Labshake search
Citations for Illumina :
7451 - 7500 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... One microgram of total RNA was used to prepare libraries for sequencing using an Illumina TruSeq Stranded mRNA HT kit (Illumina, 20020594) and SuperScript2 Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Short reads were obtained using NovaSeq6000 S2 Reagent Kit (300cycles) and S2 flow cell on a NovaSeq6000 (2×150-bp paired-end) system (Illumina, USA) at Eurofins Genomics GmbH ...
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bioRxiv - Genomics 2020Quote: Paired-end sequencing was performed on a NextSeq 500 instrument using a high output v2 kit (Illumina, San Diego, CA, USA) with a read length of 75 nucleotides to an average sequencing depth of 11 million read pairs in the discovery cohort ...
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bioRxiv - Microbiology 2021Quote: Short-read paired-end sequencing of DNA and rRNA-depleted samples was performed on the Illumina Novaseq platform (S prime, 2x 150 bp) using TruSeq PCR free DNA library preparation kit (Illumina Inc.).
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bioRxiv - Microbiology 2021Quote: Purified PCR products were given unique dual indexes at the 5’ end using the Nextera XT Index Kit v2 index primers (Illumina, USA). To attach the index primers ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were constructed from 100-1,000 ng of total RNA using the TruSeq total stranded mRNA Library Prep Kit (Illumina, San Diego). Library concentrations were assessed using a Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Barcoded stranded mRNA-seq libraries were prepared using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...
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bioRxiv - Microbiology 2021Quote: ... All libraries were then pooled in a concentration at 4nM and sequenced on a NextSeq 500 Illumina sequencing platform system using NextSeq 500/550 High Output Kit v2.0 (150 cycles) (Illumina, Inc. USA).
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bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted and RNA-seq libraries were prepared using the TruSeq RNA sample prep kit v2 (Illumina, San Diego, CA). 75-bp reads were obtained using an Illumina NextSeq500 sequencer at Northwestern Sequencing Core (NUSeq Core) ...
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bioRxiv - Pathology 2022Quote: Two hundred fifty ng of total RNA from each of the brain samples was used for library preparation with the Illumina TruSeq RNA kit (Illumina, USA) by a half-scale method of the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Amplified cDNA (0.125-0.375 ng) was used for generating sequencing libraries with the Illumina Nextera XT DNA kit (Illumina, FC-131-1096). All libraries were sequenced as paired-end 75 using the Illumina HiSeq4000 system.
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bioRxiv - Immunology 2022Quote: ... The remaining lysis supernatant containing the nuclei were immediately placed into a transposase reaction using the Tagment DNA TDE1 enzyme kit (Illumina 20034197) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA libraries were generated using the same amount of RNA for each sample according to the Illumina TruSeq Stranded Total RNA kit with an initial ribosomal depletion step using Ribo Zero Gold (Illumina, CA). The libraries were quantified by qPCR and sequenced in paired-end mode (2×75 bp ...
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bioRxiv - Genomics 2022Quote: ... The normalized libraries were sequenced for 150 bp paired-end reads on Illumina NextSeq 500 platform using NextSeq 500/550 v2 and v2.5 sequencing reagent kits (Illumina Inc., USA) at NGS facility ...
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bioRxiv - Systems Biology 2020Quote: ... We sequenced all RNAtag-seq libraries in batches of 96 samples on an Illumina NextSeq 550 using 75 cycle high-output kits (Illumina 20024906).
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bioRxiv - Immunology 2020Quote: ... Supernatant was discarded and nuclei were re-suspended in 50 μl reaction buffer containing 5.0 μl Tn5 transposase and 10 μl of 5 × TTBL buffer (TruePrepTM DNA Library Prep Kit V2 for Illumina, Vazyme Biotech). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 50ng of each rRNA-depleted samples were then used for construct RNA-seq libraries using the ScriptSeq v2 RNASeq Library Preparation Kit (Illumina SSV21124). Libraries were sequenced on an Illumina PE151 platform.
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bioRxiv - Evolutionary Biology 2020Quote: ... 500 ng of the purified and pooled PCR genome amplicons were prepared for sequencing via Nextera DNA Flex Kit (Illumina, #20018705) with 96 indexes (Illumina ...
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bioRxiv - Genetics 2020Quote: ... Exome sequencing was performed using NextSeq 500/550 High output kits v2 (150 cycles) on Illumina NextSeq 550 system (Illumina, USA) with the coverage of 100x
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bioRxiv - Molecular Biology 2020Quote: We performed 8 additional cycles of PCR with Nextera 24-Index kit for indexing before sample pooling (Illumina, FC-121-1011), for which we used 7.5 μl of the above elute as template ...
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bioRxiv - Microbiology 2021Quote: ... All amplicon libraries were then paired-end sequenced (300 bp reads) on an Illumina MiSeq with a v3 600 cycles kit (Illumina, USA).
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bioRxiv - Physiology 2020Quote: ... Samples were sequenced Single-reads 76 bases using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005), and primary data analysis was performed with the Illumina RTA version 2.4.11 and Basecalling Version bcl2fastq-2.20.0.422.
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bioRxiv - Plant Biology 2020Quote: ... 34 µL DNase-free water was added to 16 µL precipitated DNA and adaptors were ligated to DNA fragments according to the manufacturer’s instructions (NEBNext Multiplex Oligos for Illumina kit NEB). After ligation ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were constructed for performing whole-transcriptome sequencing using the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA) and sequenced using the 100-bp paired-end mode of the TruSeq Rapid PE Cluster Kit and the TruSeq Rapid SBS Kit (Illumina).
