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Citations for Bioline :
1551 - 1600 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA weight marker Hyperladder™ 1-kb (BIoline) and UltraRanger 1kb (Norgen Biotek ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50ng was converted to cDNA using Tetro cDNA synthesis kit (Bioline) with random hexamers ...
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bioRxiv - Genomics 2020Quote: ... 10 μl of each of the 96 Libraries were separately amplified in 20 μl PCR reactions using MyTaq™ (Meridian Bioline, Memphis, Tennessee, USA) and standard Illumina TrueSeq amplification primers (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The normalized library pools were re-amplified in 100μl PCR reactions using MyTaq (Meridian Bioline, Memphis, Tennessee, USA). For each pool ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... PCR using MyTaq polymerase (Bioline BIO-21113) was performed with 3 long extension cycles followed by 17 short extension cycles as described (Vogel ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or the using Bioline Isolate II Genomic DNA extraction kit (Bioline) following the manufacturer’s protocol and suspended in 50 μL elution buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed using SensiMix SYBR low-ROX kit (Bioline) on a QuantStudio5 machine (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... All testing samples were used in qPCR according to SensiFASTTM SYBR® No-ROX Kit manual (Bioline). NvDsx qPCR primers were designed on the common region (exon 2-5 ...
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bioRxiv - Genetics 2020Quote: ... 1 μl forward and reverse primers (10 mM) and 0.5 μl (5 U/μl) BioTaq DNA polymerase (Bioline, London) and amplified using PCR thermal cycler (BiometraTOne ...
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bioRxiv - Genetics 2020Quote: Separation of the amplified product was accomplished in a 4% (w/v) agarose (Bioline, London) gel in 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... and qPCR performed according to the manufacturer’s instruction (Bioline). All primers (Merck ...
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using SensiFAST™ SYBR Hi-ROX Kit (Bioline). rp49 was used as a housekeeping gene for ΔCt calculations ...
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bioRxiv - Cell Biology 2020Quote: ... Cell were propogated in either LB or TB media and protein expression was performed by inducing with 0.5 mM IPTG (Isopropyl ß-D-1-thiogalactopyranoside, Bioline, Cat No. BIO-37036) overnight at 180C ...
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bioRxiv - Cell Biology 2020Quote: ... 25 ng cDNA were used in a total reaction mix of 20 μl containing 10 ul Sensi Fast SYBR Green Hi-Rox (Bioline, London, UK) as well as 400 nM forward and reverse primer (Supplementary Material and Methods ...
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bioRxiv - Genetics 2020Quote: ... Each 20 μl qPCR reaction consisted of 10 μl SensiMix SYBR Low-ROX reagent (Bioline), 1 μl TcZ forward primer (5’-GCTCTTGCCCACAMGGGTGC-3’)50 [10 μM] ...
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bioRxiv - Microbiology 2020Quote: All PCRs were carried out in 25 μl reaction volumes containing 0.05 units of MyTaq™ DNA Polymerase (Bioline Laboratories, London, UK), 1x MyTaq™ Mix (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... 1x MyTaq™ Mix (Bioline) and forward and reverse primers at a concentration of 0.3 μM each ...
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bioRxiv - Genomics 2020Quote: ... PCRs were carried out as 25 µl reactions containing 0.03 units of MyTaq™ (Bioline), 1 × buffer and 0.2 μM of forward and reverse primers and 2 μl of template DNA ...
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bioRxiv - Immunology 2020Quote: ... PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline), and the success of amplification was assessed by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... MyTaq™ Red Mix (Bioline) was used in all other cases ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with Sensimix SYBR Green no-ROX (Bioline) on a Corbett Rotor-Gene 6000 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized from 2.5 μg RNA in a 20 μL reaction using both random hexamers and oligo(dT) primers provided with the Tetro cDNA Synthesis kit (Bioline™). Ten defense genes were quantified using the SYBR Green qRT-PCR kit on a ViiA™ 7 sequence detection system ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV nsp14 mutant expression plasmids were generated by Quikchange site-directed mutagenesis using Accuzyme DNA polymerase (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was induced at an optical density (OD600nm) of 0.8 by adding 50 μM of isopropyl-β-D-a-thiogalactopyranoside (IPTG; Bioline). After 24 h at 13⁰C ...
