Labshake search
Citations for Illumina :
7251 - 7300 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... was used for RNA extraction and the TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat Kit (Illumina, 20020596) for library preparation ...
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bioRxiv - Plant Biology 2024Quote: ... The library constructions were generated using TruSeq Stranded mRNA Library Prep Kit (RS-122-2101; Illumina, San Diego, CA USA) and assessed to Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips for qualification ...
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bioRxiv - Molecular Biology 2024Quote: ... The index-coded samples were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer ‘ s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... the Mount Sinai Oncological Sciences Sequencing Core Facility prepared poly-A-capture RNA-seq libraries using the TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA recovered from the sorted droplets was amplified using Ampli-Barcode and NGS-Reverse primers (Supplementary Table 2) to append P5 and P7 sequences subsequently used for molecular indexing using the Nextera Indexing kit (Illumina) as recommended by the supplier ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase (Illumina), as well as 0.01% digitonin ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries from mice of the same age were then pooled and each loaded at 2 nM on 4 lanes for clustering on a paired-end Illumina Flow Cell using the HiSeq 4000 PE Cluster Kit (ref. PE-410-1001; Illumina) and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq 4000 SBS Kit (ref ...
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bioRxiv - Neuroscience 2024Quote: ... Samples (with RNA integrity number >8) were used for library generation using the TrueSeq RNA sample Prep Kit v2 (Illumina).
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bioRxiv - Microbiology 2024Quote: ... and sequencing was carried out at the Bio-Environment platform (University of Perpignan Via Domitia) using the Nextera XT DNA Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The quantified RNA was subsequently used for library construction using the NEBNext Ultra Directional RNA Library Prep Kit and sequenced on the NovaSeq 6000 platform (PE150, Illumina), yielding approximately 19.76 Gb of paired-end reads generated per sample ...
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bioRxiv - Cell Biology 2024Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using SR Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and mate-pair libraries of 3-kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We performed DNA shearing for the fragment and mate-pair library preparations using Covaris S220 ...
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bioRxiv - Genetics 2024Quote: ... Pools were sequenced with 75 cycle run kits (26bp Read1, 8bp Index1 and 55bp Read2) on the Novaseq Sequencing System (Illumina) to 80-90% saturation level.
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bioRxiv - Neuroscience 2024Quote: ... 500 ng of total RNAs were used to prepare sequencing libraries using the TruSeq Stranded total RNAs Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA was quantified using the Agilent 2100 bioanalyzer and applied to construct sequencing libraries using the TruSeq Stranded Total RNA Library Prep Kit (Illumina). The generated DNA libraries were sequenced at the Center for Cancer Research Sequencing Facility ...
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bioRxiv - Genomics 2024Quote: ... cDNA was prepared using a modified protocol based on the Illumina Truseq RNA Sample Preparation Kit (Illumina, San Diego, CA) and the published methods for strand-specific RNA-Seq [66 ...
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bioRxiv - Neuroscience 2024Quote: ... and then pooled in equimolar ratios for shallow sequencing on an Illumina MiSeq Reagent Kit v3 (Illumina, MS-102-3001) to determine cell capture rate ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng of the H2AK119ub1 ChIPed DNA and inputs were used for library preparation with the TruSeq ChIP Sample Preparation Kit (Illumina), and samples were sequenced as 150 bp paired-end reads as described above.
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bioRxiv - Genomics 2024Quote: ... The libraries were pooled equimolarly and sequenced on the Illumina NovaSeq 6000 using a NovaSeq Reagent Kit for 300 cycles (Illumina) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and sequenced on the Illumina NovaSeq 6000 sequencer with the SP-200 cycle reagent kit in order to generate approximately 50 million 100-base read pairs per sample (Illumina).The raw RNA-seq reads were first assessed for quality using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) ...
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bioRxiv - Zoology 2024Quote: ... A total of 24 sequencing libraries from 8 samples were generated using the TruseqTM RNA sample prep kit (Illumina, USA). First ...
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bioRxiv - Immunology 2024Quote: ... RNA was processed by the UCSD IGM genomics center to generate an RNAseq library using a TruSeq RNA Library Prep Kit v2 (Illumina). Raw sequencing data were aligned to mouse genome mm10 (Genome Reference Consortium GRCm38 ...
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bioRxiv - Genomics 2024Quote: ... To deplete the host rRNA we used the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, San Diego, CA, USA). The kit employs capture oligonucleotides and hybridization techniques to eliminate rRNA ...
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bioRxiv - Immunology 2024Quote: ... and library preparation was performed according to the TruSeq Stranded mRNA Sample Preparation protocol using the TruSeq Stranded mRNA HT Kit (Illumina). Sequencing was performed using an Illumina NovaSeq 6000 with a 2 50-bp read length at the Finnish Functional Genomics Centre at the University of Turku and Åbo Akademi as well as Biocenter Finland.
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bioRxiv - Genomics 2024Quote: ... The RNA-seq libraries were created according to the manufacturer’s instructions using the Tru-Seq RNA sample prep kit (Illumina, Inc.). In brief ...
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bioRxiv - Genomics 2024Quote: ... Sample replicates were subjected to library preparation with 12 cycles of amplification (NEBNext Ultra II DNA Library Prep Kit, Illumina). Libraries were sequenced on Illumina HiSeq (50 cycles ...
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bioRxiv - Immunology 2024Quote: ... Pooled batches were sequenced with 100 cycle run kits (28 bp Read1, 8 bp Index1 and 91 bp Read2) on the NovaSeq6000 Sequencing System (Illumina). Amplified cDNA was evaluated with an Agilent BioAnalyzer 2100 using a High Sensitivity DNA Kit (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... and subsequently processed by the UCSD IGM Genomics center to generate an mRNA-seq library using a TruSeq RNA Library Prep Kit v2 (Illumina). The library for Lin-c-Kitint/loFlt3+ progenitors was sequenced using Hiseq 4000 for single-end 100bp sequencing ...
