Labshake search
Citations for Illumina :
7351 - 7400 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Tagmentation with Tn5 transposase was performed on 90 ng aliquots of the cDNA library using an Illumina DNA Prep kit (Illumina, 20018704) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... A cDNA library was constructed for each sample using a TruSeq Stranded mRNA library Prep kit (Illumina, Cat# RS-122-2101) following manufacturer’s instructions ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... 0.5-1 μg of RNA were used to prepare libraries for RNA-seq with the TruSeq stranded total RNA Sample Prep Kit (Illumina, #20020596) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole genome bisulfite sequencing (WGBS) libraries were generated from the converted samples with the TruSeq DNA Methylation kit (Illumina Cat#EGMK81312). Consistent fragment size distribution of the resulting libraries was assessed via micro-capillary gel electrophoresis on a Bioanalyzer 2100 (Agilent Cat#G2939BA) ...
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bioRxiv - Molecular Biology 2020Quote: Deep sequencing was carried out on an Illumina NextSeq using commercially available kits from Illumina (Danyel Biotech, Cat #FC-404-2005) following the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2020Quote: ... Illumina sequencing libraries were prepared according to the protocol in the Nextera XT Library Sample Preparation kit (Illumina, FC-131-1096). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... using 2 x 100bp paired-end reads and 2 x 8bp or 2 x 12bp index reads with a 300-cycle kit (Illumina 20012860).
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bioRxiv - Cell Biology 2022Quote: ... For library preparation an amount of 2 mg of total RNA per sample was processed using Illumina’s TruSeq RNA Sample Prep Kit (Illumina, CA, USA) following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the library was sequenced on Illumina Hiseq platform with cBot System via TruSeq PE (Paired-End) Cluster Kit v3-cBot-HS (Illumina, US). For WGBS data analysis ...
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bioRxiv - Genomics 2020Quote: ... and paired-end sequenced on Illumina HiSeq 2500 platform using TruSeq SBS Kit v3-HS (200 cycle) (Illumina, San Diego, CA) following the manufacturer’s recommendations.
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bioRxiv - Genomics 2020Quote: ... The transcriptomic library was prepared with TruSeq Stranded Total RNA Library Preparation kit by following the manufacturer’s protocol with Ribo-Zero Workflow (Illumina, Inc., USA). The quality of libraries was evaluated on Agilent 2200 TapeStation using High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Genomics 2020Quote: ... 25 μL TD buffer and 2.5 μL TDE1 enzyme from Nextera DNA Library Prep Kit (Illumina, cat. no. FC-121-1030)) ...
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bioRxiv - Genomics 2020Quote: ... quantitated by real-time quantitative PCR (qPCR) using a TaqMan® Probe and amplified off-board on a cBot (TruSeq PE Cluster Kit v3–cBot–HS, Illumina) to generate clusters on the sequencing flow cell ...
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bioRxiv - Genomics 2020Quote: The immunoprecipitated sample and the input were subjected to Nextera library preparation as described by the manufacturer (NEBNext Ultra II DNA library Prep Kit for Illumina, NEB). The quality and size of libraries and chromatin shearing fragments were checked by Agilent Bioanalyzer using Agilent DNA High Sensitivity Chips (Agilent Technologies) ...
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bioRxiv - Immunology 2021Quote: ... One µg of total RNA was used to construct small RNA libraries using the TruSeq Small RNA Sample Prep Kit (Illumina, USA). The resulting library was sequenced with the single-end sequencing (36 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA-seq libraries were prepared from 50ng of rRNA-depleted RNA using the TruSeq® Stranded Total RNA Sample Preparation Kit (Illumina). Paired-end sequencing (2×50 nt ...
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bioRxiv - Physiology 2020Quote: ... All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina, San Diego, California, USA), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized using the MMLV High Performance Reverse Transcriptase kit according to the instructions of the supplier (epicenter, an Illumina company).
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA LP Sample Prep Kit (Illumina, Cat. No. 20020594) with TruSeq RNA Single Indexes Set A and B (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... the sequencing libraries were constructed using TruSeq Stranded Total RNA with the Ribo-Zero Plant kit (Illumina, San Diego, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... sequencing libraries were generated from whole blood-derived fragmented DNA using the TruSeq DNA Nano kit (Illumina, Inc., San Diego, USA) and sequence reads were generated using a HiSeq X Ten1 system (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The final pool was sequenced on Illumina MiSeq platform 2×300 bp using the MiSeq Reagent Kit V3 (600 cycles PE) cartridge (Illumina, USA).
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bioRxiv - Cancer Biology 2020Quote: ... TN5 tagmentation and library amplification realized 3’end fragments by Nextera XT DNA Sample Preparation Kit (Illumina, Cat#FC-131-1024) according to the manufacturer’s instructions while P5_TSO and Nextera_N7xx took the place of the custom primers ...
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bioRxiv - Immunology 2021Quote: ... 1.5 ng cDNA was tagmented using 0.5 μl TruePrep Tagment Enzyme V50 and 1x TruePrep Tagment Buffer L (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme), followed by an incubation step at 55 °C for 10 min ...