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bioRxiv - Microbiology 2020Quote: RT-qPCR assays were performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline). Primers and probe for SARS-CoV-2 E gene were taken from the Berlin protocol [2].
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated using 200 μL of parasite culture (Isolate II Genomic DNA kit, Bioline). Candidate actinonin resistance genes were amplified using the primers listed in Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... coli α-select silver efficiency (Bioline) was used to manipulate the pGEM-TEasy library of gene cassettes ...
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bioRxiv - Microbiology 2020Quote: ... MyTaq Red PCR Mix (Bioline, UK) and 200 nM sense/anti-sense primers and amplification following 35 cycles of 95°C ...
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bioRxiv - Microbiology 2020Quote: ... The amplified product was purified using the ISOLATE II PCR and Gel Kit (Bioline, Australia) and cloned into the BamHI and SacI sites of the E ...
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bioRxiv - Microbiology 2020Quote: ... coli were extracted using the ISOLATE II Plasmid Mini kit (Bioline, Australia) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA synthesized with 0.25-1μg of RNA in a 20μL total reaction volume using a random hexamer/oligo dT strand synthesis kit in accordance with the manufacturer’s instructions (10 minutes at 25°C; 15 minutes at 42°C; 15 minutes at 48°C; SensiFast, Bioline). PCR amplification of HBV RNAs were performed using primers as previously described43 using a SYBR green real-time PCR protocol (qPCRBIO SyGreen ...
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bioRxiv - Immunology 2020Quote: ... total RNA was treated with 50 U Tetro reverse transcriptase (Bioline), 40 mM dNTPs (Promega) ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of 1 μg of total RNA was performed using Tetro reverse transcriptase (Bioline). For reverse transcription ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized (Bioline) for subsequent analysis by qPCR.
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bioRxiv - Immunology 2020Quote: ... Target mRNAs were amplified using MyTaq HS DNA Polymerases (Bioline, UK), and amplicons were run on 1% agarose gels ...
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis was performed using the SensiFast cDNA synthesis kit (Bioline, BIO-65054). SensiFast SYBR No-Rox kit (Bioline ...
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bioRxiv - Immunology 2020Quote: ... SensiFast SYBR No-Rox kit (Bioline, BIO-98005) or PrimeTime qPCR Master Mix (IDT ...
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bioRxiv - Immunology 2020Quote: ... SensiFast SYBR No-Rox kit (Bioline; BIO-98005) or PrimeTime qPCR Master Mix (IDT ...
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Disparate regulation of imd drives sex differences in infection pathology in Drosophila melanogasterbioRxiv - Immunology 2020Quote: We used Sensimix with SYBR Green no-ROX (Bioline) or qPCRBIO SyGreen Mix Separate-ROX (PCR Biosystems ...
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis was performed with the SensiFast cDNA synthesis kit (Bioline; BIO-65054). SensiFast SYBR No-Rox kit (Bioline ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed using a BIOTAQ kit (Bioline). Csf1rMeriCreMer mice were genotyped using iCre-F-YL (5’- GTCTCCAACCTGCTGACTGTGC-3’ ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested for cytokine measurement and the cells were lysed (ISOLATE II RNA Lysis Buffer RLY-Bioline) for RNA extraction.
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using Tetro reverse transcription kit (Bioline) and oligo dT 15-mers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reactions (a total volume of 50μl reaction mixture) each consisted of consisting of 10 μl Buffer of 5x MyTaq Reaction Buffer (Bioline, with dye), 0.75μl of each the primers intI1 ...
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bioRxiv - Neuroscience 2020Quote: ... after which cDNA was synthesized using the SensiFAST(tm) cDNA Synthesis Kit (Bioline, Taunton, MA, USA).
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bioRxiv - Genetics 2020Quote: ... 500-1000 μl of TRIsure (Bioline) and 100 μl of zirconia beads were added and the samples were subjected to three cycles of 6,500 rpm with 20 sec breaks on Precellys to crack open the animals ...
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bioRxiv - Genetics 2020Quote: ... 800nM dNTPs (Bioline), 200nM of pfhrp2 primers ...