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bioRxiv - Immunology 2024Quote: ... Amplified libraries were purified using a Qiagen PCR cleanup kit and sequenced for 50 cycles (paired-end reads) on a NovaSeq 6000 (Illumina). ATAC-seq was done in three biological replicates per genotype ...
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bioRxiv - Immunology 2024Quote: ... The library preparation was performed by The Australian Genome Research Facility (Melbourne, Australia) using the Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina). Libraries were then sequenced using the NovaSeq 6000 for 150-bp paired reads ...
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bioRxiv - Neuroscience 2024Quote: ... The 10x Genomics Chromium Single Cell 3’ Library and Gel Bead Kit v3 were used to construct scRNA-seq libraries and sequencing was conducted on the NovaSeq 6000 instrument (Illumina). Raw scRNA-seq data have been deposited within the Genome Sequence Archive for Human (https://ngdc.cncb.ac.cn/gsa-human/ ...
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bioRxiv - Genomics 2024Quote: ... Fully amplified libraries were purified using NEB Monarch PCR & DNA cleanup kits (Cat # T1030L, quantified by Qubit, pooled in an equimolar fashion, and submitted for paired-end sequencing on an Illumina NextSeq 500 at the Center for Genome Innovation (UConn ...
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bioRxiv - Systems Biology 2024Quote: ... and libraries were prepared using Nextetera XT DNA preparation kit (cat. no. FC-131-1024; Illumina, Inc., San Diego, CA), followed by multiplexed paired-end sequencing (read length of 150 bp) ...
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bioRxiv - Immunology 2024Quote: ... All captured samples and libraries were sequenced in one flow cell using a 100 cycle kit (paired-end) on a NovaSeq 6000 S4 (Illumina) targeting 20,000 read pairs/cell for gene expression ...
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bioRxiv - Immunology 2024Quote: ... Final libraries were sequenced using the NovaSeq 6000 S4 Reagent Kit (300 cycles) on a NovaSeq 6000 instrument (both Illumina).
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bioRxiv - Cell Biology 2024Quote: ... using the NextSeq® 500/550 High Output Kits v2 (75 cycles; single-end sequencing; Cat# FC-404-2005, Illumina). The FASTQ files were processed using the DolphinNext pipeline [46] on the Massachusetts Green High Performance Computer Cluster (GHPCC) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng of DNA eluate was taken to prepare the NGS library using a TruSeq ChIP sample preparation kit from Illumina. The library was prepared as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... short RNA oligonucleotides were ligated to the ends of the active RNAs to facilitate reverse transcription and PCR needed for sequencing using the TruSeq small RNA library prep kit (Illumina). We adapted the ligation steps to 24h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Clustering of index-coded samples was performed on a cBot Cluster Generation System using the TruSeq SR Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The libraries were prepared by the Duke Center for Genomic and Computational Biology (GCB) using the Tru-Seq stranded total RNA prep kit (Illumina). Sequencing was performed on the NovaSeq 500 by using 75-bp paired-end sequencing.
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bioRxiv - Evolutionary Biology 2024Quote: All qRT-PCR positive samples were sequenced on the same Illumina MiSeq instrument at the Colorado State University Next Generation Sequencing Facility using v2 500 cycle 2 x 250 bp kits (Illumina). The technical replicate libraries for Lion F collected on October 21st were pooled and sequenced as a test run with a separate project ...
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bioRxiv - Genomics 2024Quote: ... The starting point was MNase-digested chromatin isolated from miR combo and control miRNA transfected neonatal cardiac fibroblasts and sequenced on a NovaSeq 6000 Kit (Illumina). The dataset has an accession number of PRJNA837987 in the NIH single-read archive(10) ...
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bioRxiv - Genomics 2024Quote: ... using Illumina’s TruSeq Stranded mRNA HT sample prep kit (Catalog #20020595) utilizing poly-A selection of mRNA following the protocol outlined by Illumina in their user guide ...
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bioRxiv - Genomics 2024Quote: ... An equimolar pool of the libraries was then built up using the TruSeq Stranded mRNA Sample Preparation kit from Illumina. Pair-end sequencing (2 x 150 nt ...
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bioRxiv - Cancer Biology 2024Quote: ... the libraries were sequenced on the NextSeq 500 using a Mid-output 150-cycle Kit in 2×50 configuration to generate 6-8 million read-pairs per sample (Illumina). FastQC were used to examine characteristics of the sequencing libraries ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra DNA Library Prep Kit and sequenced on an HiSeq X Ten platform (Illumina) generating 150 bp paired-end reads.
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bioRxiv - Genomics 2024Quote: ... Sequencing was performed using a molarity of 62.4 nM and 3% PhiX spike in on the Nova Seq 6000 with SP flow cell kit and 200 cycles (Illumina).
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bioRxiv - Genomics 2024Quote: Genomic DNA extracted from nucleated blood cells was utilized for library construction using a Nextera Mate Pair Library Preparation kit from Illumina, following the gel-free protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... using paired-end 75-cycle sequencing run-compatible reagents (150 cycles, NextSeq 500/550 Mid Output v2 sequencing kit; Illumina). Biological triplicates were prepared and sequenced for each growth condition.
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bioRxiv - Genetics 2024Quote: Genomic DNA from each sample was prepared for next generation sequencing using the Nextera DNA Flex Library Prep kit (Illumina). In brief ...