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bioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were prepared with 1 µg of total RNA for each sample using the Illumina TruSeq mRNA kit (Illumina, USA) as recommended by the manufacturer using the Illumina CD RNA indexes (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting RNA was used to prepare an RNA-seq library using NEBNext Ultra™ Directional RNA Library Prep Kit from Illumina, according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... aeruginosa (Pae5 and Pae1505) genomic DNA sequencing libraries were prepared using Nextera DNA library prep kit (Illumina, Cat # FC-121-1031) and utilizing the Nextera DNA Sample Preparation Index Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing of the amplicon library was performed on the Illumina MiSeq System with MiSeq reagent kit v2 (Illumina Inc., CA, USA), including 5.0% PhiX as an internal control.
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bioRxiv - Microbiology 2020Quote: ... The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using mid output 150-bp paired-end (PE) sequencing kit (Illumina, 20024905).
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bioRxiv - Microbiology 2020Quote: ... Experiments were conducted in triplicate and samples sent to Macrogen (Seoul, South Korea) for Ribosomal RNA reduction using Ribo-Zero™□ rRNA Removal kit (Illumina), library preparation using the TruSeqⓇ stranded mRNA kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were prepared using TruSeq stranded Total RNA sample preparation kit with Ribo-Zero according to the manufacturer’s protocol and sequenced using HiSeq 2500 (Illumina, CA, USA), generating 125-bp paired-end reads (see the RNA-seq analysis workflow in Fig 7).
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bioRxiv - Molecular Biology 2020Quote: Ribosomal RNA was depleted from the total RNA using the Ribo-Zero Gold rRNA removal kit (Human/Mouse/Rat) (Illumina, USA). The ribo-depleted RNA was used to create an ssRNA-Seq library ...
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bioRxiv - Microbiology 2021Quote: ... and sequenced under a 150-bp read length using Illumina HiSeq 4000 systems and HiSeq SBS Kit v4 reagents (Illumina, USA). Read demultiplexing and adapter trimming were performed by the Imperial BRC Genomics Facility using their production pipeline.
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bioRxiv - Systems Biology 2020Quote: ... Final loading concentration of 1.8 pM and 1% PhiX (v/v) was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina). Samples were sequenced paired-end with 75 cycles and single index of 6 cycles.
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bioRxiv - Cancer Biology 2020Quote: ... cell nuclei were extracted using cold lysis buffer (see supplementary methods) and submitted to tagmentation (Nextera DNA Library Preparation Kit, Illumina, USA). After purification ...
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bioRxiv - Genomics 2021Quote: ... from 2 days differentiated TX1072 mESCs was subjected to strand-specific RNA-seq library preparation with the TruSeq® RNA Sample Preparation Kit v2 (Illumina), which included polyadenylated RNA enrichment using oligo-dT magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... The Illumina MiSeq paired-end sequencing service was provided by the Genomics Core (Institute of Molecular Biology, Academia Sinica) using the MiSeq Reagent Nano Kit v2 (MS-103-1003; Illumina, USA). For the reference isolate GV230 ...
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bioRxiv - Genomics 2021Quote: ... Samples were concentrated using a centrifugal evaporator Speed Vac® to a final volume of 5 μl and we started the TruSeq Small RNA Sample Preparation Kit (Illumina) protocol according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Custom RNA capture-based libraries were prepared starting from 8.5 μL eluate for biofluid samples and 100 ng RNA for FFPE and MAQCA/B using the TruSeq RNA Exome Library Prep Kit (Illumina, USA). Library preparation happened according to the manufacturer’s protocol with some minor modifications ...
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bioRxiv - Genomics 2021Quote: ... Approximately 1000 ng of extracted RNA was used to prepare libraries for sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina, 20020594). Libraries were sent for paired-end sequencing on the NovaSeq 6000 (100 bp paired-end reads) ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was done with the Nextera DNA Flex Library Prep Kit following manufacturers’ protocol (Illumina Inc. San Diego, CA, USA) and quality was checked using the Agilent Bioanalyzer 2100 High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Genetics 2020Quote: ... A total of 2 μg RNA was used for mRNA isolated and RNA-seq library construction using the TruSeq RNA Library Preparation Kit (Illumina, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... parental DNA was extracted from one individual male and female from tail-fin clip and sequencing library were synthesized with Nextera XT Kit (Illumina, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... we used the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina paired-end sequencing library (Illumina, San Diego, CA, USA) and the xGen hybridization capture system with Exome Research Panel v2 (Integrated DNA Technologies ...
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bioRxiv - Genetics 2020Quote: ... The Illumina MiSeq platform was used with the shotgun 2 x 250 paired-end sequencing methodology (Nextera DNA Preparation Kit, Illumina, USA). The sample used 0.33 of a flow cell and primer design was optimized for use with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Sequencing libraries were prepared from 100 ng DNA using the TruSeq Nano DNA sample preparation kit (cat# 20015964/5, Illumina Inc.) targeting an insert size of 350bp ...
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bioRxiv - Cell Biology 2020Quote: ... Samples with RNA Integrity Number (RIN) >8 were subjected TruSeq Poly-A mRNA Library Pro Kit protocol 15031047 RevD (Illumina, USA) to generate indexed cDNA libraries ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA libraries for sequencing were constructed using Nextera XT DNA Library Prep kit (FC-131-1024, Illumina, San Diego, CA). The 24 prepared libraries were pooled and submitted to Michigan State University Genomics Facility ...
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bioRxiv - Genomics 2021Quote: ... and paired-end sequenced on Illumina HiSeq 2500 platform using TruSeq SBS Kit v3-HS (200 cycle) (Illumina, San Diego, CA) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared based on a 20X scaled-down Nextera XT DNA Library Prep Kit (Illumina, Cat. No. FC-131-1024) protocol (35 ...