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bioRxiv - Genomics 2020Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... An aliquot of 500 ng of RNA was used to prepare a polyadenylated RNA library with the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Sequence libraries for PE short reads were constructed using an Illumina TruSeq DNA LT Sample Prep Kit (Illumina K.K., Tokyo, Japan). The libraries were sequenced on a HiSeq high output (150 bp ...
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bioRxiv - Microbiology 2021Quote: ... 16S amplicon libraries were prepared by addition of Nextera XT Index Kit v2 Set A adapter sequences (Illumina, San Diego, CA) which were cleaned using KAPA Pure Beads (Roche ...
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Transcriptomic Profiling of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Over TimebioRxiv - Cell Biology 2020Quote: ... Libraries were sequenced on an Illumina NextSeq 500 (NextSeq Control Software v2.2.0 / Real Time Analysis v2.4.11) using NextSeq 500/550 High Output Kit v2.5 (150 Cycles) (20024907, Illumina, San Diego, CA, USA) as follows ...
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bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina, USA). Sequencing was performed on the Illumina NovaSeq 6000 (CNS-RNA-seq cohort subset ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sequencing libraries were prepared using the Nextera DNA Flex library kit according to the manufacturer’s instructions (Illumina Ltd., Cambridge, UK). Libraries were sequenced on a MiSeq (Illumina ...
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bioRxiv - Bioengineering 2020Quote: ... rRNA was removed from 100 ng of total RNA using Ribo-Zero(TM) rRNA Removal Kit (Illumina Biotechnology, San Diego, CA). Stranded cDNA libraries were generated using the Illumina Truseq Stranded mRNA Library Prep kit ...
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bioRxiv - Biochemistry 2020Quote: ... Sheared DNA was used to construct the library using the Illumina TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, 20015962). After adapter ligation ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries from knockout FL83B cells and their corresponding non-targeting controls were prepared with TruSeq Stranded mRNA Library Kit (Illumina, #20020595), pair-end sequencing was performed using the NovaSeq 6000 (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... and paired end read sequencing (2 x150bp) was performed using MiSeq platform and reagent kit v2 (Illumina, San Diego, CA, USA). For sequences obtained by HTS a pre-alignment quality check was performed using the FASTQC program v0.11.8 and the Trim Galore script (52 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg of high-quality total RNA (RIN > 9.9) was processed with Truseq Stranded Total RNA Library Prep Kit (Illumina; Cat#: 20020596) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... cDNA was subsequently tagmented and amplified for 12 cycles by using a Nextera XT DNA Library Preparation Kit (Illumina FC-131). Sequencing libraries were analyzed with Qubit and Agilent Bioanalyzer ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
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bioRxiv - Plant Biology 2020Quote: ... The gDNA was isolated by using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Sequencing was performed on an Illumina MiSeq platform at the University of Manchester genomics facility using shotgun 2×250 paired-end sequencing methodology (Nextera DNA Preparation Kit, Illumina, USA). The sample used 0.33 of a flow cell and primer design was optimized for use with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... The sequencing step of PCR fragments was done on an Illumina Miseq personal sequencer using the MiSeq Reagent Kit v3 (Illumina®) followed by quality control processes for libraries using the PippinHT system from SAGE Sciences for libraries purification ...
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bioRxiv - Plant Biology 2021Quote: ... Two 150-bp single-end sequencing libraries were prepared using the NextSeq 500/550 High Output Kit (Illumina, San Diego, USA) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Metatranscriptomic sequencing libraries were also constructed by Genome Quebec using an Illumina TruSeq RNA Library Preparation kit (Illumina, Inc., CA, US) after rRNA depletion ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... The strand specific RNAseq library was prepared using the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Palo Alto, CA, USA) at the Oxford Genomics Center ...
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bioRxiv - Microbiology 2021Quote: ... A total amount of 100 ng total RNA per sample was used to prepare for the rRNA-depleted cDNA library by Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit (Illumina, USA). The final library size and quality was evaluated using an Agilent High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... DNase-treated RNA extracts were used for library preparation using the TruSeq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Transposase Enabled (LITE) Illumina compatible libraries were constructed using a modified protocol based on the Illumina Nextera kit (Illumina, California USA). A total of 1ng of DNA was combined with 0.9 μl of Nextera reaction buffer and 0.1 μl Nextera enzyme in a reaction volume of 5 μl and incubated for 10 minutes at 55°C ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... A total of 100 ng of RNA input was then used for cDNA library preparation using the TruSeq® Stranded mRNA NeoPrep kit (Illumina), according to manufacturer instructions ...
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bioRxiv - Pathology 2021Quote: Samples were subjected to mRNA enrichment through eukaryote and prokaryote rRNA removal using the Ribo-Zero rRNA Removal Epidemiology Kit (Illumina®), following the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was isolated and used to prepare ChIP-seq libraries with the TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012). Sequencing was performed on an Illumina NextSeq500 in Georgia Genomics and Bioinformatics Core (GGBC ...