Stranded RNA Library Prep Kit v2 (RS-122- 2001, Illumina, San Diego, CA) and 100 bp paired-end reads were sequenced on an Illumina HiSeq 2500 to at least 26 million reads per sample at the Functional Genomics Core Facility at UCSF ...
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bioRxiv - Immunology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... libraries for sequencing were prepared from using the TrueSeq RNA Sample Prep Kit v2-Set B (Illumina, San Diego, CA, US) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Ribosomal RNAs were depleted from 5 μg of total RNA of the sample by Ribo-Zero rRNA Removal Kit (Seed, Root) (Illumina®). After rRNA depletion ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.1 micrograms of extracted DNA was utilized as starting material for TruSeq DNA PCR-Free library preparation kit (Illumina Inc., USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-Seq libraries were prepared using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (RS-122-2201, Illumina Inc.) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sequencing was performed on an Illumina MiSeq platform using Reagent Kit v2 [2 x 300 cycles] (Illumina Inc., CA, US). Demultiplexing and trimming of Illumina adapters and barcodes was done with Illumina MiSeq Reporter software (version 2.5.1.3).
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bioRxiv - Genomics 2020Quote: ... and sequenced on an Illumina NextSeq 500 (NextSeq control software v2.0.2/Real Time Analysis v2.4.11) using a 150-cycle NextSeq 500/550 High Output Reagent Kit v2 (Illumina, FC-404-2002) in standalone mode as follows ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing of the pooled libraries was performed using an Illumina MiSeq benchtop sequencer and 2×250bp paired-end reads MiSeq v2 kit-500 cycles reagents (Illumina, USA). Raw reads were trimmed of remnant sequencing adapters sequences and low-quality regions (Phred score < Q20 ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing of the resulting library was carried out from both ends (2×300 bp) with the 600-cycle MiSeq Reagent Kit v3 on MiSeq (Illumina, USA) and the adapters trimmed from the resulting reads at the facility.
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bioRxiv - Immunology 2020Quote: ... dilution with TruSeq® Stranded mRNA Library Prep for NeoPrepTM kit as per the NeoPrep Library Preparation System Guide on the instrument (Illumina). The libraries were diluted 1:5 (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... Ethanol precipitated samples were resuspended in FPF sample buffer from the TruSeq stranded mRNA-Seq kit reagents (Illumina, San Diego, CA). mRNA abundance was quantified using a Quant-iT RNA Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... 16S rRNA gene sequencing was performed on normalized pooled amplicons using the Illumina MiSeq System using a MiSeq reagent kit v2 (300 cycles; Cat. No. MS-102-2002, Illumina Inc.). FASTQ files for reads 1 (forward) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic libraries were constructed form the DNA samples using the Nextera XT DNA Sample Preparation Kit (Illumina; San Diego, CA, USA), and paired-end sequencing (2×250 bp ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Microbiology 2021Quote: ... at the Centro de Biotecnología Vegetal, Universidad Andrés Bello (Santiago, Chile) using the TruSeq Nano DNA Kit (Illumina Inc., CA, USA.) following the TruSeq Nano DNA Sample Preparation Guide 15041110 Rev ...
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bioRxiv - Immunology 2021Quote: ... Final libraries were pooled and sequenced using a High output single-end 75 cycle (version 2) sequencing kit using the Illumina Nextseq 550 platform (Illumina, USA).
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bioRxiv - Immunology 2021Quote: ... single reads and 7 bases index read on an Illumina HiSeq2000 instrument using the TruSeq SBS Kit HS-v3 (50-cycle, FC-401-3002, Illumina Inc). RNA seq data in FASTQ form from each sample were first quality assessed using fastQC ...
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bioRxiv - Cell Biology 2021Quote: cDNAs were prepared from lysed cells according to SMART-Seq2.0 (Picelli et al., 2014) for low input RNA sequencing and libraries were prepared with Nextera® DNA Library kit (Illumina). Independent biological replicates of each cell type sequenced using Illumina HiSeq2500 and obtained ∼20 million 75 bp single-end reads (7 replicates for β-cells ...
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bioRxiv - Genomics 2021Quote: PCR-free Illumina libraries were generated from 1 μg genomic DNA using a Covaris LE220-plus to shear the DNA and the TruSeq® DNA PCR-Free HT Sample Preparation Kit (Illumina) for library generation ...
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bioRxiv - Genomics 2021Quote: ... short-inserts of 150-bp paired-end libraries were prepared for each individual using a Nextera XT DNA Library Preparation Kit (Illumina ®). Size-selection and cleanup were accomplished using CA-magnetic beads (Dynabeads® MyOne Carboxylic Acid ...
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bioRxiv - Genomics 2021Quote: ... 10 nM of the cDNA library from every single cell was treated with the NovaSeq 6000 S4 reagent kit and sequenced by Nova-Seq 6000 (151 bp, paired-end) (Illumina KK).
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold kit (Illumina, San Diego, CA) and sequenced on Illumina NextSeq 500 sequencer with 75-nucleotide single-end reads to reach average 32 million reads per sample ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in 50 ul of the transposase reaction buffer (Nextera DNA library prep kit (Illumina cat. FC-121- 1030) and 22.5 ul nuclease-free water ...
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bioRxiv - Genomics 2021Quote: For miRNA expression analysis 2 µg of the small RNA was polyadenylated using the Poly(A) Tailing kit (epicenter-An Illumina company) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were prepared with 800 ng of total RNA by using TruSeq Stranded Total RNA Ribo-Zero Gold Prep kit (Illumina, #20020598) allowing depletion of cytoplasmic and mitochondrial rRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The pooled libraries were sequenced on the Illumina NextSeq 550 platform using the pair-ended 75 bp by a 150-cycle High Output v2.5 Kit (#20024907, Illumina, Inc., USA). We used ultrafast universal RNA-seq aligner STAR (v2.5.3a ...
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bioRxiv - Immunology 2022Quote: ... value of the samples used for library preparation was ≥8 and cDNA libraries were prepared using IlluminaTruSeq Stranded mRNA Sample Prep kit (Illumina, USA) from 4μg of total RNA according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... The total RNA was transformed into a library of template molecules using TruSeq® Stranded mRNA Sample Preparation Kits (Illumina®) and the EpMotion 5075 (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... Two indexed libraries were equimolarly pooled and sequenced on Illumina NOVAseq 6000 platform using the v2 Kit (Illumina, San Diego, CA) with a customized paired-end ...
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bioRxiv - Microbiology 2022Quote: ... 500 ng of total RNA with an RNA Integrity Number (RIN) greater than 9.5 were used to prepare sequencing libraries with the TruSeq® Stranded mRNA Library Prep Kit (Illumina: 20020594). Library concentrations were measured with Qubit dsDNA HS Assay Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina RS-122-2001) using Superscript III for reverse transcription instead of Superscript II (incubation time ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was then mixed and incubated at 37°C for 1h min with the transposition reaction mix prepared using Illumina Tagment DNA Enzyme and Buffer kits (Illumina, 20034197). DNA was then purified using DNA Clean and Concentrator kit (Zymo ...
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bioRxiv - Bioengineering 2022Quote: ... 200 ng of total RNA was used to make RNA sequencing libraries using the TruSeq Stranded mRNA Prep Kit (Illumina 20020595). Libraries were run on a 51-cycle NovaSeq (SP flowcell ...
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bioRxiv - Cell Biology 2022Quote: ... Transcriptome sequencing was then performed using an Illumina NextSeq 500 following library preparation with a Stranded mRNA Library Prep kit (Illumina, #20020594) with TruSeq RNA Single Indexes Set A (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the cells were precipitated and kept on ice and subsequently resuspended in 25μl 2X Tagment DNA Buffer (Illumina Nextera kit), 2.5μl Transposase enzyme (Illumina Nextera kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The ribo-zero treated RNA was used to create multiplexed RNA-Seq libraries using the NEBNext Ultra RNA Library Prep Kit (Illumina E7530) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were generated at the Huntsman Cancer Institute Genomic Core facility using TruSeq Stranded Total RNA Library Prep with Ribo-Zero Gold kits (Illumina 20020598) and sequenced using the Illumina NovaSeq 6000 for 100M 2×50bp reads ...
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bioRxiv - Genomics 2022Quote: ... RNA samples >9 in RIN numbers were selected for high-quality libraries preparation using TrueSeq Stranded mRNA LT Sample Prep Kit and sequenced in HiSeq 4000 (Illumina, USA).
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bioRxiv - Genomics 2022Quote: ... These products were then used for index PCR using the SI-PCR Primer from the 10X kit for the i5 and one of the small RNA TrueSeq index primers for the i7 (Illumina #15004197).
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bioRxiv - Genomics 2022Quote: ... The size of Nextera libraries after being purified with AMPure XP Beads was determined using a 4200 TapeStation System and quantified with Qubit dsDNA HS Assay.1.8 pM of pooled libraries was sequenced on Illumina NextSeq 550 sequencer using Nextseq 550 High Output v2 kit (75 cycles) (Illumina, #20024906) in paired-end mode ...
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bioRxiv - Genomics 2022Quote: ... Whole genome bisulfite sequencing libraries were prepared (according to Accel-NGS Methyl-Seq DNA library Kit for Illumina protocol, Swift Biosciences) and sequenced in 150 bp paired-end reads by Novogene company.